Author: Chanika Manmontri
Publisher:
ISBN:
Category :
Languages : en
Pages : 53
Book Description
Mutans streptococci (MS), e.g., Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss), are associated with dental caries. Quantification of MS has been shown to predict caries risk. Traditional culture methods are problematic, especially because consistently maintaining the viability of bacteria is difficult. A new technology using PCR has the potential to streamline and improve MS quantification compared to traditional culture methods. Purpose: To use real-time quantitative PCR (qPCR) and standard plate counting (SPC) for detection and quantification of levels of Sm, Ss and total streptococci (TS) in clinical samples from high-caries-risk children (no regular access to dental care) and to evaluate the correlation of these results to caries prevalence. Methods: Saliva and plaque samples were collected from school-aged children for SYBR Green based qPCR using primers specific for Sm, Ss and TS from DNA extracted. UA159 genomic DNA was used as standard from which "copy number" (CN/ml) of MS and TS was computed. For comparison, samples were also processed and quantified for "colony forming unit" (CFU/ml) of MS and TS using SPC. Calibrated examiners performed oral examinations (DMFT/DMFS/dmft/dmfs). Pearson's analysis and T-test assessed correlations between level of Sm and ratio of Sm to TS to caries using SAS V9.2. Results: Fifty-eight subjects (mean age=9.8 years, DMFS/dmfs=7.0) had samples collected. By qPCR Sm was detected in 98.1% of samples while SPC was 72.9%. Ss was only found in 4 qPCR samples (none by SPC). Mean qPCR CN/ml of samples were: Sm (2.54x106), Ss (5.27x102) and TS (2.34x109). Mean SPC CFU/ml of samples were: Sm (9.4x105), Ss (0) and TS (1.0x107). Significant correlations were found between level of Sm and ratio of Sm/TS from oral samples to prevalence of dental caries. Conclusion: The levels of Sm and ratio of Sm/TS by qPCR and SPC were correlated to dental caries. Correlations between mean levels of Sm and ratio of Sm/TS to caries experience from qPCR are comparable to SPC. Given the similar correlations found, the more streamlined method of qPCR is a suitable outcome measure in epidemiological studies.
Real-time Quantitative PCR for Evaluation of Mutans Streptococci and Dental Caries
Author: Chanika Manmontri
Publisher:
ISBN:
Category :
Languages : en
Pages : 53
Book Description
Mutans streptococci (MS), e.g., Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss), are associated with dental caries. Quantification of MS has been shown to predict caries risk. Traditional culture methods are problematic, especially because consistently maintaining the viability of bacteria is difficult. A new technology using PCR has the potential to streamline and improve MS quantification compared to traditional culture methods. Purpose: To use real-time quantitative PCR (qPCR) and standard plate counting (SPC) for detection and quantification of levels of Sm, Ss and total streptococci (TS) in clinical samples from high-caries-risk children (no regular access to dental care) and to evaluate the correlation of these results to caries prevalence. Methods: Saliva and plaque samples were collected from school-aged children for SYBR Green based qPCR using primers specific for Sm, Ss and TS from DNA extracted. UA159 genomic DNA was used as standard from which "copy number" (CN/ml) of MS and TS was computed. For comparison, samples were also processed and quantified for "colony forming unit" (CFU/ml) of MS and TS using SPC. Calibrated examiners performed oral examinations (DMFT/DMFS/dmft/dmfs). Pearson's analysis and T-test assessed correlations between level of Sm and ratio of Sm to TS to caries using SAS V9.2. Results: Fifty-eight subjects (mean age=9.8 years, DMFS/dmfs=7.0) had samples collected. By qPCR Sm was detected in 98.1% of samples while SPC was 72.9%. Ss was only found in 4 qPCR samples (none by SPC). Mean qPCR CN/ml of samples were: Sm (2.54x106), Ss (5.27x102) and TS (2.34x109). Mean SPC CFU/ml of samples were: Sm (9.4x105), Ss (0) and TS (1.0x107). Significant correlations were found between level of Sm and ratio of Sm/TS from oral samples to prevalence of dental caries. Conclusion: The levels of Sm and ratio of Sm/TS by qPCR and SPC were correlated to dental caries. Correlations between mean levels of Sm and ratio of Sm/TS to caries experience from qPCR are comparable to SPC. Given the similar correlations found, the more streamlined method of qPCR is a suitable outcome measure in epidemiological studies.
