Microbial Physiology and Biochemistry Laboratory PDF Download

Author: David White
Publisher: Oxford University Press on Demand
ISBN: 9780195113136
Category : Science
Languages : en
Pages : 161

View

Book Description
Microbial Physiology and Biochemistry Laboratory illustrates the major features of growth and metabolism discussed in David White's The Physiology and Biochemistry of Prokaryotes (OUP, 1995). It serves as an ideal adjunct to this text and can also be used in conjunction with other books for the laboratory component of a microbial physiology course. All of the experiments described in this manual have been taught as part of a laboratory course for junior and senior biology and microbiology majors at Indiana University. In addition to reinforcing what students learn in lecture, the experiments guide students through a wide spectrum of analytical techniques including enzyme assays, macromolecular assays, column chromatography, gel electrophoresis, and gas chromatography. Along with enzyme assays and enzyme purification, students do experiments measuring oxygen uptake, chemotaxis, fermentation, and bacterial luminescence. The organisms studied include Escherichia, Pseudomonas, Bacillus, Proteus, Rhodospirillum, Photobacterium, and Saccharomyces. The volume is enhanced by appendices which include sections on quantitative problems and their solutions, instructions on how to write a laboratory report, and independent projects that are extensions of the class experiments. The number of experiments exceeds the amount of material usually offered in one semester, giving instructors the option to choose those experiments that are most appropriate for their classes.

Author: Albert G. Moat
Publisher: John Wiley & Sons
ISBN: 0471461199
Category : Science
Languages : en
Pages : 715

View

Book Description
The Fourth Edition of Microbial Physiology retains the logical, easy-to-follow organization of the previous editions. An introduction to cell structure and synthesis of cell components is provided, followed by detailed discussions of genetics, metabolism, growth, and regulation for anyone wishing to understand the mechanisms underlying cell survival and growth. This comprehensive reference approaches the subject from a modern molecular genetic perspective, incorporating new insights gained from various genome projects.

Author: David White
Publisher: Oxford University Press, USA
ISBN:
Category : Science
Languages : en
Pages : 664

View

Book Description
Describes a range of topics of interest to microbiologists, these include the structure, physiology, and biochemistry of bacteria, as well as cell-cell signaling, microbial development, and biofilm formation. The notes at the end of each chapter provide information on the topics discussed in the chapter.

Author: Byung Hong Kim
Publisher: Cambridge University Press
ISBN: 113946762X
Category : Science
Languages : en
Pages : 934

View

Book Description
Recent determination of genome sequences for a wide range of bacteria has made in-depth knowledge of prokaryotic metabolic function essential in order to give biochemical, physiological, and ecological meaning to the genomic information. Clearly describing the important metabolic processes that occur in prokaryotes under different conditions and in different environments, this advanced text provides an overview of the key cellular processes that determine bacterial roles in the environment, biotechnology, and human health. Prokaryotic structure is described as well as the means by which nutrients are transported into cells across membranes. Glucose metabolism through glycolysis and the TCA cycle are discussed, as well as other trophic variations found in prokaryotes, including the use of organic compounds, anaerobic fermentation, anaerobic respiratory processes, and photosynthesis. The regulation of metabolism through control of gene expression and control of the activity of enzymes is also covered, as well as survival mechanisms used under starvation conditions.

Author: Eldor Paul
Publisher: Academic Press
ISBN: 0123914116
Category : Technology & Engineering
Languages : en
Pages : 603

View

Book Description
The fourth edition of Soil Microbiology, Ecology and Biochemistry updates this widely used reference as the study and understanding of soil biota, their function, and the dynamics of soil organic matter has been revolutionized by molecular and instrumental techniques, and information technology. Knowledge of soil microbiology, ecology and biochemistry is central to our understanding of organisms and their processes and interactions with their environment. In a time of great global change and increased emphasis on biodiversity and food security, soil microbiology and ecology has become an increasingly important topic. Revised by a group of world-renowned authors in many institutions and disciplines, this work relates the breakthroughs in knowledge in this important field to its history as well as future applications. The new edition provides readable, practical, impactful information for its many applied and fundamental disciplines. Professionals turn to this text as a reference for fundamental knowledge in their field or to inform management practices. - New section on "Methods in Studying Soil Organic Matter Formation and Nutrient Dynamics" to balance the two successful chapters on microbial and physiological methodology - Includes expanded information on soil interactions with organisms involved in human and plant disease - Improved readability and integration for an ever-widening audience in his field - Integrated concepts related to soil biota, diversity, and function allow readers in multiple disciplines to understand the complex soil biota and their function

Author: P. Malcolm Rhodes
Publisher: IRL Press
ISBN:
Category : Culture media (Biology)
Languages : en
Pages : 304

