QTL Mapping of Phytophthora Capsici Resistance and Horticultural Traits in a Pepper (Capsicum Annuum) Recombinant Inbred Line Population PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download QTL Mapping of Phytophthora Capsici Resistance and Horticultural Traits in a Pepper (Capsicum Annuum) Recombinant Inbred Line Population PDF full book. Access full book title QTL Mapping of Phytophthora Capsici Resistance and Horticultural Traits in a Pepper (Capsicum Annuum) Recombinant Inbred Line Population by Michelle Renee Curtis. Download full books in PDF and EPUB format.
Author: Michelle Renee Curtis Publisher: ISBN: 9781321608335 Category : Languages : en Pages :
Book Description
Root rot disease, caused by Phytophthora capsici, is a major disease of pepper (Capsicum annuum), an important vegetable crop. P. capsici is widespread in the US and increasingly in other pepper production areas of the world. Breeding of resistant pepper varieties with acceptable horticultural attributes has been difficult due to the quantitative nature of resistance, negative traits in resistant donor lines, and the genetic diversity of the pathogen. The main goals of this study were to locate P. capsici resistance QTL that are effective against diverse P. capsici isolates and determine linkage relationships among QTL for horticultural traits and resistance QTL. An F6 recombinant inbred line (RIL) population (n = 257), derived from an intraspecific Capsicum annuum F1 cross between P. capsici resistant Criollo de Morellos-334 and an elite dihaploid ancho breeding line, was evaluated in multiple disease screens using five different P. capsici isolates and in the field for horticultural traits (fruit length, fruit width, fruit shape, 10-fruit weight, and maturity). Twenty-five significant QTL (P
Author: Michelle Renee Curtis Publisher: ISBN: 9781321608335 Category : Languages : en Pages :
Book Description
Root rot disease, caused by Phytophthora capsici, is a major disease of pepper (Capsicum annuum), an important vegetable crop. P. capsici is widespread in the US and increasingly in other pepper production areas of the world. Breeding of resistant pepper varieties with acceptable horticultural attributes has been difficult due to the quantitative nature of resistance, negative traits in resistant donor lines, and the genetic diversity of the pathogen. The main goals of this study were to locate P. capsici resistance QTL that are effective against diverse P. capsici isolates and determine linkage relationships among QTL for horticultural traits and resistance QTL. An F6 recombinant inbred line (RIL) population (n = 257), derived from an intraspecific Capsicum annuum F1 cross between P. capsici resistant Criollo de Morellos-334 and an elite dihaploid ancho breeding line, was evaluated in multiple disease screens using five different P. capsici isolates and in the field for horticultural traits (fruit length, fruit width, fruit shape, 10-fruit weight, and maturity). Twenty-five significant QTL (P
Author: Jareerat Chunthawodtiporn Publisher: ISBN: 9781369615227 Category : Languages : en Pages :
Book Description
Bell pepper (Capsicum annuum) is a highly consumed vegetable in the U.S. However, it is also highly susceptible to many diseases. Phytophthora capsici is one of the major pathogens of bell pepper production. Introgression of sources of genetic resistance into commercial varieties is an effective approach for disease management. In this study, resistance loci for different isolates in a pepper recombinant inbred line (RIL) population, along with some desirable traits such as fruit size and unfavorable traits associated with the resistant parent, were identified. Quantitative trait loci (QTL) analysis identified the major components of P. capsici resistance, as well as components of resistance that were isolate-specific. QTL for organ (fruit and leaf) size clustered into a few loci in the pepper genome. The linkage map and correlation study revealed relationships among the traits. Genetic and physical genomic maps were used to investigate the candidate genes for all traits. R-gene complexes were proposed to contain the candidate genes for the resistance at the major QTL, whereas three genes on chromosomes 1, 2 and 3 were suggested for organ size. Combining the phenotypic and genotypic data from all traits, a RIL was selected for the resistant parent and crossed to bell-type varieties to develop marketable bell pepper lines containing broad genetic resistance for P. capsici.
Author: Satbir Singh Gosal Publisher: Springer Nature ISBN: 3030472981 Category : Science Languages : en Pages : 457
Book Description
Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This edited volume summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.
Author: William Zebulun Rehrig Publisher: ISBN: 9781321363784 Category : Languages : en Pages :
Book Description
A major problem for the pepper (Capsicum annuum) industry is the root and crown rot disease caused by Phytophthora capsici (Pc), to which all commercial varieties suffer yield losses despite good management practices and available landraces with high levels of resistance. Introgression of genetic resistance from pepper landraces to cultivated varieties would benefit the growers and environment through reduced pesticide applications and increased crop value. Genetic linkage maps and a physical genomic map were employed to identify resistance loci and candidate genes conferring resistance. Pepper recombinant inbred lines (RILs) have been systematically screened for Pc resistance against a set of isolates collected from Mexico, New Mexico, New Jersey, California, Michigan and Tennessee. Quantitative Trait Loci (QTL) associated with effective resistance across isolates have been identified and validated with SNP markers across additional segregating populations. RNA-Seq data were also compared in resistant and susceptible bulks to evaluate differential expression leading to resistance. By leveraging transcriptomic and genomic information, we describe several candidate genes responsible for the major QTL on chromosome P5 for resistance to Pc. SNP markers for the resistant allele were validated to facilitate gene pyramiding schemes for recurrent selection in pepper.