Publisher:
ISBN:
Category :
Languages : en
Pages : 53
Book Description
Mutans streptococci (MS), e.g., Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss), are associated with dental caries. Quantification of MS has been shown to predict caries risk. Traditional culture methods are problematic, especially because consistently maintaining the viability of bacteria is difficult. A new technology using PCR has the potential to streamline and improve MS quantification compared to traditional culture methods. Purpose: To use real-time quantitative PCR (qPCR) and standard plate counting (SPC) for detection and quantification of levels of Sm, Ss and total streptococci (TS) in clinical samples from high-caries-risk children (no regular access to dental care) and to evaluate the correlation of these results to caries prevalence. Methods: Saliva and plaque samples were collected from school-aged children for SYBR Green based qPCR using primers specific for Sm, Ss and TS from DNA extracted. UA159 genomic DNA was used as standard from which "copy number" (CN/ml) of MS and TS was computed. For comparison, samples were also processed and quantified for "colony forming unit" (CFU/ml) of MS and TS using SPC. Calibrated examiners performed oral examinations (DMFT/DMFS/dmft/dmfs). Pearson's analysis and T-test assessed correlations between level of Sm and ratio of Sm to TS to caries using SAS V9.2. Results: Fifty-eight subjects (mean age=9.8 years, DMFS/dmfs=7.0) had samples collected. By qPCR Sm was detected in 98.1% of samples while SPC was 72.9%. Ss was only found in 4 qPCR samples (none by SPC). Mean qPCR CN/ml of samples were: Sm (2.54x106), Ss (5.27x102) and TS (2.34x109). Mean SPC CFU/ml of samples were: Sm (9.4x105), Ss (0) and TS (1.0x107). Significant correlations were found between level of Sm and ratio of Sm/TS from oral samples to prevalence of dental caries. Conclusion: The levels of Sm and ratio of Sm/TS by qPCR and SPC were correlated to dental caries. Correlations between mean levels of Sm and ratio of Sm/TS to caries experience from qPCR are comparable to SPC. Given the similar correlations found, the more streamlined method of qPCR is a suitable outcome measure in epidemiological studies.
Streptococcus Mutans and Dental Caries
On the Epidemiology of Mutans Streptococci
Author: Peter Carlsson
Publisher:
ISBN:
Category : Dental caries
Languages : en
Pages : 112
Book Description
Publisher:
ISBN:
Category : Dental caries
Languages : en
Pages : 112
Book Description
Streptococcus Mutans Establishment and Changes in Salivary IgA in Young Children with Reference to Dental Caries
Author: Satu Alaluusua
Publisher:
ISBN:
Category : Dental caries
Languages : en
Pages : 110
Book Description
Publisher:
ISBN:
Category : Dental caries
Languages : en
Pages : 110
Book Description
On Mutans Streptococci in Margins of Restorations
Author: Catarina Wallman
Publisher:
ISBN:
Category : Chlorhexidine
Languages : en
Pages : 104
Book Description
Publisher:
ISBN:
Category : Chlorhexidine
Languages : en
Pages : 104
Book Description
PCR Detection of Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque Samples from Low, Moderate, and High Caries Risk Children
Mutans Streptococci - in Families And on Tooth Sites
Author: Ing-Mari Redmo Emanuelsson
Publisher: Swedish Dental Assn
ISBN: 9789162848262
Category : Medical
Languages : en
Pages : 66
Book Description
Publisher: Swedish Dental Assn
ISBN: 9789162848262
Category : Medical
Languages : en
Pages : 66
Book Description
Molecular Detection of Human Bacterial Pathogens
Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1439812381
Category : Medical
Languages : en
Pages : 1282
Book Description
As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current diagnostic approaches for major human bacterial pathogens. Each chapter consists of a brief review on the classification, epidemiology, clinical features, and diagnosis of an important pathogenic bacterial genus, an outline of clinical sample collection and preparation procedures, a selection of representative stepwise molecular protocols, and a discussion on further research requirements relating to improved diagnosis. This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology.