View

Book Description
The rapid growth in biotechnology in recent years has led to an upsurge in interest in microbial technology amongst many biochemists, molecular biologists, geneticists, virologists, endocrinologists, and clinicians. Their objectives may be very diverse, ranging from the isolation of a stableenzyme from a hyperthermophile to the expression of a human protein by a recombinant yeasts or bacterium. Advance in microbial physiology have made possible a rational approach to optimization of product yield based on analysis of cultures, growth kinetics, and biochemical pathways. The applicationof statistical optimization methods, widely used in other fields, also has much to offer microbiology and biotechnology. The choice of material for this book has been influenced by both the need for practical information to enable to the isolation, handling, and culture of organisms and thenecessity to generate and analyse data enabling the development of a process. It therefore contains chapters covering the 'husbandry' of microbiology, the generation of data by chemical and physical analysis, and the interpretation of such data. Data interpretation is considered from two points ofview. Kinetic analyses of growth and product formation have frequently illuminated the development of fermentation processes. More recently, the analysis of the flux of metabolites through intermediate biochemical pathways has shown up important factors in metabolic engineering through theapplication of molecular biology techniques in microbial physiology. Applied Microbial Culture: A Practical Approach is a useful resource and guide to the successful culture of microorganisms in pure form, optimizing the culture conditions, and the scaling-up process to enable more detailedstudy.

Author: C. H. Werkman
Publisher: Elsevier
ISBN: 1483274853
Category : Nature
Languages : en
Pages : 724

View

Book Description
Bacterial Physiology focuses on the physiology and chemistry of microorganisms and the value of bacterial physiology in the other fields of biology. The selection first underscores the chemistry and structure of bacterial cells, including the chemical composition of cells, direct and indirect methods of cytology, vegetative multiplication, spores of bacteria, and cell structure. The text then elaborates on inheritance, variation, and adaptation and growth of bacteria. The publication reviews the physical and chemical factors affecting growth and death. Topics include hydrogen ion concentration and osmotic pressure; surface and other forces determining the distribution of bacteria in their environment; dynamics of disinfection and bacteriostasis; bacterial resistance; and types of antibacterial agents. The text also ponders on the anaerobic dissimilation of carbohydrates, bacterial oxidations, and autotrophic assimilation of carbon dioxide. The selection is a dependable reference for readers interested in bacterial physiology.

Author: Robert A. Pollack
Publisher: John Wiley & Sons
ISBN: 1119462665
Category : Science
Languages : en
Pages : 322

View

Book Description
The Laboratory Exercises in Microbiology, 5e by Pollack, et al. presents exercises and experiments covered in a 1 or 2-semester undergraduate microbiology laboratory course for allied health students. The labs are introduced in a clear and concise manner, while maintaining a student-friendly tone. The manual contains a variety of interactive activities and experiments that teach students the basic concepts of microbiology. The 5th edition contains new and updated labs that cover a wide array of topics, including identification of microbes, microbial biochemistry, medical microbiology, food microbiology, and environmental microbiology.

Author: S. Ram Reddy
Publisher: Scientific Publishers
ISBN: 9387913198
Category : Technology & Engineering
Languages : en
Pages : 350

View

Book Description
This book, the first of its kind by Indian authors, tries to develop a comprehensive understanding of microbial metabolism. It deals with all basic and unique physiological aspects of microorganisms in an ordered sequence with profuse illustrations. Discussion of all chapters is based on the concepts of bioenergetics which form the life-line of metabolic functions. It provides the foundation and general frame work for further understanding of the subject. This book not only serves as a text for undergraduate, post-graduate students, but also as a reference book to teachers, researchers and all others interested in the metabolism of the microorganisms in particular and living organisms in general.

Author: G. N. Cohen
Publisher: Springer
ISBN: 9401789088
Category : Science
Languages : en
Pages : 618

View

Book Description
Microbial physiology, biochemistry and genetics allowed the formulation of concepts that turned out to be important in the study of higher organisms. In the first section, the principles of bacterial growth are given, as well as the description of the different layers that enclose the bacterial cytoplasm, and their role in obtaining nutrients from the outside media through different permeability mechanism described in detail. A chapter is devoted to allostery and is indispensable for the comprehension of many regulatory mechanisms described throughout the book. Another section analyses the mechanisms by which cells obtain the energy necessary for their growth, glycolysis, the pentose phosphate pathway, the tricarboxylic and the anaplerotic cycles. Two chapters are devoted to classes of microorganisms rarely dealt with in textbooks, namely the Archaea, mainly the methanogenic bacteria, and the methylotrophs. Eight chapters describe the principles of the regulations at the transcriptional level, with the necessary knowledge of the machineries of transcription and translation. The next fifteen chapters deal with the biosynthesis of the cell building blocks, amino acids, purine and pyrimidine nucleotides and deoxynucleotides, water-soluble vitamins and coenzymes, isoprene and tetrapyrrole derivatives and vitamin B12. The two last chapters are devoted to the study of protein-DNA interactions and to the evolution of biosynthetic pathways. The considerable advances made in the last thirty years in the field by the introduction of gene cloning and sequencing and by the exponential development of physical methods such as X-ray crystallography or nuclear magnetic resonance have helped presenting metabolism under a multidisciplinary attractive angle.