Microprobe Analysis in Biology and Medicine PDF Download

Author: Patrick Echlin
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 400

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Book Description

Author: Karl Zierold
Publisher: Springer Science & Business Media
ISBN: 364274477X
Category : Science
Languages : en
Pages : 331

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Book Description
The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.

Author: Peter Ingram
Publisher:
ISBN:
Category : Diagnosis, Electron microscopic
Languages : en
Pages : 388

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Book Description
A work reviewing the applications of new microprobe analysis techniques in medicine. Authoritative chapters illustrate a variety of techniques and demonstrate appropriate applications to specific diseases. Key coverage includes pulmonary pathology and asbestoses.

Author: Yvan Llabador
Publisher: World Scientific
ISBN: 9789810223625
Category : Science
Languages : en
Pages : 292

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Book Description
"... this book will be particularly useful to the many biologists encountering problems of elemental analysis & analytical imaging who wish to have a clear yet comprehensive introduction to the principles, the conditions of application & the potential of nuclear microprobes compared with those of alternative methods." Journal of Trace & Microprobe Techniques, 1999

Author: Peter Ingram
Publisher: Academic Press
ISBN: 0080524567
Category : Science
Languages : en
Pages : 573

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Book Description
Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues.This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. Up-to-date review of electron microprobe analysis Detailed descriptions of sample preparation techniques Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy Over 100 illustrations with numerous specific applications Contributions by world-renowned experts in the field Brief summary of highlights precedes each chapter

Author: Battelle Conference Center
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 456

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Book Description
Microprobe Analysis of Biological Systems ...

Author: Claude Lechene
Publisher: Elsevier
ISBN: 0323143342
Category : Nature
Languages : en
Pages : 695

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Book Description
Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.

Author: Armand Verbueken
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Roger Theisen
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 186

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Book Description
The Electron "licroprobe X-!{ay Analyscr conceivcd b ' R C.\S'L\I: \G and A. Cl'!: '\ lEI( in 1949 has been developcd as an extremelv po\\'crful tool in spcctrochcmical analysis for a wide range of applications, ranging from qualitative elcmcntary distribution studies, to highly localiscd quantitatin analysis on a one micron scale. \\'ith the increasing number oi' versatile instruments, commcrcially available, the domain of applications - in metallurgy, solid state physics, mineralogy and geology, biology and medicine, arts and archeology - is rapidly expanding, particularly because reliable quantitative analyses can be achieved. It is well established that in multicomponent specimens, the relative x-ray intensity generated by the electron bombardment - i.e. the intensity ratio of the characteristic x-ray radiation emitted under identical experimental conditions by the specimen and a calibration standard - is not directly correlated to the elementary mass concentration. The use of a wide scale of carefully prepared homogeneous calibration standards is generally very tedious and restricted to binar)' systems. For more complex specimens, the conversion of recorded x-ra)' intensity ratios to elementary mass concentration requires, besides carefule selection of experimental conditions, an adequate correction calculation to take account oi' the various physical phenomenas occurring in the tarp;et - electron retardation, electron backseattering, x-ray excitation efficieney, fluorescence enhaneement by eharaeteristic and continuous radiation and x-ray mass absorption.

Author: M.L. Wolbarsht
Publisher: Springer Science & Business Media
ISBN: 1461307457
Category : Science
Languages : en
Pages : 307

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Book Description
The diversity of the chapters presented in this volume illustrates not only the many applications of lasers, but also the fact that, in many cases, these are not new uses of lasers, but rather improvements of laser techniques already widely accepted in both research and clinical situations. Biological reactions to some special aspects of laser exposure continue to show new effects, which have implications for the ever-present topic of laser safety. Such biological reactions are included in fields of research which depend on properties of electromagnetic radiation exposure only possible with lasers, for example, the short pulses necessary for the temperature-jump experiments reviewed by Reiss: Speciality lasers, such as the transverse excitation atmospheric (TEA) or excimer lasers, add new wavelengths and pulse domains to those already available for biological application. A description of these new types of lasers by Osgood is included to indicate new possibilities for future use and to avoid limiting our coverage to well-developed present-day applications. Hillenkamp and Kaufmann describe a microprobe mass spectrograph for analysis of the minute amounts of material evaporated by a laser pulse. The analytical possibilities of this instrument are far-reaching, and some of the various results are described to illustrate the power of their method, as well as to show the types of problems that are suitable for it. The initial steps in photosynthesis have become the subject of intensive investigation.