Mitochondria Sharing and Cell-Signaling Dynamics in Human-Adipose Derived Stem Cells PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Mitochondria Sharing and Cell-Signaling Dynamics in Human-Adipose Derived Stem Cells PDF full book. Access full book title Mitochondria Sharing and Cell-Signaling Dynamics in Human-Adipose Derived Stem Cells by Shalise Anna Burch. Download full books in PDF and EPUB format.
Author: Shalise Anna Burch Publisher: ISBN: Category : Languages : en Pages :
Book Description
Stem cells are widely known for their potential to transform into various cell types. Less known is their remarkable ability to rescue cells and promote tissue growth through mitochondria sharing. Healthy mitochondria are crucial for cell proliferation, without properly functioning mitochondria cells become dysfunctional, which may result in disease or cell death. Damage in mitochondrial DNA (mtDNA) causes a wide range of highly crippling and often-fatal disorders. Transfer of stem cell mitochondria to injured cells has been shown to rescue oxidative phosphorylation as well to promote tissue growth and repair. The unique regenerative abilities of stem cells offer great potential for treating diseases. However, in the presence of tumor cells, mitochondria sharing could enhance energy production and accelerate tumor formation. The main goal of our research was to better understand the dynamics and regulatory mechanisms involved during stem cell mitochondria transfer. We had two specific aims: Aim 1) To measure the optimal parameters that induce mitochondria transfer among stem cells. These parameters allowed us to explore the effects of mitochondria sharing between stem cells and cancer cells. Aim 2) To understand the dynamics of communication between stem cells and cancer cells. Our results unveil important parameters when studying mitochondrial transfer. First, mitochondrial transfer is density dependent and is also dependent on the distance between donor cell and recipient cell. Second, mitochondrial transfer is different between two-dimensional and three-dimensional microenvironments. These results must be taken into important consideration when designing experiments and applications that involve stem cell extracellular vesicles and mitochondrial transfer. Co-culture of stem cells with HEK tumorigenic cells led to some very interesting results. We observed mitochondrial transfer from stem cells to HEK, but almost no transfer was observed the other way around. We also observed a significant increase in the migratory ability of HEK cells when co-cultured with our adipose-derived stem cells. As more researchers find co-localization of stem cells and cancer cells in the human body, these results could be used to understand their biological relationship and could lead to future therapeutic applications.
Author: Shalise Anna Burch Publisher: ISBN: Category : Languages : en Pages :
Book Description
Stem cells are widely known for their potential to transform into various cell types. Less known is their remarkable ability to rescue cells and promote tissue growth through mitochondria sharing. Healthy mitochondria are crucial for cell proliferation, without properly functioning mitochondria cells become dysfunctional, which may result in disease or cell death. Damage in mitochondrial DNA (mtDNA) causes a wide range of highly crippling and often-fatal disorders. Transfer of stem cell mitochondria to injured cells has been shown to rescue oxidative phosphorylation as well to promote tissue growth and repair. The unique regenerative abilities of stem cells offer great potential for treating diseases. However, in the presence of tumor cells, mitochondria sharing could enhance energy production and accelerate tumor formation. The main goal of our research was to better understand the dynamics and regulatory mechanisms involved during stem cell mitochondria transfer. We had two specific aims: Aim 1) To measure the optimal parameters that induce mitochondria transfer among stem cells. These parameters allowed us to explore the effects of mitochondria sharing between stem cells and cancer cells. Aim 2) To understand the dynamics of communication between stem cells and cancer cells. Our results unveil important parameters when studying mitochondrial transfer. First, mitochondrial transfer is density dependent and is also dependent on the distance between donor cell and recipient cell. Second, mitochondrial transfer is different between two-dimensional and three-dimensional microenvironments. These results must be taken into important consideration when designing experiments and applications that involve stem cell extracellular vesicles and mitochondrial transfer. Co-culture of stem cells with HEK tumorigenic cells led to some very interesting results. We observed mitochondrial transfer from stem cells to HEK, but almost no transfer was observed the other way around. We also observed a significant increase in the migratory ability of HEK cells when co-cultured with our adipose-derived stem cells. As more researchers find co-localization of stem cells and cancer cells in the human body, these results could be used to understand their biological relationship and could lead to future therapeutic applications.
Author: Joana Vieira Silva Publisher: Springer Nature ISBN: 3030444368 Category : Medical Languages : en Pages : 444
Book Description
Signal transduction comprises the intracellular biochemical signals which induce the appropriate cell response to an external stimulus. The players in signal transduction are diverse, from small molecules as first messengers, to proteins, receptors, transcription factors, among many others. The different signaling pathways and the crosstalk between them originates the unique signaling profile of every cell type in the human body. The cell signaling specificity depends on several aspects including protein composition, subcellular localization and complexes and gene promoters. This textbook provides a comprehensive overview of the specific signaling pathways on a variety of human tissues. This information can be of great value for health science researchers, professionals and students to understand key pathways for tissue-specific functions in the plethora of signals, signals receptors, transducers and effectors. Chapter 3 and 15 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: Navdeep Singh Chandel Publisher: ISBN: 9781621821298 Category : SCIENCE Languages : en Pages : 0
Book Description
"Metabolic pathways used to be "road maps" most biologists learned as undergraduates and then promptly forgot. Recent work has revealed how changes in metabolism are closely linked to many aspects of cell behavior and the development of cancer and other diseases. This book represents both a new look at metabolism and a refresher course. It surveys the major metabolic pathways, places these in biological context, and highlights the key control points that control cell behavior and can become dysregulated in disease"--
Author: Lodovico Balducci Publisher: Springer Science & Business Media ISBN: 0387495061 Category : Medical Languages : en Pages : 148
Book Description
Anemia in the elderly has been properly defined as the silent epidemic, representing 3 million people in the United States aged 65 years and older. Incidence and prevalence of this condition increase with age. It differs in its etiology, pathogenesis and treatment from anemia in children and younger adults. Anemia is associated with reduced survival, increased risk of functional dependence and hospitalization, increased risk of congestive heart failure and stage renal disease and cognitive disorders. Approximately 70% of anemia in older individuals is reversible.
