Multifunctional Molecular Recognition of DNA/RNA Secondary Structure by Molecular Senzors PDF Download

Author: Tamara Šmidlehner
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Keith R Fox
Publisher: Royal Society of Chemistry
ISBN: 1849734933
Category : Science
Languages : en
Pages : 317

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Book Description
Applications of nucleic acids have developed recently to provide solutions for biosensors, diagnostic tools and as platforms for the assembly of complex structures. These developments have been possible as their base sequence can be used to assemble precise structures following simple and predictable rules. Self-assembled DNA can then be amplified using polymerase chain reaction (PCR) and this ultimately enables the preparation of synthetic nucleic acids. Their use as molecular tools or DNA-conjugates has recently been enhanced by the addition of other groups including enzymes, fluorophores and small molecules. Written by leaders in the field, this volume describes the preparation and application of these DNA-conjugates. Several have been used as sensors (aptamers, riboswitches and nanostructures) based on the ability of nucleic acids to adopt specific structures in the presence of ligands, whilst others link reporter groups such as proteins or fluorophores to RNA or DNA for detection, single molecule studies, and increasing the sensitivity of PCR. The book is relevant to researchers in areas related to analytical chemistry, chemical biology, medicinal chemistry, molecular pharmacology, and structural and molecular biology.

Author: David Elliott
Publisher: Oxford University Press
ISBN: 019107103X
Category : Science
Languages : en
Pages : 445

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RNA plays a central, and until recently, somewhat underestimated role in the genetics underlying all forms of life on earth. This versatile molecule not only plays a crucial part in the synthesis of proteins from a DNA template, but is also intrinsically involved in the regulation of gene expression, and can even act as a catalyst in the form of a ribozyme. This latter property has led to the hypothesis that RNA - rather than DNA - could have played an essential part in the origin of life itself. This landmark text provides a systematic overview of the exciting and rapidly moving field of RNA biology. Key pioneering experiments, which provided the underlying evidence for what we now know, are described throughout, while the relevance of the subject to human disease is highlighted via frequent boxes. For the second edition of Molecular Biology of RNA, more introductory material has been incorporated at the beginning of the text, to aid students studying the subject for the first time. Throughout the text, new material has been included - particularly in relation to RNA binding domains, non-coding RNAs, and the connection between RNA biology and epigenetics. Finally, a new closing chapter discusses how exciting new technologies are being used to explore current topical areas of research.

Author: Alba Monferrer i Sureda
Publisher:
ISBN:
Category :
Languages : en
Pages : 85

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DNA and RNA have emerged as a material for nanotechnology applications that capitalize on the nucleic acids' ability to encode folding and programmable self-assembly through mainly base pairing. While DNA and RNA offer distinct advantages as components of nano-architectures, the two types of nucleic acids have rarely been used in combination to enhance structural diversity or for partitioning of functional and architectural roles. We have used a combined design and screening strategy to discover combinations of RNA motifs as architectural joints and DNA building blocks as functional modules that allow the self-assembly of a versatile toolkit of nucleic acid nanoshapes. The design and screening approach enables the systematic development of hybrid RNA/DNA nanoshapes as robust programmable platforms for applications in molecular recognition, sensor and catalyst development as well as protein interaction studies.

Author: Scott K. Silverman
Publisher: Springer Science & Business Media
ISBN: 0387472576
Category : Medical
Languages : en
Pages : 131

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This book reviews studies showing that interesting and useful molecular-scale switches and sensors can be made out of nucleic acids, by both artificial and natural means. The first section of the book is devoted to artificial nucleic acid switches and sensors. In second section, three chapters show that nature has been as crafty a molecular-scale engineer as any modern scientist, in the evolution of natural nucleic acid switches and sensors.