Publisher: CRC Press
ISBN: 1439812381
Category : Medical
Languages : en
Pages : 1282
Book Description
As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current diagnostic approaches for major human bacterial pathogens. Each chapter consists of a brief review on the classification, epidemiology, clinical features, and diagnosis of an important pathogenic bacterial genus, an outline of clinical sample collection and preparation procedures, a selection of representative stepwise molecular protocols, and a discussion on further research requirements relating to improved diagnosis. This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology.
Oral Biofilms
Author: S. Eick
Publisher: Karger Medical and Scientific Publishers
ISBN: 3318068527
Category : Medical
Languages : en
Pages : 247
Book Description
Biofilms are highly organized polymicrobial communities that are embedded in an extracellular matrix and formed on natural and artificial surfaces. In the oral cavity, biofilms are formed not only on natural teeth, but also on restorative materials, prosthetic constructions, and dental implants. Oral diseases like caries, gingivitis, periodontitis, and also pulp inflammation are associated with biofilms. This publication is an up-to-date overview on oral biofilms from different clinically relevant perspectives. Experts comprising basic researchers and clinicians report on recent research relating to biofilms - from general summaries to recommendations for daily clinical work. This book covers all aspects of oral biofilms, including models used in the laboratory, biofilms in dental water unit lines, periodontal and peri-implant biofilms, caries-related biofilms, halitosis, endodontic biofilms, and Candida infections, as well as biofilms on dental materials and on orthodontic appliances. Several chapters deal with anti-biofilm therapy, from the efficacy of mechanical methods and the use of antimicrobials, to alternative concepts. This publication is particularly recommended to dental medicine students, practitioners, other oral healthcare professionals, and scientists with an interest in translational research on biofilms.
Publisher: Karger Medical and Scientific Publishers
ISBN: 3318068527
Category : Medical
Languages : en
Pages : 247
Book Description
Biofilms are highly organized polymicrobial communities that are embedded in an extracellular matrix and formed on natural and artificial surfaces. In the oral cavity, biofilms are formed not only on natural teeth, but also on restorative materials, prosthetic constructions, and dental implants. Oral diseases like caries, gingivitis, periodontitis, and also pulp inflammation are associated with biofilms. This publication is an up-to-date overview on oral biofilms from different clinically relevant perspectives. Experts comprising basic researchers and clinicians report on recent research relating to biofilms - from general summaries to recommendations for daily clinical work. This book covers all aspects of oral biofilms, including models used in the laboratory, biofilms in dental water unit lines, periodontal and peri-implant biofilms, caries-related biofilms, halitosis, endodontic biofilms, and Candida infections, as well as biofilms on dental materials and on orthodontic appliances. Several chapters deal with anti-biofilm therapy, from the efficacy of mechanical methods and the use of antimicrobials, to alternative concepts. This publication is particularly recommended to dental medicine students, practitioners, other oral healthcare professionals, and scientists with an interest in translational research on biofilms.
Clinical Cases in Orthodontics
Author: Martyn T. Cobourne
Publisher: John Wiley & Sons
ISBN: 140519779X
Category : Medical
Languages : en
Pages : 457
Book Description
Wiley-Blackwell's Clinical Cases series is designed to recognize the centrality of clinical cases to the profession by providing actual cases with an academic backbone. Clinical Cases in Orthodontics applies both theory and practice to real-life orthodontic cases in a clinically relevant format. This unique approach supports the new trend in case-based and problem-based learning, thoroughly covering topics ranging from Class I malocclusions to orthognathic surgery. Highly illustrated in full color, Clinical Cases in Orthodontics' format fosters independent learning and prepares the reader for case-based examinations.
Publisher: John Wiley & Sons
ISBN: 140519779X
Category : Medical
Languages : en
Pages : 457
Book Description
Wiley-Blackwell's Clinical Cases series is designed to recognize the centrality of clinical cases to the profession by providing actual cases with an academic backbone. Clinical Cases in Orthodontics applies both theory and practice to real-life orthodontic cases in a clinically relevant format. This unique approach supports the new trend in case-based and problem-based learning, thoroughly covering topics ranging from Class I malocclusions to orthognathic surgery. Highly illustrated in full color, Clinical Cases in Orthodontics' format fosters independent learning and prepares the reader for case-based examinations.