Author: Lawrence H. Lash Publisher: Elsevier ISBN: 1483218619 Category : Science Languages : en Pages : 527
Book Description
Methods in Toxicology, Volume 2: Mitochondrial Dysfunction provides a source of methods, techniques, and experimental approaches for studying the role of abnormal mitochondrial function in cell injury. The book discusses the methods for the preparation and basic functional assessment of mitochondria from liver, kidney, muscle, and brain; the methods for assessing mitochondrial dysfunction in vivo and in intact organs; and the structural aspects of mitochondrial dysfunction are addressed. The text also describes chemical detoxification and metabolism as well as specific metabolic reactions that are especially important targets or indicators of damage. The methods for measurement of alterations in fatty acid and phospholipid metabolism and for the analysis and manipulation of oxidative injury and antioxidant systems are also considered. The book further tackles additional methods on mitochondrial energetics and transport processes; approaches for assessing impaired function of mitochondria; and genetic and developmental aspects of mitochondrial disease and toxicology. The text also looks into mitochondrial DNA synthesis, covalent binding to mitochondrial DNA, DNA repair, and mitochondrial dysfunction in the context of developing individuals and cellular differentiation. Microbiologists, toxicologists, biochemists, and molecular pharmacologists will find the book invaluable.
Author: Tomas A. Salerno Publisher: John Wiley & Sons ISBN: 1405178434 Category : Medical Languages : en Pages : 344
Book Description
Myocardial protection is regarded as one of the most important, yet also most controversial aspects of cardiac surgery. There has been considerable improvement in myocardial protection strategies over recent years, utilising a variety of new approaches to treat cardiac diseases, and this text is intended to embrace the state of the art in this field. The book summarises the state of knowledge on all aspects of myocardial protection, including the latest in the treatment of cardiac diseases, robotics, pediatric surgery and the treatment of cardiac failure. Robotic surgery, valvular surgery, pediatric surgery and coronary surgery are all covered by renowned experts, producing a comprehensive, forward-looking view of the field of myocardial protection. This book should function to update physicians and surgeons interested in the field of cardiac surgery on the current state of knowledge on myocardial protection.
Author: Anne Le Publisher: Springer ISBN: 331977736X Category : Medical Languages : en Pages : 186
Book Description
Genetic alterations in cancer, in addition to being the fundamental drivers of tumorigenesis, can give rise to a variety of metabolic adaptations that allow cancer cells to survive and proliferate in diverse tumor microenvironments. This metabolic flexibility is different from normal cellular metabolic processes and leads to heterogeneity in cancer metabolism within the same cancer type or even within the same tumor. In this book, we delve into the complexity and diversity of cancer metabolism, and highlight how understanding the heterogeneity of cancer metabolism is fundamental to the development of effective metabolism-based therapeutic strategies. Deciphering how cancer cells utilize various nutrient resources will enable clinicians and researchers to pair specific chemotherapeutic agents with patients who are most likely to respond with positive outcomes, allowing for more cost-effective and personalized cancer therapeutic strategies.
Author: Sabzali Javadov Publisher: ISBN: 9783039363841 Category : Languages : en Pages : 434
Book Description
Mitochondria are subcellular organelles evolved by the endosymbiosis of bacteria with eukaryotic cells. They are the main source of ATP in the cell and engaged in other aspects of cell metabolism and cell function, including the regulation of ion homeostasis, cell growth, redox status, and cell signaling. Due to their central role in cell life and death, mitochondria are also involved in the pathogenesis and progression of human diseases/conditions, including neurodegenerative and cardiovascular disorders, cancer, diabetes, inflammation, and aging. However, despite the increasing number of studies, precise mechanisms whereby mitochondria are involved in the regulation of basic physiological functions, as well as their role in the cell under pathophysiological conditions, remain unknown. A lack of in-depth knowledge of the regulatory mechanisms of mitochondrial metabolism and function, as well as interplay between the factors that transform the organelle from its role in pro-survival to pro-death, have hindered the development of new mitochondria-targeted pharmacological and conditional approaches for the treatment of human diseases. This book highlights the latest achievements in elucidating the role of mitochondria under physiological conditions, in various cell/animal models of human diseases, and in patients.
Author: Shalise Anna Burch
Author: Shalise Anna Burch
Author: Joana Vieira Silva
Author: Navdeep Singh Chandel
Author: Bruce Alberts
Author: Lodovico Balducci
Author: Lawrence H. Lash
Author: Alexander Mauro
Author: Tomas A. Salerno
Author: Anne Le
Author: Sabzali Javadov