Multibeam Multifocal Multiphoton Photon Counting Imaging in Scattering Media PDF Download

Author: Erich E. Hoover
Publisher:
ISBN:
Category : Multiphoton excitation microscopy
Languages : en
Pages :

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Author: Adrià Escobet Montalbán
Publisher:
ISBN:
Category : Fluorescence microscopy
Languages : en
Pages : 0

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Author: Nikolay Britun
Publisher: BoD – Books on Demand
ISBN: 953513907X
Category : Science
Languages : en
Pages : 298

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Photon counting is a unified name for the techniques using single-photon detection for accumulative measurements of the light flux, normally occurring under extremely low-light conditions. Nowadays, this approach can be applied to the wide variety of the radiation wavelengths, starting from X-ray and deep ultraviolet transitions and ending with far-infrared part of the spectrum. As a special tribute to the photon counting, the studies of cosmic microwave background radiation in astronomy, the experiments with muon detection, and the large-scale fundamental experiments on the nature of matter should be noted. The book provides readers with an overview on the fundamentals and state-of-the-art applications of photon counting technique in the applied science and everyday life.

Author: Peter Kapusta
Publisher: Springer
ISBN: 3319156365
Category : Science
Languages : en
Pages : 371

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This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Author: Aaron Mok
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Imaging large populations of neurons across multiple brain regions at substantial depth are necessary to understand animal behaviors. However, this is challenging for optical imaging due to: (a) optical aberrations, scattering, and absorption in biological tissue, and (b) the number of neurons that can be recorded is limited by the "photon budget"-under a maximum allowable average power on the biological sample, only a limited number of neurons can be imaged. This limits both imaging depth and imaging width in multiphoton imaging.In this thesis, we first report a simple and versatile tissue spectrometer to measure the optical scattering and absorption in biological samples in terms of ballistic and total transmittance at a wavelength from 450 nm to 1630 nm. The measurement results are important to determine the optimal excitation wave length and fluorophores for deep imaging. With measurements showing that fly head cuticle has high transmission at wavelengths of > 900 nm, we were able to develop a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticles. This through-cuticle imaging method extends the time limits of in-vivo imaging in flies and opens new avenues for imaging the neuronal structure and activity of an intact fly brain. Challenges posed by the photon budget for deep and wide imaging in multiphoton imaging can be partially mitigated by careful optimization of the excitation wavelength, excitation focus profile, and allocation of laser power. We carefully compared the excitation efficiency of Gaussian focus, variations of Gaussian focus, and Bessel focus. We found that for neuronal activity imaging in three-photon microscopy, using multiple foci on the same neuron with an enlarged Gaussian focus of _ 5 - 10 μm axial extension provides the best spike detection accuracy. Finally, we developed a synergistic combination of using multiple foci and adaptive excitation to reduce the average power required on the sample through the intelligent allocation of laser power only on the regions of interest. These optimizations lead to a more efficient imaging scheme in three-photon excitation. It enabled us to improve the efficiency, scanning speed, and field-of-view of three-photon microscopy without exceeding the allowable power limit. We built a large field-of-view multiphoton microscope for two- and three-photon imaging and demonstrated three-photon imaging with a field-of-view of _ 3.5 mm in diameter and > 1 mm in depth across various mouse brain regions and across the entire width of the zebrafish brain.

Author: Ulrich Kubitscheck
Publisher: John Wiley & Sons
ISBN: 3527687726
Category : Medical
Languages : de
Pages : 508

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Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Author: Josef F. Bille
Publisher: Springer
ISBN: 3030166384
Category : Medical
Languages : en
Pages : 411

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This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Author: Francesco S. Pavone
Publisher: CRC Press
ISBN: 1498718760
Category : Medical
Languages : en
Pages : 579

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The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light. Including 32 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain. Key Features: Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels. Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice. Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging. Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.

Author:
Publisher: Frontiers Media SA
ISBN: 2889712524
Category : Science
Languages : en
Pages : 156

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Author: Wolfgang Becker
Publisher: Springer Science & Business Media
ISBN: 3540288821
Category : Science
Languages : en
Pages : 414

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In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.