Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture PDF Download

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Publisher:
ISBN:
Category :
Languages : en
Pages : 288

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Author: Edgardo Abelardo Dohns
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Rationally designed polypeptide-based materials have advantages over other biomaterials for their ability to be engineered from the bottom-up, and to be tailored to the needs of specific potential applications. Based on previous works in the Woolfson laboratory on self- assembling peptide fibres (SAF), this research project focused on developing coiled-coil- based fibrillar hydrogel networks called hydrogelating SAFs (hSAFs), and applying them to more-complex biological systems, notably in cell culture. The hSAFs were tested as a three- dimensional cell-culture scaffold with the aim of mimicking the microenvironment provided by native extracellular matrix (ECM). hSAFs were shown to support growth and differentiation of neuronal cells. Furthermore, a design strategy was explored on how to couple short biomimetic peptides to these proteinogenic scaffolds to enhance cell-matrix interactions. This was achieved by integrating the well-studied cell-adhesion motif RGDS (Arg-Gly-Asp- Ser) to hSAFs using bioorthogonal copper-based click chemistry. Addition of such biomimetic peptides did not alter folding, fibre formation, and higher-order assembly (gelation) of the hSAFs. The resulting functionalised gels promoted early differentiation and longer neurite-like outgrowth in PC12 cells, and of primary rat hippocampal cells. The attached biomimetic peptide was functional and elicited the desired cellular response. Another short biomimetic peptides, i.e. the laminin-derived IKVAV (lIe-Lys-Val-Ala-Val), which supports neurite growth, was also successfully incorporated into hSAFs and gels. Thus, we have demonstrated a chemically defined, bottom-up approach to biological scaffold synthesis and assembly, and further the use of straightforward techniques to incorporate specific biomolecular signals under physiological conditions. Although conditions will need to be optimised for different cell types, hSAF gel formation and functionalisation via click chemistry promise a robust platform in cell-culture studies and potentially for tissue engineering.

Author: Toshiki Asao
Publisher: BoD – Books on Demand
ISBN: 9535132415
Category : Science
Languages : en
Pages : 296

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Amino Acid - New Insights and Roles in Plant and Animal provides useful information on new aspects of amino acid structure, synthesis reactions, dietary application in animals, and metabolism in plants. Section 1 includes chapters that describe the therapeutic uses, antiallergic effects, new aspects in the D-amino acid structure, historical background of desmosines, and stereoselective synthesis of ?-aminophosphonic acids. Section 2 presents the role of amino acids in plants, which includes new insights and aspects of D-amino acids, metabolism and transport in soybean, changes during energy storage compound accumulation of microalgae, and determination of amino acids from natural compounds. Section 3 describes the chapters on methodologies and requirement of dietary amino acids for Japanese quails, laying hens, and finishing pigs. The final chapter identifies potential importance of glutathione S-transferase activity for generating resistance to triclabendazole in Fasciola hepatica.

Author: Richard G. Weiss
Publisher: Springer Science & Business Media
ISBN: 1402036892
Category : Science
Languages : en
Pages : 969

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"Molecular Gels: Materials with Self-Assembled Fibrillar Networks" is a comprehensive treatise on gelators, especially low molecular-mass gelators and the properties of their gels. The structures and modes of formation of the self-assembled fibrillar networks (SAFINs) that immobilize the liquid components of the gels are discussed experimentally and theoretically. The spectroscopic, rheological, and structural features of the different classes of low molecular-mass gelators are also presented. Many examples of the application of the principal analytical techniques for investigation of molecular gels (including SANS, SAXS, WAXS, UV-vis absorption, fluorescence and CD spectroscopies, scanning electron, transmission electron and optical microscopies, and molecular modeling) are presented didactically and in-depth, as are several of the theories of the stages of aggregation of individual low molecular-mass gelator molecules leading to SAFINs. Several actual and potential applications of molecular gels in disparate fields (from silicate replication of nanostructures to art conservation) are described. Special emphasis is placed on perspectives for future developments. This book is an invaluable resource for researchers and practitioners either already researching self-assembly and soft matter or new to the area. Those who will find the book useful include chemists, engineers, spectroscopists, physicists, biologists, theoreticians, and materials scientists.

Author: W. Chan
Publisher: OUP Oxford
ISBN: 0199637245
Category : Science
Languages : en
Pages : 371

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Since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The focus of this new volume is much broader, and covers the essential procedures.

