Author: Manlio Tassieri Publisher: CRC Press ISBN: 1315341220 Category : Science Languages : en Pages : 356
Book Description
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Author: Manlio Tassieri Publisher: CRC Press ISBN: 9814669199 Category : Science Languages : en Pages : 329
Book Description
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Author: David Riegler Publisher: ISBN: Category : Languages : en Pages : 174
Book Description
This thesis presents a method to measure the linear viscoelastic response of fluids by tracking and analyzing the thermal, Brownian motion of suspended tracer particles, known as passive microrheology. The particle is confined in a harmonic optical trap and its one dimensional trajectory is obtained by a home-built split beam detection system, which works similar but responds faster than position detection with commercial quadrant photodiodes. The theory which is necessary to convert the particle trajectory into the complex shear modulus is derived in detail, pointing out that the commonly used Mason-Weitz method needs to be modified in order to obtain correct results at high frequencies due to hydrodynamic effects of the fluid. It follows a detailed explanation of the data analysis procedure which is verified for water up to angular frequencies of 107 rad/s in very good agreement with the theory. Finally, there is an outlook how to apply the method to actual complex fluids.
Author: Kenny Breuer Publisher: Springer Science & Business Media ISBN: 3540264493 Category : Technology & Engineering Languages : en Pages : 268
Book Description
Microscale Diagnostic Techniques highlights the most innovative and powerful developments in microscale diagnostics. It provides a resource for scientists and researchers interested in learning about the techniques themselves, including their capabilities and limitations. The fields of Micro- and Nanotechnology have emerged over the past decade as a major focus of modern scientific and engineering research and technology. Driven by advances in microfabrication, the investigation, manipulation and engineering of systems characterized by micrometer and, more recently, nanometer scales have become commonplace throughout all technical disciplines. With these developments, an entirely new collection of experimental techniques has been developed to explore and characterize such systems.
Author: Philip H. Jones Publisher: Cambridge University Press ISBN: 1107051169 Category : Science Languages : en Pages : 565
Book Description
A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Author: Eric M. Furst Publisher: Oxford University Press ISBN: 0199655200 Category : Science Languages : en Pages : 473
Book Description
Rheology is the study of the flow of matter. It is an important and active field of research that spans numerous disciplines and technological applications. The aim of this work is to provide an introduction to the theory and practice of microrheology, a relatively new area of rheology.
Author: Miles J. Padgett Publisher: CRC Press ISBN: 1420074148 Category : Science Languages : en Pages : 510
Book Description
The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on
Author: Manlio Tassieri
Author: Manlio Tassieri
Author: Biljana Stojković
Author: David Riegler
Author: Randhir Kumar
Author: Kenny Breuer
Author: Philip H. Jones
Author: Eric M. Furst
Author: Nadjezjda Nijenhuis
Author: Miles J. Padgett