Author: Mirjam Oldenzeel
Publisher:
ISBN: 9789090063546
Category :
Languages : en
Pages : 169

View

Book Description

Author: Philippe Déjardin
Publisher: Springer Science & Business Media
ISBN: 3540326588
Category : Science
Languages : en
Pages : 341

View

Book Description
This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.

Author: Adam Till Garland
Publisher:
ISBN:
Category :
Languages : en
Pages : 230

View

Book Description
The behavior of a protein molecule at the solid-liquid interface is a worthy scientific problem for at least three reasons. The main driving force for studying this problem is a practical one, as many areas of bio-related technologies, such as medical implants, biosensing, and drug delivery, require the understanding of protein-surface interactions. In this dissertation, the nature of the precursive weakly adsorbed state of proteins during binding is reviewed. From this perspective, the adsorption and binding of proteins to a solid block copolymer thin film was achieved with regular spacing. Further efforts produced a monolayer of green fluorescent protein (GFP) covalently bound with regular spacing and orientation to a diblock copolymer thin film. This protein could be folded and refolded by changing solvent characteristics. We also explored the binding of DC-SIGN to mannose and mannotriose bearing lipid membranes. While no binding was observed, the usefulness of the lipid-based glycan microarray was proven using the well-studied CTB-GM1 binding motif.

Author: Martin Malmsten
Publisher: CRC Press
ISBN: 9780824747343
Category : Science
Languages : en
Pages : 930

View

Book Description
This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.

Author: Thomas A. Horbett
Publisher:
ISBN: 9780841233041
Category : Biological interfaces
Languages : en
Pages : 0

View

Book Description
Based on the March 1994 symposium sponsored by the Division of Colloid and Surface Chemistry at the 207th National Meeting held in San Diego, Calif. Contributions cover the theory and molecular mechanisms of protein adsorption (six papers); competitive adsorption of proteins (ten papers); the conformation and orientation of proteins at interfaces (seven papers); the effects of surface chemistry on protein adsorption (six papers); the role of adsorbed proteins in cell interactions with solid surfaces (four papers); and protein behavior at fluid-fluid interfaces (four papers). Some bandw photographs. Annotation copyright by Book News, Inc., Portland, OR

Author: Ananthakrishnan G. Sethuraman
Publisher:
ISBN:
Category :
Languages : en
Pages : 210

View

Book Description

Author: Rehman Fazeem
Publisher:
ISBN:
Category : Amyloid beta-protein
Languages : en
Pages : 185

View

Book Description
"Metropolis Monte Carlo (MC) simulations were performed with positively charged peptides in aqueous solution to study changes in peptide conformations at solid/liquid interface, and its effects on protein aggregation. Intermediate-resolution diblock model peptide, comprising of 10 units of ALA (non-polar) and LYS (polar) amino acid residues, was used for the simulations. In the first approach to modeling, solvent effects were considered explicitly. The explicit model was then used to study two peptide molecules, in helical structure, at solid/liquid interface. In order to increase the number of peptide molecules in the simulation box, with reduced computational cost, an implicit solvent model was developed with nonadditive hydrogen bonding and hydrophobic interaction potentials. The implicit model was used to simulate two peptides of helical structure at charged surfaces (to compare with the explicit model), and ten peptides of random coil structure with and without charged surfaces. The peptides were observed to always move towards the negatively charged surface and orient with residues of complimentary charge settling close to the surface, maximizing the electrostatic interactions. On reaching the surface, the peptides partially lose their secondary structure and clusters around the hydrophobic ends; this restructuring and dehydration of the peptides provides the entropic drive for adsorption and subsequent misfolding events. The 2- peptide-water-surface system in explicit model was also simulated with periodic switching of surface charge polarity, to induce a "shaking effect" in order to observe possible peptide configurational changes"--Abstract, page iii.

Author: Atanas V. Koulov
Publisher: Academic Press
ISBN: 0128125691
Category : Medical
Languages : en
Pages : 236

View

Book Description
Surfactants in Biopharmaceutical Development addresses the progress, challenges and opportunities for surfactant research specific to pharmaceutical development, providing a broad range of important surfactant-related topics as they relate directly to the biopharmaceutical process. Chapters address fundamental topics, like mechanisms of protein stabilization by surfactants, the latest, state-of-the-art technology and methods to illustrate the practical application to biopharmaceutical development, forward-looking chapters on control strategies and novel surfactants, with a special focus on current regulatory aspects of paramount importance for biopharmaceutical companies and regulators. It has been widely recognized that surfactants provide protection to therapeutic proteins against interfacial stresses. Despite the fact that the very mechanism of protein stabilization by surfactants has not been completely understood, surfactants are universally regarded as critical functional excipients by the industry and by regulators. Describes the current state of research on surfactants in the context of biopharmaceutical development, drawing upon contributions from international experts across industry, academia, and regulators Addresses the opportunities and challenges associated with surfactants in biologic drug development Provides a defining resource for practitioners in the biopharmaceutical industry, regulators and academics by summarizing the latest knowledge of surfactants in biopharmaceutical development in one comprehensive volume

Author: Masahiko Utsuro
Publisher: John Wiley & Sons
ISBN: 9783527628797
Category : Technology & Engineering
Languages : en
Pages : 610

View

Book Description
Written by authors with an international reputation, acknowledged expertise and teaching experience, this is the most up-to-date resource on the field. The text is clearly structured throughout so as to be readily accessible, and begins by looking at scattering of a scalar particle by one-dimensional systems. The second section deals with the scattering of neutrons with spin in one-dimensional potentials, while the third treats dynamical diffraction in three-dimensional periodic media. The final two sections conclude with incoherent and small angle scattering, and some problems of quantum mechanics. With its treatment of the theories, experiments and applications involved in neutron optics, this relevant reading for nuclear physicists and materials scientists alike.

Author: Waldemar Alfredo Monteiro
Publisher: BoD – Books on Demand
ISBN: 9535122762
Category : Science
Languages : en
Pages : 160

View

Book Description
This book brings suitable data concerning theory and experiments of neutron interactions with different materials. Since the neutron discovery by Chadwick in 1932, researchers of the entire world begin to make studies about it. It is well known that neutron have no charge, and their electric dipole moment is either zero or too small to measure, but theories and experiments show that neutron has spin (presence of magnetic moment), and polarization neutron scattering is plausible. The reader can obtain remarks about inelastic scattering cross sections for neutron; polarized neutron reflectivity; scattering methods; neutron reflectometry tool to probe the chemical structures; neutron scattering for amino acid crystals; and small-angle neutron scattering nanoemulsion heat transfer fluids in this book.