Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download RNA Editing PDF full book. Access full book title RNA Editing by H. Ulrich Göringer. Download full books in PDF and EPUB format.
Author: H. Ulrich Göringer Publisher: Springer Science & Business Media ISBN: 3540737871 Category : Science Languages : en Pages : 239
Book Description
Goringer’s brilliant new work dedicates a chapter to each of the main types of RNA editing – the very first volume to do so. All of the sections here have been written by experts in the various research areas and a specific focus is put on the correlation between RNA structure and function, as well as on the complex cellular machineries that catalyze the different editing reactions. This leads to a "state of the art" compendium of our current knowledge on RNA editing.
Author: H. Ulrich Göringer Publisher: Springer Science & Business Media ISBN: 3540737871 Category : Science Languages : en Pages : 239
Book Description
Goringer’s brilliant new work dedicates a chapter to each of the main types of RNA editing – the very first volume to do so. All of the sections here have been written by experts in the various research areas and a specific focus is put on the correlation between RNA structure and function, as well as on the complex cellular machineries that catalyze the different editing reactions. This leads to a "state of the art" compendium of our current knowledge on RNA editing.
Author: Albrecht Bindereif Publisher: Springer Science & Business Media ISBN: 3642286879 Category : Science Languages : en Pages : 270
Book Description
Trypanosomes are unicellular protozoa of ancient evolutionary origin that are responsible for several tropical diseases, such as African sleeping sickness. Over the last few decades, research in trypanosome biology has revealed many unique and fascinating features, many of which have helped to establish new paradigms in other biological systems. This applies in particular to studies in gene expression and regulation, which benefit enormously from the trypanosome genome projects and from the new genome-wide approaches recently introduced in trypanosome research. This volume covers the most important aspects of biosynthesis, processing, and functions of RNA in trypanosomes, ranging from transcription to RNA editing, mRNA splicing/translation/turnover, processing of transfer and ribosomal RNA, RNA interference, and current transcriptome-wide analyses. Recent progress in RNA-focused research in trypanosomatids promises to yield novel insights into trypanosome-specific features, as well as to reveal in the process new potential therapeutic strategies for combating these parasitic diseases.
Author: Jonatha M. Gott Publisher: Springer Science & Business Media ISBN: 1592597750 Category : Science Languages : en Pages : 437
Book Description
This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.
Author: Laura Elizabeth Kirby Publisher: ISBN: 9781392031087 Category : Electronic dissertations Languages : en Pages : 301
Book Description
Trypanosoma brucei is a single celled eukaryote that utilizes a complex RNA editing system to render many of its mitochondrial genes translatable. Editing of these genes requires multiple small RNAs called guide RNAs to direct the insertion and deletion of uridines. These gRNAs act sequentially, each generating the anchor binding site for the next gRNA. This sequential dependence should render the process quite fragile, and mutations in the gRNAs should not be tolerated. In the examination of the gRNA transcriptome of T. brucei, many gRNAs were identified that are capable of generating alternative mRNA sequences, and potentially disrupting the editing process. In this work, the effects of alternative editing are characterized. This analysis revealed the role of gRNAs in developmental regulation of gene expression, showing a correlation between the abundance of the initiating gRNAs across two different points in the life cycle of T. brucei and their expression. This study also revealed the existence of mitochondrial dual-coding genes, which provide protection for genetic material that is not under selection at all points of the life cycle of T. brucei. The examination of these dual-coding genes showed that RNA editing patterns can shift between cell lines and under different energetic conditions. Examining the gRNAs involved in these editing pathways revealed that there is a high amount of mismatching base pairs that are tolerated for editing to function, and that gRNA abundance is not a reliable predictor for editing preference. Finally, a reexamination of the gRNA transcriptome revealed that many gRNAs are still unidentified and most likely are generating new alternatively edited sequences.
Author: Alfredo J. Hernandez Publisher: ISBN: Category : Languages : en Pages :
Book Description
RNA editing in trypanosomes is the post-transcriptional insertion or deletion of uridylates at specific sites in mitochondrial mRNAs. This process is catalyzed by a multienzyme, multisubunit complex through a series of enzymatic cycles directed by small, trans-acting RNA molecules. Despite impressive progress in our understanding of the mechanism of RNA editing and the composition of the editing complex, fundamental questions regarding RNP assembly and the regulation of catalysis remain. This dissertation presents studies of RNA-protein interactions between RNA editing complexes and substrate RNAs and the determination of substrate secondary structural determinants that govern them. Our results suggest that substrate association, cleavage and full-round editing by RNA editing complexes in vitro obey hierarchical determinants that increase in complexity as editing progresses and we propose a model for substrate recognition by RNA editing complexes. In addition, this dissertation also presents the characterization of a novel mitochondrial RNA helicase, named REH2 and its macromolecular interactions. Our data suggest that REH2 is intimately involved in interactions with macromolecular complexes that integrate diverse processes mediating mitochondrial gene expression. These results have implications for the mechanism of substrate RNA recognition by RNA editing complexes as well as for the integration of RNA editing to other facets of mitochondrial RNA metabolism.
Author: H. Ulrich Göringer
Author: H. Ulrich Göringer
Author: Hans van der Spek
Author: Nancy Ritsuko Sturm
Author: Johannes Cornelis van der Spek
Author: Albrecht Bindereif
Author: David Speijer
Author: Jonatha M. Gott
Author: Laura N. Rusché
Author: Laura Elizabeth Kirby
Author: Alfredo J. Hernandez