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Author: Lynne E. Maquat Publisher: Academic Press ISBN: 0080922074 Category : Science Languages : en Pages : 661
Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression Offers step-by-step lab instructions, including necessary equipment and reagents
Author: Lynne E. Maquat Publisher: Academic Press ISBN: 0080922074 Category : Science Languages : en Pages : 661
Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression Offers step-by-step lab instructions, including necessary equipment and reagents
Author: Lynne E. Maquat Publisher: Academic Press ISBN: 0080923321 Category : Science Languages : en Pages : 463
Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway Expert researchers introduce the most advanced technologies and techniques Offers step-by-step lab instructions, including necessary equipment and reagents
Author: Lynne Maquat Publisher: Academic Press ISBN: 9780123743770 Category : Science Languages : en Pages : 564
Book Description
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the difference in processing of mRNA between eukaryotes, bacteria and archea. Benefit: Processing of mRNA differs greatly between eukaryotes, bacteria and archea and this affords researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Benefit: Keeps MIE buyers and online subscribers up-to-date with the latest research Offers step by step lab instructions including necessary equipment and reagents. Benefit: Provides tried and tested techniques which eliminate searching through many different sources. Tested techniques are trustworthy and avoid pitfalls so the same mistakes are not made over and over.
Author: Wenqian Hu Publisher: ISBN: Category : Languages : en Pages : 121
Book Description
The regulation of mRNA translation and degradation is essential for proper gene expression. In eukaryotes, a major mRNA degradation pathway is initiated by deadenylation, followed by decapping, and ultimately 5'-3' exonucleolytic digestion. Removal of the mRNA 5' cap is an irreversible step in mRNA degradation, and is postulated to require dissociation of the mRNA from the ribosomes and packaging into sub-cellular, ribosome-free granules termed P-bodies. Based on this and other observations, a "two-step" model of eukaryotic mRNA degradation had been proposed that mRNA translation and degradation occur in different compartments within the cell. Recent findings suggest, however, that mRNA degradation may occur independent of P-bodies. Consequently, an important but unresolved issue in the field is to determine the context in which mRNA is degraded. In my study, I have demonstrated that the three steps of mRNA decay, deadenylation, decapping, and 5'-3' exonucleolytic digestion, occur co-translationally. Specifically, mRNA deadenylation does not lead to ribosome dissociation. Second, I found that decapped mRNA mainly bound to polyribosomes, suggesting that decapping occurs during translation. In addition, using kinetic analysis, I demonstrated that mRNA decapping is initiated while the mRNA is on polyribosomes. Third, I observed that in wild-type cells, exonucleolytic decay fragments are polyribosome associated when ribosome transit is slowed in cis. Lastly, using an innovative assay I designed, I detected decapping products from endogenous mRNAs mainly on polyribosomes in wild type cells. These results clearly demonstrate that under normal physiological conditions, mRNA degradation occurs while the mRNA is still associated with ribosomes. In addition to the normal mRNA turnover pathway, I observed that mRNA decapping triggered by nonsense-mediated mRNA decay, an important mRNA quality control mechanism, also occurs on polyribosomes. Collectively, these results indicate that polyribosomes are the major sites for destroying both normal and aberrant mRNAs within eukaryotic cells
Author: Torben Heick Jensen Publisher: Springer Science & Business Media ISBN: 1441978410 Category : Medical Languages : en Pages : 161
Book Description
The diversity of RNAs inside living cells is amazing. We have known of the more “classic” RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of this volume to introduce this fascinating protein complex as well as to give a timely and rich account of its many functions. The exosome was discovered more than a decade ago by Phil Mitchell and David Tollervey by its ability to trim the 3’end of yeast, S. cerevisiae, 5. 8S rRNA. In a historic account they laid out the events surrounding this identification and the subsequent birth of the research field. In the chapter by Kurt Januszyk and Christopher Lima the structural organization of eukaryotic exosomes and their evolutionary counterparts in bacteria and archaea are discussed in large part through presentation of structures.
Author: Jon Lorsch Publisher: Academic Press ISBN: 0124200516 Category : Science Languages : en Pages : 472
Book Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on RNA
Author: P. Michael Conn Publisher: Academic Press ISBN: 0123918561 Category : Cell physiology Languages : en Pages : 559
Book Description
This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Publisher's note.
Author: Publisher: Academic Press ISBN: 012391907X Category : Science Languages : en Pages : 593
Book Description
This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Author: Lynne E. Maquat
Author: Lynne E. Maquat
Author: Lynne E. Maquat
Author: Megerditch Kiledjian
Author: Lynne Maquat
Author: Lynne Maquat
Author: Wenqian Hu
Author: Torben Heick Jensen
Author: Jon Lorsch
Author: P. Michael Conn
Author: