Microbes in Restricted Indoor Environments - with Focus on the International Space Station and Spacecraft Associated Clean Rooms PDF Download

Author: Maximilian Mora
Publisher:
ISBN:
Category :
Languages : en
Pages : 266

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Author: Rakesh Mogul
Publisher: Frontiers Media SA
ISBN: 2889763595
Category : Science
Languages : en
Pages : 225

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Author: Brian Flannigan
Publisher: CRC Press
ISBN: 0203302931
Category : Science
Languages : en
Pages : 555

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Despite the large amount of money spent on research into pollution of the indoor environment, the problem remains complex with major gaps in our knowledge of the identities and sources of pollutants and of the effects of prolonged exposure to indoor pollutants on health. Microorganisms in Home and Indoor Work Environments considers one such group o

Author: Brian Flannigan
Publisher: CRC Press
ISBN: 1420093355
Category : Nature
Languages : en
Pages : 529

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In 2007, scientists estimated the direct cost of diseases associated with mould and dampness on the US population to be in the range of 4 billion dollars, and the indirect costs of lost work and school days are gauged even higher. The US Centers for Disease Control recently concluded that elimination of moisture and mouldy materials in the home def

Author: National Aeronautics and Space Adm Nasa
Publisher: Independently Published
ISBN: 9781723831126
Category : Science
Languages : en
Pages : 44

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Spacecraft such as the International Space Station (ISS) and the space shuttles are enclosed environments where crewmembers may spend long periods of time. Currently, crewmembers spend approximately a period of 6 months in the ISS. It is known that these prolonged stays in space may result in weakening of the immune system. Therefore, exposure to opportunistic pathogens or high concentrations of environmental microorganisms may compromise the health of the crew. The detection of biocontaminants in spacecraft environments utilizes culture-based methodology, omitting greater than 90% of all microorganisms including pathogens such as Legionella and Cryptosporidium. Culturable bacteria and fungi have been the only allergens studied; the more potent allergens, such as those from dust mites, have never been tested for in spacecraft environments. In addition, no attempts have been made to monitor microbial toxins in spacecrafts. The present study utilized quantitative polymerase chain reaction (QPCR) as a novel approach for monitoring microorganisms in the spacecraft environment. QPCR is a molecular biology technique that does not rely on the physiological state of the organisms for identification, thereby enabling detection of both culturable and non-culturable organisms. In this project, specific molecular primers and probes were utilized for the detection and quantitation of two fungi of concern in indoor environments, Aspergillus fumigatus and Stachybotrys chartarum. These organisms were selected because of the availability of PCR primers and probes, and to establish the sample processing and analysis methodology that may be employed with additional organisms. Purification methods and QPCR assays were optimized for the detection of these organisms in air, surface, and water; and sample processing and analysis protocols were developed. Preliminary validation of these protocols was conducted in the laboratory with air, surface, and water samples seeded with known concent

Author: Helga Stan-Lotter
Publisher: Springer Science & Business Media
ISBN: 3211996907
Category : Science
Languages : en
Pages : 287

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Once considered exceptional rarities, extremophiles have become attractive objects for basic and applied research ranging from nanotechnology to biodiversity to the origins of life and even to the search for extraterrestrial life. Several novel aspects of extremophiles are covered in this book; the focus is firstly on unusual and less explored ecosystems such as marine hypersaline deeps, extreme cold, desert sands, and man-made clean rooms for spacecraft assembly. Secondly, the increasingly complex field of applications from extremophile research is treated and examples such as novel psychrophilic enzymes, compounds from halophiles, and detection strategies for potential extraterrestrial life forms are presented.

Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309449839
Category : Science
Languages : en
Pages : 318

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People's desire to understand the environments in which they live is a natural one. People spend most of their time in spaces and structures designed, built, and managed by humans, and it is estimated that people in developed countries now spend 90 percent of their lives indoors. As people move from homes to workplaces, traveling in cars and on transit systems, microorganisms are continually with and around them. The human-associated microbes that are shed, along with the human behaviors that affect their transport and removal, make significant contributions to the diversity of the indoor microbiome. The characteristics of "healthy" indoor environments cannot yet be defined, nor do microbial, clinical, and building researchers yet understand how to modify features of indoor environmentsâ€"such as building ventilation systems and the chemistry of building materialsâ€"in ways that would have predictable impacts on microbial communities to promote health and prevent disease. The factors that affect the environments within buildings, the ways in which building characteristics influence the composition and function of indoor microbial communities, and the ways in which these microbial communities relate to human health and well-being are extraordinarily complex and can be explored only as a dynamic, interconnected ecosystem by engaging the fields of microbial biology and ecology, chemistry, building science, and human physiology. This report reviews what is known about the intersection of these disciplines, and how new tools may facilitate advances in understanding the ecosystem of built environments, indoor microbiomes, and effects on human health and well-being. It offers a research agenda to generate the information needed so that stakeholders with an interest in understanding the impacts of built environments will be able to make more informed decisions.

Author: David M. Carlberg
Publisher: CRC Press
ISBN: 9780935184730
Category : Medical
Languages : en
Pages : 168

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Written for the professional who has an immediate need for the information but has little or no training in the subject, Cleanroom Microbiology for the Non-Microbiologist introduces principles of microbiology. It explains the consequences of microbiological contamination, what contamination is all about, how microorganisms grow, and how they can be controlled. The book introduces the vocabulary of microbiology and the types, sources, control, and elimination of organisms encountered in the manufacture of sterile products. Beginning with a discussion of the various types of organisms, the text then covers applications for bacterial detection, avoidance of contamination, cleanroom design considerations, and validation of disinfection methods. This book is especially relevant for supervisors and managers who need a sound understanding of contamination control and is particularly effective for use in training. It also is an excellent resource for validation engineers, quality assurance and non-microbiologist quality-control staff, maintenance personnel, and process development staff.

Author: Marsha R. Torr
Publisher:
ISBN:
Category : Contamination (Technology)
Languages : en
Pages : 156

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Author: Chin S. Yang
Publisher: John Wiley & Sons
ISBN: 0470112425
Category : Technology & Engineering
Languages : en
Pages : 291

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Book Description
Investigation techniques and analytical methodologies for addressing microbial contamination indoors Microbial contamination indoors is a significant environmental and occupational health and safety problem. This book provides fundamental background information on fungal and bacterial growth indoors as well as in-depth, practical approaches to analyzing and remedying problems. The information helps investigators, laboratory managers, and environmental health professionals properly use state-of-the-science methods and correctly interpret the results. With chapters by expert microbiologists, mycologists, environmental professionals, and industrial hygienists, Sampling and Analysis of Indoor Microorganisms is a multidisciplinary, comprehensive reference on advanced approaches, covering: Microbiological problems in a water-damaged environment Indoor construction techniques and materials that impact environmental microbiology Microbial ecology indoors, airborne bacteria, genetic-based analytical methods, and statistical tools for microorganism analysis Microbiological sampling approaches Mold removal principles and methods, including specialized microbial remediation techniques for HVAC systems, legionellas and biofilms, and sewage contamination A forensic approach toward the assessment of fungal growth in the indoor environment A must-have guide for practicing professionals, including environmental health and safety personnel, public health officials, and building and construction engineers and architects, this is also a valuable reference for attorneys, home inspectors, water restoration personnel, mold remediation contractors, insurance adjusters, and others.