Author: Adrienne Marie Gorny
Publisher:
ISBN:
Category :
Languages : en
Pages : 207
Book Description
The Northern root-knot nematode, Meloidogyne hapla, is an important soilborne pathogen of potato in New York State. Initial pathogen populations (Pi) are often inversely correlated with crop loss and damage, with the economic threshold representing the Pi likely to result in significant crop loss, thus justifying use of control tactics. However, the economic threshold for M. hapla in potato in New York State is not well understood. Furthermore, standard methods of enumerating populations of nematodes from soil are time consuming and error prone. This research sought to quantify the risk of crop loss associated with M. hapla Pi, and develop a DNA-based soil test for prediction of risk. A method for isolating nematode DNA from 100 g of soil using super paramagnetic iron oxide nanoparticles was developed and tested using artificially inoculated soil and field collected samples. A species-specific, real-time quantitative PCR (qPCR) assay targeting the root-knot nematode effector gene 16D10 was developed for quantifying M. hapla. Spatial and spatiotemporal analysis of M. hapla and root-lesion nematodes, Pratylenchus spp., was conducted by intensively sampling three commercial potato fields in New York State. This informed enhanced sampling strategies and evaluated the potential for site-specific nematicide application. The influence of M. hapla Pi on crop loss was assessed by quantifying M. hapla Pi in commercial potato fields through manual extraction and a nested qPCR assay, and investigating associations with yield components. M. hapla Pi did not have a significant effect on total yield, number of tubers, tuber diameter, or tuber galling severity, suggesting the economic threshold for M. hapla in potato is higher than previously regarded (approximately 100 M. hapla second-stage juveniles (J2s) per 100 g soil), and/or tolerant cultivars were used. The response of the commercial potato cvs. Eva and Lamoka to differing Pi of M. hapla and P. penetrans was assessed through replicated field trials. Yield was not impacted even at the highest Pi tested (500 M. hapla J2s or 1,000 P. penetrans nematodes). These results will be used to guide future research to refine economic thresholds, explore advanced nematode management options, and assess potato cultivar susceptibility to plant-parasitic nematodes.
Quantifying Risk for the Northern Root-knot Nematode, Meloidogyne Hapla, in Potato in New York State
Author: Adrienne Marie Gorny
Publisher:
ISBN:
Category :
Languages : en
Pages : 207
Book Description
The Northern root-knot nematode, Meloidogyne hapla, is an important soilborne pathogen of potato in New York State. Initial pathogen populations (Pi) are often inversely correlated with crop loss and damage, with the economic threshold representing the Pi likely to result in significant crop loss, thus justifying use of control tactics. However, the economic threshold for M. hapla in potato in New York State is not well understood. Furthermore, standard methods of enumerating populations of nematodes from soil are time consuming and error prone. This research sought to quantify the risk of crop loss associated with M. hapla Pi, and develop a DNA-based soil test for prediction of risk. A method for isolating nematode DNA from 100 g of soil using super paramagnetic iron oxide nanoparticles was developed and tested using artificially inoculated soil and field collected samples. A species-specific, real-time quantitative PCR (qPCR) assay targeting the root-knot nematode effector gene 16D10 was developed for quantifying M. hapla. Spatial and spatiotemporal analysis of M. hapla and root-lesion nematodes, Pratylenchus spp., was conducted by intensively sampling three commercial potato fields in New York State. This informed enhanced sampling strategies and evaluated the potential for site-specific nematicide application. The influence of M. hapla Pi on crop loss was assessed by quantifying M. hapla Pi in commercial potato fields through manual extraction and a nested qPCR assay, and investigating associations with yield components. M. hapla Pi did not have a significant effect on total yield, number of tubers, tuber diameter, or tuber galling severity, suggesting the economic threshold for M. hapla in potato is higher than previously regarded (approximately 100 M. hapla second-stage juveniles (J2s) per 100 g soil), and/or tolerant cultivars were used. The response of the commercial potato cvs. Eva and Lamoka to differing Pi of M. hapla and P. penetrans was assessed through replicated field trials. Yield was not impacted even at the highest Pi tested (500 M. hapla J2s or 1,000 P. penetrans nematodes). These results will be used to guide future research to refine economic thresholds, explore advanced nematode management options, and assess potato cultivar susceptibility to plant-parasitic nematodes.
