Author: Publisher:
ISBN:
Category :
Languages : en
Pages : 398
Book Description
Manipulation of Gene Expression in Early Mouse Embryo
Author: Genetic Manipulation of the Early Mammalian Embryo
Author: Frank CostantiniPublisher:
ISBN:
Category : Science
Languages : en
Pages : 312
Book Description
Manipulating the Mouse Embryo
Author: Richard BehringerPublisher:
ISBN: 9781936113019
Category : MEDICAL
Languages : en
Pages : 0
Book Description
Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.
Manipulating the Mouse Embryo
Author: Brigid HoganPublisher: Cold Spring Harbor Laboratory Press
ISBN:
Category : Nature
Languages : en
Pages : 352
Book Description
Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.
Safety of Genetically Engineered Foods
Author: National Research CouncilPublisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254
Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Scientific Frontiers in Developmental Toxicology and Risk Assessment
Author: National Research CouncilPublisher: National Academies Press
ISBN: 0309070864
Category : Nature
Languages : en
Pages : 348
Book Description
Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Gene Expression During Preimplantation Development in the Mouse Embryo
Author: Fanyi ZengMaternal Control of Development in Vertebrates
Author: Florence Louise MarlowPublisher: Morgan & Claypool Publishers
ISBN: 161504051X
Category : Family & Relationships
Languages : en
Pages : 221
Book Description
Eggs of all animals contain mRNAs and proteins that are supplied to or deposited in the egg as it develops during oogenesis. These maternal gene products regulate all aspects of oocyte development, and an embryo fully relies on these maternal gene products for all aspects of its early development, including fertilization, transitions between meiotic and mitotic cell cycles, and activation of its own genome. Given the diverse processes required to produce a developmentally competent egg and embryo, it is not surprising that maternal gene products are not only essential for normal embryonic development but also for fertility. This review provides an overview of fundamental aspects of oocyte and early embryonic development and the interference and genetic approaches that have provided access to maternally regulated aspects of vertebrate development. Some of the pathways and molecules highlighted in this review, in particular, Bmps, Wnts, small GTPases, cytoskeletal components, and cell cycle regulators, are well known and are essential regulators of multiple aspects of animal development, including oogenesis, early embryogenesis, organogenesis, and reproductive fitness of the adult animal. Specific examples of developmental processes under maternal control and the essential proteins will be explored in each chapter, and where known conserved aspects or divergent roles for these maternal regulators of early vertebrate development will be discussed throughout this review. Table of Contents: Introduction / Oogenesis: From Germline Stem Cells to Germline Cysts / Oocyte Polarity and the Embryonic Axes: The Balbiani Body, an Ancient Oocyte Asymmetry / Preparing Developmentally Competent Eggs / Egg Activation / Blocking Polyspermy / Cleavage/ Mitosis: Going Multicellular / Maternal-Zygotic Transition / Reprogramming: Epigenetic Modifications and Zygotic Genome Activation / Dorsal-Ventral Axis Formation before Zygotic Genome Activation in Zebrafish and Frogs / Maternal TGF-β and the Dorsal-Ventral Embryonic Axis / Maternal Control After Zygotic Genome Activation / Compensation by Stable Maternal Proteins / Maternal Contributions to Germline Establishment or Maintenance / Perspective / Acknowledgments / References
Manipulating the Mouse Embryo
Author: Brigid HoganPublisher:
ISBN: 9780879693923
Category :
Languages : en
Pages : 500
Book Description
Molecular Biology of The Cell
Author: Bruce AlbertsPublisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
Book Description