Author: N Manikanda Boopathi Publisher: Springer Science & Business Media ISBN: 8132209583 Category : Science Languages : en Pages : 304
Book Description
Genetic mapping and marker assisted selection (MAS) is considered as one of the major tools in genetic improvement of crop plants in this genomics era. This book describes basics in linkage mapping, step-by-step procedure to perform MAS, achievements made so far in different crops, and limitations and prospects of MAS in plant breeding. It summarizes all this in a simple but comprehensive mode using suitable examples so as to explain the concept and its historical developments. To summarize, this book describes technologies for identification of genes of interest through genetic mapping, recaps the major applications of MAS to plant breeding; lists examples in which MAS is being applied to various breeding programs, and emphasizes the various difficulties that limit the application of MAS in plant breeding, providing possible solutions to overcome these difficulties, and finally tries to illustrate the future prospects. This book would be a valuable guide to the under-graduates and post-graduates of agricultural universities and institutes that are interested and/or involved in genetic improvement of crop plants using modern tools. Bibliography listed in this book constitutes two parts: literature cited and further reading. Literature cited contains references cited in the text and further information on the given concept/technique can be obtained from these references. Further reading provides a list of suggested readings for in-depth coverage of the topics.
Author: Eleni Tani Publisher: Frontiers Media SA ISBN: 2832545696 Category : Science Languages : en Pages : 167
Book Description
Horticultural crops are an excellent source of vitamins, antioxidants, and fibers that play an important role in human health. The discovery of modern genomic tools, the continuous generation of genomic data, and the application of comparative and functional genomics are high importance innovations with numerous applications in crop breeding. The utilization of all these new techniques in combination with the analysis of genomic data using bioinformatics tools, contribute to a better understanding of the function of various agronomic traits of interest as well as horticultural crops breeding. Over the last few years, novel genome editing tools such as the CRISPR-Cas9, have been developed and revolutionized molecular technologies due to their simplicity, high efficiency, and specificity.
Author: Gregory Martin Vogel Publisher: ISBN: Category : Languages : en Pages : 0
Book Description
Phytophthora blight, caused by the oomycete pathogen Phytophthora capsici, is a devastating disease of various vegetable crops, including squash (Cucurbita pepo) and pepper (Capsicum annuum). Options for host resistance to Phytophthora blight vary dramatically between these two crops. In squash, no sources of complete resistance have been described, although variation for quantitative disease resistance exists in both commercial and unadapted germplasm. In pepper, on the other hand, landraces demonstrating complete or near-complete disease resistance have been identified and used in breeding. However, varieties with durable resistance to diverse pathogen isolates have not been developed. In order to contribute to genetic solutions for disease control in these two crops, genetic variation in squash associated with quantitative disease resistance as well as genetic variation in P. capsici associated with virulence on distinct pepper genotypes were characterized. In squash, two quantitative trait locus (QTL) mapping approaches were used in a zucchini _ gray zucchini population, resulting in the discovery of six QTL, representing the first reported for Phytophthora blight resistance in C. pepo. In order to identify genetic variants in P. capsici associated with virulence on pepper, a collection of 252 pathogen isolates, predominantly from vegetable production regions of New York state, was genotyped at over 100,000 single-nucleotide polymorphism (SNP) loci using genotyping-by-sequencing. The utility of this isolate collection as a genetic resource was demonstrated by the successful identification of SNPs associated with mating type and sensitivity to the fungicide mefenoxam via a genome-wide association study (GWAS). Inoculation of 117 genetically distinct isolates onto a panel of 16 pepper accessions revealed a significant effect of inter-species genotype-genotype interactions on disease outcomes. However, the only SNP associated with virulence in a GWAS demonstrated an effect that was consistent across pepper genotypes, suggesting that genes involved in host-specific virulence may be of smaller effect and more challenging to map. The results of these genetic experiments - conducted on both pathogen and host - are expected to inform efforts to breed vegetable crops with durable Phytophthora blight resistance.
Author: Michelle Renee Curtis
Author: Michelle Renee Curtis
Author: Jareerat Chunthawodtiporn
Author: Satbir Singh Gosal
Author: William Zebulun Rehrig
Author: N Manikanda Boopathi
Author: Wing Yee Liu
Author: Eleni Tani
Author: Mojtaba Massoudi
Author: Gregory Martin Vogel
Author: Chad Michael Jorgensen