Author: Paolo Ugo
Publisher: MDPI
ISBN: 3038424064
Category : Science
Languages : en
Pages : 187

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This book is a printed edition of the Special Issue "Electrochemical Immunosensors and Aptasensors" that was published in Chemosensors

Author: Thimmaiah Govindaraju
Publisher:
ISBN: 9789811641909
Category :
Languages : en
Pages : 0

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This book is the ultimate assembly of recent research activities on molecular architectonics and nanoarchitectonics by authors who are worldwide experts. The book proposes new ways of creating functional materials at the nano level using the concepts of molecular architectonics and nanoarchitectonics, which are expected to be the next-generation approaches beyond conventional nanotechnology. All the contents are categorized by types of materials, organic materials, biomaterials, and nanomaterials. For that reason, non-specialists including graduate and undergraduate students can start reading the book from any points they would like. Cutting-edge trends in nanotechnology and material sciences are easily visible in the contents of the book, which is highly useful for both students and experimental materials scientists. .

Author: Gyula Palyi
Publisher: Elsevier
ISBN: 0080474039
Category : Science
Languages : en
Pages : 445

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Following on from Advances in BioChirality, Progress in Biological Chirality provides a unique summary and review of the most recent developments in the field of biochirality. Living organisms use only one enantiomer of chiral molecules in the majority of biologically important processes. The exact origin and mechanisms for this surprising selectivity are not yet known. This book discusses current research aimed at identifying the scientific reasons that may contribute to this phenomenon. Progress in Biological Chirality takes an interdisciplinary approach to this exciting field, covering a wide range of topics, such as, theory, palaeontology and food technology, to name but a few. This book presents findings via a broad spectrum of scientific approaches making it an excellent overview of Biological Chirality, suitable for postgraduate students, practitioners and researchers in the field of chemistry, biochemistry, biology, palaeontology, and food science with an interest in Chirality. This book contains 32 chapters written by Authors, who are leading authorities in the field Presents the most recent research taking place in this highly challenging field Contains both reference material for the specialist and provides an overview for those who are interested in the fundamental problems of biology and chemistry

Author: Mihrimah Ozkan
Publisher: Springer Science & Business Media
ISBN: 0387258434
Category : Technology & Engineering
Languages : en
Pages : 554

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Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices with applications to Genomics and Proteomics. Topics include gene expression profiling utilizing microarray technology; imaging and sensing for gene detection and use in DNA analysis; and coverage of advanced microfluidic devices and the Humane Genome Project.

Author: Wolfgang Aehle
Publisher: John Wiley & Sons
ISBN: 9783527316892
Category : Science
Languages : en
Pages : 520

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Weltweit führende Experten geben einen aktuellen Überblick über den gesamten Bereich der Herstellung und Verwendung von Enzymen in der Industrie: - bei der Herstellung von Massengütern wie Glukose oder Fruktose - in der Textil-, Papier-, Nahrungsmittel und Tierfutterindustrie - in der klinischen Diagnose und Therapie - in der Gentechnologie In mehreren ausführlichen Tabellen sind zahlreiche Daten von Enzymen zusammengestellt. Ein umfassendes Literaturverzeichnis ermöglicht einen schellen Zugriff auf Primärliteratur. Gesetzliche Bestimmungen für den sicheren und legalen Gebrauch von Enzymen runden das Buch an. Aus Rezensionen voriger Auflagen: 'Die Qualität...ist so hervorragend, dass es uneingeschränkt als ideale Lektüre jedem Studenten, der eine Einführung in das Gebiet sucht, empfohlen werden kann. ...'Enzymes in Industry' sollte seinen Platz in jeder Bibliothek haben, sei es in der Industrie oder Hochschule, wo es mit Sicherheit oft seinen Nutzen finden wird.' (The Genetic Engineer and Biotechnologist) 'Das Buch ist auf dem neuesten Stand und trotz der Beteiligung von fast 50 Autoren leicht zu lesen. ... Naturwissenschaftler, die sich mit Enzymen beschäftigen, sollten dieses Buch in ihrer Bibliothek haben. Das Buch wird aber auch für den Marketing-Spezialisten, der sich einen Überblick über gegenwärtige und künftige Anwendungen von Enzymen innerhalb und außerhalb der Lebensmittelindustrie machen will, von großem Nutzen sein.' (Angewandte Chemie)