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Author: Publisher: ISBN: Category : Forests and forestry Languages : en Pages : 348
Book Description
Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease, insect, and pollution); and 5) biotechnological applications (wood properties, flowering, phytoremediation, breeding, commercialization, economics, and bioethics).
Author: Publisher: ISBN: Category : Forests and forestry Languages : en Pages : 348
Book Description
Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease, insect, and pollution); and 5) biotechnological applications (wood properties, flowering, phytoremediation, breeding, commercialization, economics, and bioethics).
Author: Y. P. S. Bajaj Publisher: Springer Science & Business Media ISBN: 3662093685 Category : Technology & Engineering Languages : en Pages : 339
Book Description
Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.
Author: Professor Dr. Y. P. S. Bajaj Publisher: Springer Science & Business Media ISBN: 3662093669 Category : Technology & Engineering Languages : en Pages : 432
Book Description
In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.
Author: Y. P. S. Bajaj Publisher: Springer Science & Business Media ISBN: 3642780377 Category : Technology & Engineering Languages : en Pages : 406
Book Description
In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.
Author: Y.P.S. Bajaj Publisher: Springer Science & Business Media ISBN: 3642596096 Category : Technology & Engineering Languages : en Pages : 341
Book Description
Annotation. "This volume on Transgenic Trees, comprising 22 chapters, deals with the genetic transformation of fruit and forest trees." "It is of special interest to advanced students, teachers and research workers in the field of forestry, horticulture, molecular biology, plant tissue culture, botany, and plant biotechnology in general."--BOOK JACKET. Title Summary field provided by Blackwell North America, Inc. All Rights Reserved.
Author: Byung-guk Kang Publisher: ISBN: Category : Languages : en Pages : 139
Book Description
In Chapter one, we described development of a highly efficient micropropagation protocol from greenhouse-grown shoot tips of 'Nisqually-1'. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0.3% Gelrite and 5--10 g·L−1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. In chapter II, we transferred the plant-originated Arabidopsis Atwbc19 gene encoding an ATP binding cassette transporter which confers resistance to aminoglycoside antibiotics. Transgenic plants were confirmed by polymerase chain reaction (PCR) with Atwbc19- specific primer pair. The expression was confirmed by the reverse transcription PCR. Transgenic plants were tested for aminoglycoside antibiotic resistance. The level of resistance conferred by CaMV35S-Atwbc19 is similar to that conferred by nptII gene. Therefore, plant-ubiquitous Atwbc19 gene can serve an alternative gene as a plant transformation selective marker gene to current bacterial antibiotic-resistance marker genes and alleviate the potential risk for horizontal transfer of bacterial resistance genes in transgenic plants. In Chapter III, we transformed a Populus clone with the enhancer trapping vector, pD991. All 250 transgenic lines were screened and 71 of them (28%) showed positive staining. They showed various patterns of the reporter gene expression, including expression in one tissue and simultaneously in two more tissues. These results confirmed the previously reports that enhancer trap lines can be produced in Populus, and these enhancer trap lines can be used for future gene cloning and studying gene expression in Populus. In Chapter IV, we transformed with the heavy metal binding protein agNt84 gene. Seven putative transgenic lines were confirmed by PCR with the agNt84 specific primers and two lines shoot tips of transgenic- and non-transgenic plants grown on cadmium (Cd) -containing rooting media to evaluate of Cd resistance. 33% of shoot tips from one line and 44% of those from another transgenic line survived on medium containing 250 mM Cd, respectively, but only 22% of the non-transgenic shoot tips survived on rooting medium with 150 mM Cd at week 8. Also, the Cd analysis by ICP-OES indicated that the transgenic plants which were grown on 100 mM Cd medium accumulated about 45% more Cd in the tissue than non-transgenic plants.
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Author: Ned B. Klopfenstein
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Author: Y. P. S. Bajaj
Author: Professor Dr. Y. P. S. Bajaj
Author: Y. P. S. Bajaj
Author: Y.P.S. Bajaj
Author: Byung-guk Kang
Author: Julie Ann Russell
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