Probes of Structure and Function of Macromolecules and Membranes: Probes and membrane function, edited by B. Chance, C. Lee, and J. K. Blasie PDF Download

Author: Britton Chance
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 592

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Author: Britton Chance
Publisher: Academic Press
ISBN: 148327148X
Category : Science
Languages : en
Pages : 582

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Book Description
Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid reactions and detection of enzymatic reactions in simple and complex systems. This book describes the application of the incisive techniques employed for the study of activity-related structural changes in enzymes to functionally related structural changes in membranes. The structural changes initiated in response to energy coupling and ion movements in mitochondria and axons are also elaborated. This publication covers the basic mechanisms by which structural changes can participate in energy coupling at the redox level of the hemoproteins that leads to the evaluation of the function of ubiquinone and cytochrome b in energy coupling. The function of factors that couple electron transport and energy conservation is likewise considered. This volume is a good reference for students and researchers conducting work on the structure and function of macromolecules and membranes.

Author: Iain Johnson
Publisher:
ISBN: 9780982927915
Category : Fluorescent probes
Languages : en
Pages : 1060

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Book Description
The most complete fluorescent labeling and detection reference available, The Molecular Probes HandbookA Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 technology solutions representing a wide range of biomolecular labeling and detection reagents. The significantly revised 11th Edition features extensive references, reorganized content, and new technical notes and product highlights.

Author: Leslie M. Loew
Publisher: CRC Press
ISBN: 1351085255
Category : Science
Languages : en
Pages : 227

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Book Description
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.

Author: Yves Mely
Publisher: Springer Science & Business Media
ISBN: 3642331289
Category : Science
Languages : en
Pages : 484

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Book Description
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Author:
Publisher: Academic Press
ISBN: 0128033274
Category : Science
Languages : en
Pages : 372

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Book Description
Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. Written by leading experts Contains original material, both textual and illustrative, that should become a very relevant reference material The material is presented in a very comprehensive manner Both researchers in the field and general readers should find relevant and up-to-date information

Author: Britton Chance
Publisher:
ISBN:
Category : Biochemistry
Languages : en
Pages : 664

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Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0

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Author: E. Anibal Disalvo
Publisher: Springer
ISBN: 3319190601
Category : Science
Languages : en
Pages : 284

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Book Description
This book is about the importance of water in determining the structure, stability and responsive behavior of biological membranes. Water confers to lipid membranes unique features in terms of surface and mechanical properties. The analysis of the hydration forces, plasticiser effects, controlled hydration, formation of microdomains of confined water suggests that water is an active constituent in a water-lipid system. The chapters describe water organization at the lipid membrane–water interphase, the water penetration, the long range water structure in the presence of lipid membranes by means of X-ray and neutron scattering, general polarization, fluorescent probes, ATR-FTIR and near infrared spectroscopies, piezo electric methods, computer simulation and surface thermodynamics. Permeation, percolation, osmotic stress, polarization, protrusion, sorption, hydrophobicity, density fluctuations are treated in detail in self-assembled bilayers. Studies in lipid monolayers show the correlation of surface pressure with water activity and its role in peptide and enzyme interactions. The book concludes with a discussion on anhydrobiosis and the effect of water replacement in microdomains and its consequence for cell function. New definitions of lipid/water interphases consider water not only as a structural-making solvent but as a mediator in signalling metabolic activity, modulating protein insertion and enzymatic activity, triggering oscillatory reactions and functioning of membrane bound receptors. Since these effects occur at the molecular level, membrane hydration appears fundamental to understand the behavior of nano systems and confined environments mimicking biological systems. These insights in structural, thermodynamical and mechanical water properties give a base for new paradigms in membrane structure and function for those interested in biophysics, physical chemistry, biology, bio and nano medicine, biochemistry, biotechnology and nano sciences searching for biotechnological inputs in human health, food industry, plant growing and energy conversion.

Author: Alex M. Dopico
Publisher: Springer Science & Business Media
ISBN: 1588296628
Category : Medical
Languages : en
Pages : 1265

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Book Description
This book presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. The volume provides a comprehensive set of techniques for studying membrane lipids with a strong biophysical emphasis. It compares the various available techniques including the pros and cons as seen by the experts.