Publisher:
ISBN:
Category :
Languages : en
Pages : 207
Book Description
The Northern root-knot nematode, Meloidogyne hapla, is an important soilborne pathogen of potato in New York State. Initial pathogen populations (Pi) are often inversely correlated with crop loss and damage, with the economic threshold representing the Pi likely to result in significant crop loss, thus justifying use of control tactics. However, the economic threshold for M. hapla in potato in New York State is not well understood. Furthermore, standard methods of enumerating populations of nematodes from soil are time consuming and error prone. This research sought to quantify the risk of crop loss associated with M. hapla Pi, and develop a DNA-based soil test for prediction of risk. A method for isolating nematode DNA from 100 g of soil using super paramagnetic iron oxide nanoparticles was developed and tested using artificially inoculated soil and field collected samples. A species-specific, real-time quantitative PCR (qPCR) assay targeting the root-knot nematode effector gene 16D10 was developed for quantifying M. hapla. Spatial and spatiotemporal analysis of M. hapla and root-lesion nematodes, Pratylenchus spp., was conducted by intensively sampling three commercial potato fields in New York State. This informed enhanced sampling strategies and evaluated the potential for site-specific nematicide application. The influence of M. hapla Pi on crop loss was assessed by quantifying M. hapla Pi in commercial potato fields through manual extraction and a nested qPCR assay, and investigating associations with yield components. M. hapla Pi did not have a significant effect on total yield, number of tubers, tuber diameter, or tuber galling severity, suggesting the economic threshold for M. hapla in potato is higher than previously regarded (approximately 100 M. hapla second-stage juveniles (J2s) per 100 g soil), and/or tolerant cultivars were used. The response of the commercial potato cvs. Eva and Lamoka to differing Pi of M. hapla and P. penetrans was assessed through replicated field trials. Yield was not impacted even at the highest Pi tested (500 M. hapla J2s or 1,000 P. penetrans nematodes). These results will be used to guide future research to refine economic thresholds, explore advanced nematode management options, and assess potato cultivar susceptibility to plant-parasitic nematodes.
The Root-knot Nematode (Heterodera Marioni) in Relation to the Potato Industry on Long Island
Author: H. S. Cunningham
Publisher:
ISBN:
Category : Potatoes
Languages : en
Pages : 32
Book Description
Introduction; Life history of the root-knot nemtode; Temperature relations Symptoms on potatoes; Root infestation; Tuber infestation; varietal susceptibility; Effect on growth; Effect on marketing; Effect of storage; depth in soils; Possibility of spread; Field hosts; Control measures; Conclusions.
Publisher:
ISBN:
Category : Potatoes
Languages : en
Pages : 32
Book Description
Introduction; Life history of the root-knot nemtode; Temperature relations Symptoms on potatoes; Root infestation; Tuber infestation; varietal susceptibility; Effect on growth; Effect on marketing; Effect of storage; depth in soils; Possibility of spread; Field hosts; Control measures; Conclusions.
Major Potato Diseases and Nematodes
Author: International Potato Center
Publisher: International Potato Center
ISBN:
Category :
Languages : en
Pages : 72
Book Description
Publisher: International Potato Center
ISBN:
Category :
Languages : en
Pages : 72
Book Description
Variability of Root-knot Nematodes and the Nature of Potato Resistance to These Nematodes
Author: Manuel Antonio Canto-Saenz
Publisher:
ISBN:
Category : Potatoes
Languages : en
Pages : 242
Book Description
Publisher:
ISBN:
Category : Potatoes
Languages : en
Pages : 242
Book Description
Resistance to Root-knot Nematodes, Meloidogyne Spp., in Potato
Author: Geert J. W. Janssen
Publisher:
ISBN: 9789054856764
Category :
Languages : en
Pages : 111
Book Description
Publisher:
ISBN: 9789054856764
Category :
Languages : en
Pages : 111
Book Description
Studies on the Association Between Resistance to the Root-knot Nematode (Meloidogyne Incognita) and a Necrotic Reaction to Infection by Strain M[superscript S]N[superscript R] of Potato Virus Y in Nicotiana Tabacum
Multifaceted Biocontrol Methods Against the Columbia Root Knot Nematode, Meloidogyne Chitwoodi, and the Colorado Potato Beetle, Leptinotarsa Decemlineata, Pests of Potatoes in Washington State
Author: Donna Renee Henderson
Publisher:
ISBN:
Category : Colorado potato beetle
Languages : en
Pages : 84
Book Description
Publisher:
ISBN:
Category : Colorado potato beetle
Languages : en
Pages : 84
Book Description
Potato Situation and the Status of Potato Research in New York
Author: New York State College of Agriculture. Extension Service
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Bibliography of Agriculture
The Guava Root-knot Nematode (Meloidogyne Enterlobii), a Potential Threat to Arkansas Sweet Potatoes and Other Crops
Author: Terrence L. Kirkpatrick
Publisher:
ISBN:
Category : Nematode diseases of plants
Languages : en
Pages : 2
Book Description
Publisher:
ISBN:
Category : Nematode diseases of plants
Languages : en
Pages : 2
Book Description