PCR Guru PDF Download

Author: Ayaz Najafov
Publisher: Academic Press
ISBN: 012804246X
Category : Science
Languages : en
Pages : 108

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Book Description
PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique “Good Practices and Tips that are indispensable for the beginner and expert alike Features “Special Cases with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem

Author: Elizabeth van Pelt-Verkuil
Publisher: Springer Science & Business Media
ISBN: 1402062419
Category : Science
Languages : en
Pages : 333

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Book Description
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Author: Arndt Rolfs
Publisher: Springer Science & Business Media
ISBN: 3642759246
Category : Medical
Languages : en
Pages : 258

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Book Description
PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Author: John M. S. Bartlett
Publisher: Springer Science & Business Media
ISBN: 1592593844
Category : Science
Languages : en
Pages : 1083

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Book Description
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Author: Alexzander A. A. Asea
Publisher: Springer Nature
ISBN: 3030851133
Category : Medical
Languages : en
Pages : 242

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Book Description
The book Coronavirus Therapeutics Volume II provides the most comprehensive review on contemporary knowledge on the consequences during the outbreak of Coronavirus. This also describes the clinical features, diagnostic evaluation and management of Coronavirus patients. Using an integrative approach to the understanding of paradigm gap in host-pathogen interaction studies to the management of Coronavirus patients in the Intensive Care Unit to the current challenges for the effective management of Coronavirus pandemic risk factors including diabetes, hypertension, obesity and repercussion on the central and peripheral nervous system. Key basic and clinical research laboratories from major universities, academic medical hospitals, biotechnology and pharmaceutical laboratories around the world have contributed articles that review present research activity on the role of heat shock proteins in human diseases and importantly project the field into the future. The book is a must read for graduate students. medical students, basic science researchers and postdoctoral scholars in the fields of Cancer Biology, Oncology, Cardiovascular Diseases, Microbiology, Inflammation, Translational Medicine, Clinical Research, Biotechnology, Cell & Molecular Medicine, Pharmaceutical Scientists and Researchers involved in Drug Discovery.

Author: Lucília Domingues
Publisher: Springer Nature
ISBN: 1071633589
Category : Science
Languages : en
Pages : 255

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Book Description
This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author: Michael A. Innis
Publisher: Elsevier
ISBN: 0080538541
Category : Science
Languages : en
Pages : 391

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Book Description
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

Author: Sarah Maddocks
Publisher: Academic Press
ISBN: 0128026979
Category : Science
Languages : en
Pages : 100

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Book Description
Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Author: Paul Rabinow
Publisher: University of Chicago Press
ISBN: 022621687X
Category : Science
Languages : en
Pages : 200

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Book Description
Making PCR is the fascinating, behind-the-scenes account of the invention of one of the most significant biotech discoveries in our time—the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a given segment in a short period of time. It makes abundant what was once scarce—the genetic material required for experimentation. Making PCR explores the culture of biotechnology as it emerged at Certus Corporation during the 1980s and focuses on its distinctive configuration of scientific, technical, social, economic, political, and legal elements, each of which had its own separate trajectory over the preceding decade. The book contains interviews with the remarkable cast of characters who made PCR, including Kary Mullin, the maverick who received the Nobel prize for "discovering" it, as well as the team of young scientists and the company's business leaders. This book shows how a contingently assembled practice emerged, composed of distinctive subjects, the site where they worked, and the object they invented. "Paul Rabinow paints a . . . picture of the process of discovery in Making PCR: A Story of Biotechnology [and] teases out every possible detail. . . . Makes for an intriguing read that raises many questions about our understanding of the twisting process of discovery itself."—David Bradley, New Scientist "Rabinow's book belongs to a burgeoning genre: ethnographic studies of what scientists actually do in the lab. . . . A bold move."—Daniel Zalewski, Lingua Franca "[Making PCR is] exotic territory, biomedical research, explored. . . . Rabinow describes a dance: the immigration and repatriation of scientists to and from the academic and business worlds."—Nancy Maull, New York Times Book Review

Author: Mike McPherson
Publisher: Garland Science
ISBN: 0203002679
Category : Science
Languages : en
Pages : 292

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Book Description
A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.