Microbial and Plant Protoplasts PDF Download

Author: John F. Peberdy
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 384

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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Author: Tessa Bengochea
Publisher: Springer Science & Business Media
ISBN: 9400940955
Category : Science
Languages : en
Pages : 97

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Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.

Author: J. Peberdy
Publisher: CRC Press
ISBN: 9780824771126
Category : Science
Languages : en
Pages : 378

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Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

Author: Ingo Potrykus
Publisher: Birkhäuser
ISBN:
Category : Juvenile Nonfiction
Languages : en
Pages : 280

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Recent developments in the culture and regeneration of plant protoplasts; Protoplast culture and plant regeneration of cereals and other recalcitrant crops; Protoplasts and the isolation of plant mutants; Protoplasts and variation from culture; Systems for plant protoplast transformation; Recent developments in plant protoplast fusion and selection technology; Somatic hybridization by plant protoplast fusion; The segregation of organelles and cytoplasmic traits in higher plant somatic fusion hybrids; The first mitotic cycle of mesophyll protoplasts; Protoplasts for studies of the plasma membrane and associated cell organelles; The use of protoplasts in plant virus research; Applications of protoplast technology to agriculture; Organelle transfer, sorting out, recombination; Plant protoplasts as tools for physiological studies; Genetic transformation; Protoplasts as tools in pathology, virology and plant-microbe interactions; Current questions of gene transfer via protoplast fusion in microorganisms (Opening adress); Advances in protoplast fusion and transformation in Streptomyces; Chromosome interactions and expression in fused Bacillus protoplasts; Yeast strain improvement by protoplast fusion and transformation; Protoplasts of filamentous fungi in genetics and metabolite production; Interspecific somatic hybridisation in Aspergillus; Synthesis and assembly of wall polymers on regenerating yeast protoplasts; The theory and practical applications of liposome-protoplast interactions; Transport of nutrients in yeast protoplast; Protoplasts and spheroplasts of gram-negative bacteria - with special emphasis on proteus mirabilis; Characteristic properties and biological significance of stable protoplast type L-forms; Advances in microbial protoplasts (Bacillus licheniformis lactamase and the protoplast surface); Fungal protoplasts as genetic tools; Non-complementing diploids: prokaryotic microorganism protoplast fusion and chromosome inactivation.

Author: Fowke
Publisher: CRC Press
ISBN: 1351084224
Category : Science
Languages : en
Pages : 254

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The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.

Author: Professor Dr. Y. P. S. Bajaj
Publisher: Springer Science & Business Media
ISBN: 3662093669
Category : Technology & Engineering
Languages : en
Pages : 432

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In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309034345
Category : Science
Languages : en
Pages : 97

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"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."

Author: Julio Rodríguez Villanueva
Publisher:
ISBN:
Category : Nature
Languages : en
Pages : 416

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Yeast cell wall lytic ensymes; Structure and morphogenesis; Physiology and biochemistry of yeast protoplasts; Protoplasts of moulds; Protoplasts of plants.

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254

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Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.