Author: Mark A. Mentzer
Publisher: CRC Press
ISBN: 143982908X
Category : Medical
Languages : en
Pages : 368

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How does the field of optical engineering impact biotechnology? Perhaps for the first time, Applied Optics Fundamentals and Device Applications: Nano, MOEMS, and Biotechnology answers that question directly by integrating coverage of the many disciplines and applications involved in optical engineering, and then examining their applications in nanobiotechnology. Written by a senior U.S. Army research scientist and pioneer in the field of optical engineering, this book addresses the exponential growth in materials, applications, and cross-functional relevance of the many convergent disciplines making optical engineering possible, including nanotechnology, MEMS, (MOEMS), and biotechnology. Integrates Coverage of MOEMS, Optics, and Nanobiotechnology—and Their Market Applications Providing an unprecedented interdisciplinary perspective of optics technology, this book describes everything from core principles and fundamental relationships, to emerging technologies and practical application of devices and systems—including fiber-optic sensors, integrated and electro-optics, and specialized military applications. The author places special emphasis on: Fiber sensor systems Electro-optics and acousto-optics Optical computing and signal processing Optical device performance Thin film magnetic memory MEMS, MOEMS, nano- and bionanotechnologies Optical diagnostics and imaging Integrated optics Design constraints for materials, manufacturing, and application space Bridging the technology gaps between interrelated fields, this reference is a powerful tool for students, engineers and scientists in the electrical, chemical, mechanical, biological, aerospace, materials, and optics fields. Its value also extends to applied physicists and professionals interested in the relationships between emerging technologies and cross-disciplinary opportunities. Author Mark A. Mentzer is a pioneer in the field of optical engineering. He is a senior research scientist at the U.S. Army Research Laboratory in Maryland. Much of his current work involves extending the fields of optical engineering and solid state physics into the realm of biochemistry and molecular biology, as well as structured research in biophotonics.

Author: Günter Mayer
Publisher: John Wiley & Sons
ISBN: 1119957265
Category : Science
Languages : en
Pages : 595

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With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include: chemical synthesis of modified RNA expansion of the genetic alphabet in nucleic acids by creating new base pairs chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides nucleic-acid-templated chemistry chemical biology of peptide nucleic acids (PNA) the interactions of small molecules with DNA and RNA the architectural modules of folded RNAs genesis and biological applications of locked nucleic acid (LNA) small non-coding RNA in bacteria microRNA-guided gene silencing nucleic acids based therapies innate immune recognition of nucleic acid light-responsive nucleic acids for the spatiotemporal control of biological processes DNA methylation frameworks for programming RNA devices RNA as a catalyst: The Diels-Alderase-Ribozyme evolving an understanding of RNA function by in vitro approaches the chemical biology of aptamers: synthesis and applications nucleic acids as detection tools bacterial riboswitch discovery and analysis The Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.

Author: Salvador Alegret
Publisher: Elsevier
ISBN: 0080560695
Category : Science
Languages : en
Pages : 1416

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Electrochemical Sensor Analysis (ECSA) presents the recent advances in electrochemical (bio)sensors and their practical applications in real clinical, environment, food and industry related samples, as well as in the safety and security arena. In a single source, it covers the entire field of electrochemical (bio)sensor designs and characterizations. The 38 chapters are grouped in seven sections: 1) Potentiometric sensors, 2) Voltammetric sensors, 3) Electrochemical gas sensors 4) Enzyme-based sensors 5) Affinity biosensors 6) Thick and thin film biosensors and 7) Novel trends. Written by experts working in the diverse technological and scientific fields related to electrochemical sensors, each section provides an overview of a specific class of electrochemical sensors and their applications. This interdisciplinary text will be useful for researchers and professionals alike. Covers applications and problem solving (sensitivity, interferences) in real sample analysis Details procedures to construct and characterize electrochemical (bio)sensors

Author:
Publisher: World Scientific
ISBN: 9811245916
Category : Science
Languages : en
Pages : 1446

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Water is an indispensable resource for our society. Essential to sustaining life and economic prosperity, water is also the basic component for manufacturing almost everything to keep society alive, including energy, food, clothing, cars, and electronics, among many other examples. It is, thus, an integral part of our lives beyond simply quenching our thirst. In addition, our future economy and security highly depend upon the availability of clean water. Yet given its critical importance, there is a limited supply of renewable freshwater across the globe and there is no substitute. Global population and economic growth, urbanization, and climate change further exacerbate the increasing stress on freshwater supplies. As such, society urgently needs to find the scientific and engineering solutions to more efficiently manage our precious water resources. The volumes of this multi-volume reference cover the latest scientific advancements and solutions in managing and treating this crucial resource.Related Link(s)

Author: Chaoyong James Yang
Publisher: Springer Science & Business Media
ISBN: 3642391095
Category : Science
Languages : en
Pages : 201

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Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.