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Author: Helen H. Lee Publisher: Springer Science & Business Media ISBN: 9780817639211 Category : Medical Languages : en Pages : 300
Book Description
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this book goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods including the Ligase Chain Reaction, Q[beta] Replicase Assays and TMA. There are many examples of specific applications of these technologies, discussions of yet unresolved issues and demonstrations of the relevance of these technologies to medical research and disease diagnostics. Individual chapters cover uses of these methods in clinical situations such as detection of food pathogens, viral infections, STDs, Mycobacteria drug resistance mutations, and heritable diseases. Automation, diagnostic test evaluation, and the synthesis of artificial DNA are also discussed. This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis.
Author: Helen H. Lee Publisher: Springer Science & Business Media ISBN: 9780817639211 Category : Medical Languages : en Pages : 300
Book Description
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this book goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods including the Ligase Chain Reaction, Q[beta] Replicase Assays and TMA. There are many examples of specific applications of these technologies, discussions of yet unresolved issues and demonstrations of the relevance of these technologies to medical research and disease diagnostics. Individual chapters cover uses of these methods in clinical situations such as detection of food pathogens, viral infections, STDs, Mycobacteria drug resistance mutations, and heritable diseases. Automation, diagnostic test evaluation, and the synthesis of artificial DNA are also discussed. This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis.
Author: Gary A. Toranzos Publisher: CRC Press ISBN: 9781566764087 Category : Science Languages : en Pages : 240
Book Description
From the Preface Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been used successfully for a variety of samples, but this method was still not sensitive enough. The next logical step was the application of the recently developed DNA amplification technique known as the polymerase chain reaction, or PCR. Since then, many laboratories around the world have adopted PCR for environmental work. Samples obtained from soils, water and air are enormously complex because they are unknown mixtures of DNA and other compounds. Thus, procedures for target DNA amplification from the environment require special attention. The PCR has allowed us to go beyond the need for culturing prior to analysis of microbial communities. It has been shown that even microorganisms that can be routinely grown in the laboratory undergo some physiological changes when exposed to the environment. One of these changes (first observed by R. Colwell and colleagues) is known as the viable-but-non-culturable state, and seems to be a common occurrence. Thus, the use of culture techniques paint only part of the picture in terms of microbial behavior under environmental conditions. The ability to amplify nucleic acids by the PCR has brought about a myriad of very ingenious modifications to the technique that can then be used to study complex ecosystems. The manner in which the PCR can be modified is only limited by the need and/or the imagination of the researcher. The first manual dedicated specifically to the analysis (by PCR) of environmental samples, Environmental Applications of Nucleic Acid Amplification Techniques presents state of the art methodology for the detection of microorganisms in soil, water, air samples, as well as the amplification of nucleic acids from fossil samples. The manual gives step-by-step procedures for the analysis of these samples. Although several publications have addressed the use of Polymerase Chain Reaction technique, very few of them have been directed toward the application of this technique to environmental samples. This book fills this gap in the literature.
Author: Chang-Hui Shen Publisher: Elsevier ISBN: 0323986099 Category : Science Languages : en Pages : 590
Book Description
Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. Provides explanations on how techniques are used to diagnosis at the molecular level Explains how to use information technology to communicate and assess results in the lab Enhances our understanding of fundamental molecular biology and places techniques in context Places protocols into context with practical applications Includes extra chapters on respiratory viruses (Corona)
Author: Vadim V. Demidov Publisher: Taylor & Francis ISBN: 9780954523299 Category : Science Languages : en Pages : 342
Book Description
Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Author: Yi-Wei Tang Publisher: Springer Science & Business Media ISBN: 0387328920 Category : Medical Languages : en Pages : 550
Book Description
Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.
Author: Patricia Frye Walker Publisher: Elsevier Health Sciences ISBN: 0323070574 Category : Medical Languages : en Pages : 783
Book Description
Immigrant Medicine is the first comprehensive guide to caring for immigrant and refugee patient populations. Edited by two of the best-known contributors to the growing canon of information about immigrant medicine, and written by a geographically diverse collection of experts, this book synthesizes the most practical and clinically relevant information and presents it in an easy-to-access format. An invaluable resource for front-line clinicians and other healthcare professionals, public health officials, and policy makers, Immigrant Medicine is destined to become the benchmark reference in this emerging field. Features expert guidance on data collection, legal, interpretive and social adjustment issues, as well as best practices in caring for immigrants to help you confidently manage all aspects of immigrant medicine. Includes detailed discussions on major depression, post traumatic stress disorder, and issues related to torture so you can effectively diagnose and treat common psychiatric issues. Covers international and new-arrival screening and immunizations offering you invaluable advice. Presents a templated diseases/disorders section with discussions on tuberculosis, hepatitis B, and common parasites that helps you easily manage the diseases and syndromes you are likely to encounter. Provides boxed features and tables, differential diagnoses, and treatment algorithms to help you absorb information at a glance.
Author: Helen H. Lee
Author: Helen H. Lee
Author: Helen Lee
Author: Gary A. Toranzos
Author: Australian Health Technology Advisory Committee
Author: Chang-Hui Shen
Author: Vadim V. Demidov
Author: Kirstin J. Edwards
Author: 
Author: Yi-Wei Tang
Author: Patricia Frye Walker