RNA Scaffolds PDF Download

Author: Luc Ponchon
Publisher:
ISBN: 9781071614990
Category : Electronic books
Languages : en
Pages : 288

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Book Description
This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field. .

Author: Peixuan Guo
Publisher: CRC Press
ISBN: 1466505664
Category : Medical
Languages : en
Pages : 614

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Book Description
Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

Author: Christina Smolke
Publisher: John Wiley & Sons
ISBN: 3527688099
Category : Science
Languages : en
Pages : 532

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Book Description
A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.

Author:
Publisher: Academic Press
ISBN: 0128045744
Category : Science
Languages : en
Pages : 242

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Book Description
This volume of Progress in Molecular Biology and Translational Science introduces emerging strategies of studying RNA structure and function using nanotechnological tools. The volume aims to provide the readers with a novel view and give them opportunities to think about how to incorporate the new technologies into their own research. This book informs and updates on strategies to take advantages of nanotechnological tools to answer to fundamental questions in RNA science Collection of reviews written by the leading scientists from the field The book covers a range of topics, from single molecule analyses using nanomaterials to gene regulation using RNA nanostructures

Author: Kirill A. Afonin
Publisher: CRC Press
ISBN: 1000263177
Category : Medical
Languages : en
Pages : 1229

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Book Description
This collection of research articles and reviews covers the latest work in the design, delivery, dynamic abilities, and immune stimulation of RNA nanoparticles which have driven the utilization of their immunomodulatory properties. The unknown immune properties of nucleic acid nanoparticles have been a major hurdle in their adaptation until the works herein began assessing their structure-activity relationships. This collection chronologically follows the path of investigating the recognition of design components to implementing them into nucleic acid nanostructures. RNA nanotechnology is an emerging platform for therapeutics with increasing clinical relevance as this approach becomes more widely used and approved for the treatment of various diseases. The latest research aims to take advantage of RNA’s modular nature for the design of nanostructures which can interact with their environments to communicate programmed messages with intracellular pathways. In doing so, nanoparticles can be used to elicit or elude responses by the immune system as desired in conjunction with their therapeutic applications.

Author: Wade William Grabow
Publisher:
ISBN: 9781267767448
Category :
Languages : en
Pages : 275

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Book Description
Over the last several decades ribonucleic acid (RNA) has been found to carry out a variety of previously unrecognized functions within cellular organisms. These discoveries, in conjunction with the first successful structural determination of natural RNA molecules by X-ray and NMR, have generated an interest in RNA outside the traditional bounds of molecular and cellular biology. The ability to control three-dimensional (3-D) space on the nano-scale as well as the inherent and demonstrated abilities of nucleic acids to self-assemble into predictable and controllable materials has caused RNA to gain acceptance and influence in the interdisciplinary fields of synthetic biology, nanotechnology, and nano-medicine. The following work details the guiding principles associated with the design and characterization of both natural and synthetic RNA while highlighting several newly designed RNA-based scaffolds with potential uses in the diverse fields above as well as the emerging field of RNA nanotechnology, which at its core seeks to design, to construct, and to test RNA nanoparticles that can perform specific cellular or biological functions.

Author: Bin Wang
Publisher: CRC Press
ISBN: 9814411647
Category : Medical
Languages : en
Pages : 468

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Book Description
In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.

Author: Kevin D. Rynearson
Publisher:
ISBN: 9781303811418
Category :
Languages : en
Pages : 393

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Book Description
A series of novel benzoxazoles was designed and synthesized with the goal of binding to subdomain IIa of the Hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA and inhibiting viral translation. The benzoxazoles were prepared with fundamental traits which bias these ligands for interaction with RNA. The benzoxazoles were constructed around a recognition element contained in a known subdomain IIa binding ligand that forms discrete hydrogen bonds to the RNA target. This motif within a novel benzoxazole core was anticipated to act as a molecular anchor which orients various substituents, allowing for the exploration of the chemical environment in the subdomain IIa binding cavity. The novel ligands were evaluated by a FRET assay and an in vitro translation assays to determine binding affinity and target inhibition. Structure activity relationships of the substitution patterns were explored. Functional elements which conferred activity were combined around a common benzoxazole scaffold with the goal of creating improved inhibitors.

Author: Navid R. Moheimani
Publisher: Springer
ISBN: 3319166409
Category : Technology & Engineering
Languages : en
Pages : 373

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Book Description
This comprehensive book details the most recent advances in the microalgae biological sciences and engineering technologies for biomass and biofuel production in order to meet the ongoing need for new and affordable sources of food, chemicals and energy for future generations. The chapters explore new microalgae cultivation techniques, including solid (biofilm) systems, and heterotrophic production methods, while also critically investigating topics such as combining wastewater as a source of nutrients, the effect of CO2 on growth, and converting biomass to methane through anaerobic digestion. The book highlights innovative bioproduct optimization and molecular genetic techniques, applications of genomics and metabolomics, and the genetic engineering of microalgae strains targeting biocrude production. The latest developments in microalgae harvesting and dewatering technologies, which combine biomass production with electricity generation, are presented, along with detailed techno-economic modeling. This extensive volume was written by respected experts in their fields and is intended for a wide audience of researchers and engineers.

Author: Vijai Singh
Publisher: Springer Nature
ISBN: 9811500819
Category : Science
Languages : en
Pages : 349

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Book Description
This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.