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Author: Michael Esseling Publisher: Springer ISBN: 3319093185 Category : Science Languages : en Pages : 133
Book Description
In the never-ending quest for miniaturization, optically controlled particle trapping has opened up new possibilities for handling microscopic matter non-invasively. This thesis presents the application of photorefractive crystals as active substrate materials for optoelectronic tweezers. In these tweezers, flexible optical patterns are transformed into electrical forces by a photoconductive material, making it possible to handle matter with very high forces and high throughput. Potential substrate materials’ properties are investigated and ways to tune their figures-of-merit are demonstrated. A large part of the thesis is devoted to potential applications in the field of optofluidics, where photorefractive optoelectronic tweezers are used to trap, sort and guide droplets or particles in microfluidic channels, or to shape liquid polymers into optical elements prior to their solidification. Furthermore, a new surface discharge model is employed to discuss the experimental conditions needed for photorefractive optoelectronic tweezers.
Author: Michael Esseling Publisher: Springer ISBN: 3319093185 Category : Science Languages : en Pages : 133
Book Description
In the never-ending quest for miniaturization, optically controlled particle trapping has opened up new possibilities for handling microscopic matter non-invasively. This thesis presents the application of photorefractive crystals as active substrate materials for optoelectronic tweezers. In these tweezers, flexible optical patterns are transformed into electrical forces by a photoconductive material, making it possible to handle matter with very high forces and high throughput. Potential substrate materials’ properties are investigated and ways to tune their figures-of-merit are demonstrated. A large part of the thesis is devoted to potential applications in the field of optofluidics, where photorefractive optoelectronic tweezers are used to trap, sort and guide droplets or particles in microfluidic channels, or to shape liquid polymers into optical elements prior to their solidification. Furthermore, a new surface discharge model is employed to discuss the experimental conditions needed for photorefractive optoelectronic tweezers.
Author: Ki Young Kim Publisher: IntechOpen ISBN: 9789537619718 Category : Technology & Engineering Languages : en Pages : 468
Book Description
Research and development in modern optical and photonic technologies have witnessed quite fast growing advancements in various fundamental and application areas due to availability of novel fabrication and measurement techniques, advanced numerical simulation tools and methods, as well as due to the increasing practical demands. The recent advancements have also been accompanied by the appearance of various interdisciplinary topics. The book attempts to put together state-of-the-art research and development in optical and photonic technologies. It consists of 21 chapters that focus on interesting four topics of photonic crystals (first 5 chapters), THz techniques and applications (next 7 chapters), nanoscale optical techniques and applications (next 5 chapters), and optical trapping and manipulation (last 4 chapters), in which a fundamental theory, numerical simulation techniques, measurement techniques and methods, and various application examples are considered. This book deals with recent and advanced research results and comprehensive reviews on optical and photonic technologies covering the aforementioned topics. I believe that the advanced techniques and research described here may also be applicable to other contemporary research areas in optical and photonic technologies. Thus, I hope the readers will be inspired to start or to improve further their own research and technologies and to expand potential applications. I would like to express my sincere gratitude to all the authors for their outstanding contributions to this book.
Author: Eileen Otte Publisher: Springer Nature ISBN: 3030637158 Category : Science Languages : en Pages : 184
Book Description
Structured singular light is an ubiquitous phenomenon. It is not only created when light refracts at a water surface but can also be found in the blue daytime sky. Such light fields include a spatially varying amplitude, phase, or polarization, enabling the occurrence of optical singularities. As structurally stable units of the light field, these singularities are particularly interesting since they determine its topology. In this excellent book, the author presents a pioneering study of structured singular light, thereby contributing many original approaches. Especially in the field of polarization and its rich number of different types of singularities the book defines and drives a completely new field. The work demonstrates how to control complex polarization singularity networks and their propagation. Additionally, the author pioneers tightly focusing vectorial beams, also developing an urgently needed detection scheme for three-dimensional nanoscale polarization structures. She also studies classical spatial entanglement using structured light, introducing entanglement beating and paraxial spin-orbit-coupling. The book is hallmarked by its comprehensive and thorough way of describing a plethora of different approaches to structure light by amplitude, phase and polarization, as well as the important role of optical singularities.
Author: Arne Gennerich Publisher: Springer Nature ISBN: 1071622293 Category : Science Languages : en Pages : 753
Book Description
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Author: Matthew A. Cibula Publisher: ISBN: Category : Collagen Languages : en Pages : 107
Book Description
The development and some applications of holographic optical tweezers (HOT) are presented. Our HOT system uses a spatial light modulator (SLM) to control the location and properties of the optical trap. We have developed a method for optimizing the diffraction efficiency of a SLM that can be applied in situ and addresses the issues of nonlinear phase modulation and phase modulation less than 2[pi]. The method employs a one-dimensional blazed phase grating written on the SLM. For an ideal SLM, the phase shift is linear and covers 0-2[pi], yielding a first-order diffraction efficiency of unity. For a realistic SLM with nonlinear or reduced phase shift, the efficiency is approximately [eta] =1 - [sigma]2, where [sigma]2 is the variance of the phase error from the ideal case. Because each pixel contributes to the phase error independently, this suggests a method to maximize the efficiency by adjusting the phase encoding of the SLM pixel-by-pixel. In practice, we do this by adjusting the gray-scale of each pixel while measuring the first-order diffracted power. The collection of optimal gray values comprises the optimized gray-scale lookup table, which exhibits the nonlinearity required to produce a linear phase grating and the saturated phase encoding that maximizes the efficiency of phase limited SLMs. The optimized SLM enables strong trapping power, even when distributed among multiple traps, which is essential to enable our system to trap multiple nanosensors and simultaneously detect the sensors' fluorescence spectra with an imaging spectrometer. Such nanosensors are capable of detecting changes in their environment such as pH, ion concentration, temperature, and voltage by monitoring changes in the nanosensors' emitted fluorescence spectra. We have used streptavidin labeled quantum dots bound to the surface of biotin labeled polystyrene microspheres to measure temperature changes by observing a corresponding shift in the wavelength of the spectral peak, which is excited with a 532 nm wide field laser source. Particles with diameter greater than the wavelength of light exhibit Mie resonances in their fluorescence spectrum whose spectral locations are dependent on the size of the particle and the relative index of refraction between the particle and the surrounding medium. HOT also provides a useful platform to study the micromechanical properties of elastic materials such as collagen. Collagen gels are widely used in experiments on cell mechanics because collagen is the most abundant protein in the mammalian extracellular matrix and is the primary source of its mechanical properties. Collagen gels are often approximated as homogeneous elastic materials; however, variations in the collagen fiber microstructure and cell adhesion forces cause the mechanical propertiesto be inhomogeneous at the cellular scale. We study the mechanics of type I collagen on the scale of tens to hundreds of microns by using HOT to apply picoNewton forces to micron-sized particles embedded in the collagen fiber network. We measure the local compliance and elastic modulus of the collagen network and find that particle displacements are inhomogeneous, anisotropic and asymmetric. Confocal reflection microscopy is used to reveal the local fiber structure and a simulation treating the network as a triangular lattice is used for comparison to the HOT measurements. Collagen samples prepared at 21°C and 37°C show that gels formed at lower temperature are more inhomogeneous, anisotropic, and compliant than those formed at high temperature, and cellularized samples allow us to characterize the effects of cell adhesion forces on the network mechanics.
Author: Michael Esseling
Author: Michael Esseling
Author: Ki Young Kim
Author: Eileen Otte
Author: Arne Gennerich
Author: 
Author: Rory M. Power
Author: Matteo Mazzanti
Author: 
Author: Matthew A. Cibula
Author: