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Author: Tom Moss Publisher: Springer Science & Business Media ISBN: 0896036251 Category : Medical Languages : en Pages : 632
Book Description
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA protein interactions.
Author: Tom Moss Publisher: Springer Science & Business Media ISBN: 0896036251 Category : Medical Languages : en Pages : 632
Book Description
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA protein interactions.
Author: Jost Publisher: Birkhäuser ISBN: 3034875614 Category : Science Languages : en Pages : 319
Book Description
A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.
Author: Andrew D. Bates Publisher: Oxford University Press, USA ISBN: 9780198567097 Category : Science Languages : en Pages : 224
Book Description
"A key aspect of DNA is its ability to form a variety of structures, this book explains the origins and importance of such structures"--Provided by publisher.
Author: W. Guschlbauer Publisher: Springer Science & Business Media ISBN: 1468453831 Category : Science Languages : en Pages : 289
Book Description
This volume contains the texts of the nineteen lectures presented at the NATO-ASI - FEBS Course on "DNA - ligand interactions: from drugs to proteins." The Advanced Study Institute (ASIl was held from August 30th to September 11th. 1986 in the Abbey of Fontevraud (France). The ASI was attended by 112 participants from a wide scientific horizon and from twentyone different countries. It was in some way a follow-up of the ASI held in Maratea. Italy in May 1981 and which was published in the NATO ASI Life Science series as volume 45. While much has been learned about the way the cellular machinery maintains and transmits the genetic heritage. as well as how these processes are regulated. little is Known about how the interactions between the various partners involved are taKing place. The interactions of drugs and proteins with nucleic acids are of evident importance in the understanding of these problems. The spectacular advances in recombinant DNA technology and the increased sophistication of biophysical techniques. in particular >:-ray diffraction and nuclear magnetic resonance. have created a scientific environment which is highly promising for the future of research in molecular biology. These advances permH the serious hope that biology on the molecular level may become a r-eality. Some of the contributions at the ASI presented the most recent advances in this e>:citing field.
Author: Peter Membrey Publisher: Apress ISBN: 1430230525 Category : Computers Languages : en Pages : 317
Book Description
MongoDB, a cross-platform NoSQL database, is the fastest-growing new database in the world. MongoDB provides a rich document-oriented structure with dynamic queries that you’ll recognize from RDBMS offerings such as MySQL. In other words, this is a book about a NoSQL database that does not require the SQL crowd to re-learn how the database world works! MongoDB has reached 1.0 and boasts 50,000+ users. The community is strong and vibrant and MongoDB is improving at a fast rate. With scalable and fast databases becoming critical for today’s applications, this book shows you how to install, administer and program MongoDB without pretending SQL never existed.
Author: N Manikanda Boopathi Publisher: Springer Science & Business Media ISBN: 8132209583 Category : Science Languages : en Pages : 304
Book Description
Genetic mapping and marker assisted selection (MAS) is considered as one of the major tools in genetic improvement of crop plants in this genomics era. This book describes basics in linkage mapping, step-by-step procedure to perform MAS, achievements made so far in different crops, and limitations and prospects of MAS in plant breeding. It summarizes all this in a simple but comprehensive mode using suitable examples so as to explain the concept and its historical developments. To summarize, this book describes technologies for identification of genes of interest through genetic mapping, recaps the major applications of MAS to plant breeding; lists examples in which MAS is being applied to various breeding programs, and emphasizes the various difficulties that limit the application of MAS in plant breeding, providing possible solutions to overcome these difficulties, and finally tries to illustrate the future prospects. This book would be a valuable guide to the under-graduates and post-graduates of agricultural universities and institutes that are interested and/or involved in genetic improvement of crop plants using modern tools. Bibliography listed in this book constitutes two parts: literature cited and further reading. Literature cited contains references cited in the text and further information on the given concept/technique can be obtained from these references. Further reading provides a list of suggested readings for in-depth coverage of the topics.
Author: Visith Thongboonkerd Publisher: Karger Medical and Scientific Publishers ISBN: 3805576366 Category : Medical Languages : en Pages : 344
Book Description
The novel discipline of proteomics has experienced a rapid growth in the recent past and has a great potential for the future. Involving several fields, it offers various advantages: The study of proteins on a genomic scale allows to examine a large number of them simultaneously. Moreover, proteomic analysis provides information about post-translational modifications and functions of gene products or proteins that cannot be obtained by genomic analysis. The knowledge thus gained is also important for a better understanding of renal physiology and pathophysiology, to identify disease biomarkers, and to develop new target therapies.This book is the first one that applies the science of proteomics to nephrology: It sets out to give an overview of the discipline, providing detailed background information and outlining scope, advantages, and limitations of proteomics.The second section reviews principles of commonly used proteomic techniques, including sample preparation, two-dimensional gel electrophoresis, various types of mass spectrometry, expression proteomics, quantitative proteomics, practical bioinformatics, and clinical proteomics. In the last section, examples and perspectives are provided of how proteomics can be applied to various fields in nephrology, including nephrotoxicity, sodium transport, diabetic nephropathy, renal Fanconi syndrome, IgA nephropathy, physiology of podocytes and mesangial cells, hypertension, urological malignancies, and dialysis. The publication ends with a discussion of biomarker discovery in glomerular diseases and drug discovery.Written by acclaimed experts in both proteomics and nephrology, this truly remarkable book is essential reading for students, residents, fellows, scientists, and nephrologists who are interested in or devoted to research, as well as for general health professionals and clinicians.
Author: Tom Moss
Author: Tom Moss
Author: Jost
Author: Andrew D. Bates
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Author: W. Guschlbauer
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Author: Peter Membrey
Author: N Manikanda Boopathi
Author: Visith Thongboonkerd
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