Author: Society of General PhysiologistsPublisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
New Optical Methods in Cell Physiology
Author: Society of General PhysiologistsPublisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
Optical Methods in Cell Physiology
Author: Paul De WeerPublisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 506
Book Description
A book for physiologists, biologists and biochemists studying cell physiology. Included are major optical techniques and bases for designing experiments and choosing appropriate instrumentation, along with discussions on methods and results of optical techniques applied to research through image enhancement, probes for membrane potential, intracellular indicators for Ca and pH, and photobleaching and photoactivation techniques. Also included are schematic drawings and numerous references.
New Optical Methods in Cell Physiology
Author: Graham Ellis-DaviesNew Optical Methods in Cell Physiology
Author: New Optical Methods in Cell Physiology
Author: Brian M. SalzbergModern Optics, Electronics and High Precision Techniques in Cell Biology
Author: Gerhard IsenbergPublisher: Springer Science & Business Media
ISBN: 3642803709
Category : Science
Languages : en
Pages : 253
Book Description
In spite of tremendous scientific progress over the past years, cell biologists do not yet understand the fundamental processes that determine the life cy cle of a cell. Such are: cell movement and cell spreading, cell division, cell communication, cell signaling, cell regeneration and cell death. Biochemistry has enabled us to recognize and to isolate an overwhelming number of new proteins. In vitro assays and the reinjection of proteins into cells and tissues have provided insights into molecular functions and cellular mechanisms. The renaissance of the genetic approach by applying restriction enzymes and vectors, PCR and antisense technology has enabled us to overexpress certain cellular products, to make altered constructs of cell components or to create "knock-out" mutants that entirely lack the factor of interest. Amazingly en ough, all these molecular toys have led to a stream of information but not, in a comparable degree, to a better understanding. Has the puzzle become too complex to get solved; or are the windows too small that we are looking through? As an attempt to answer both questions, the aim of the present mono graph Modern Optics, Electronics and High Precision Techniques in Cell Biol ogy is first to provide cell and molecular biologists with a whole new scope of easily applicable techniques including brand-new optical, biophysical, physicochemical and biosensoric devices. Secondly, these newly developed techniques allow us to look at cells and biological systems as a whole.
Digital Microscopy
Author: Greenfield SluderPublisher: Elsevier
ISBN: 0080544347
Category : Science
Languages : en
Pages : 626
Book Description
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
Optical Imaging Techniques in Cell Biology, Second Edition
Author: Guy CoxPublisher: CRC Press
ISBN: 1439848254
Category : Medical
Languages : en
Pages : 319
Book Description
Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.
Optical Imaging Techniques in Cell Biology
Author: Guy CoxPublisher: CRC Press
ISBN: 1420005618
Category : Science
Languages : en
Pages : 304
Book Description
Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E
Optical Microscopy
Author: Brian HermanPublisher: Elsevier
ISBN: 0080571395
Category : Science
Languages : en
Pages : 462
Book Description
Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances Caged compounds, fluorescence ratio imaging, and CCD video cameras Simultaneous multiple detection and real-time fluorescence microscopy Simultaneous DIC and quantitative LLF video imaging Total internal reflectance, time-resolved, and automated fluorescence microscopy Laser-scanning confocal microscopy Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells