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Author: Eugene A. Permyakov Publisher: CRC Press ISBN: 1351091115 Category : Medical Languages : en Pages : 224
Book Description
Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.
Author: Eugene A. Permyakov Publisher: CRC Press ISBN: 1351091115 Category : Medical Languages : en Pages : 224
Book Description
Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.
Author: Eugene A. Permyakov Publisher: CRC Press ISBN: 1351082663 Category : Medical Languages : en Pages : 172
Book Description
Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.
Author: Joseph R. Lacowicz Publisher: Springer Science & Business Media ISBN: 0306471027 Category : Science Languages : en Pages : 320
Book Description
The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.
Author: Joseph R. Lakowicz Publisher: Springer Science & Business Media ISBN: 0387463127 Category : Science Languages : en Pages : 961
Book Description
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
Author: Gregor Jung Publisher: Springer Science & Business Media ISBN: 3642233767 Category : Science Languages : en Pages : 287
Book Description
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
Author: Alexander P. Demchenko Publisher: Springer Science & Business Media ISBN: 3642708471 Category : Science Languages : en Pages : 323
Book Description
The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.
Author: Vladimir N. Uversky Publisher: Nova Publishers ISBN: 9781600217036 Category : Science Languages : en Pages : 326
Book Description
Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
Author: Eugene A. Permyakov
Author: Eugene A. Permyakov
Author: Eugene A. Permyakov
Author: Vladimir N. Uversky
Author: Nicole Danielle Bouley Ford
Author: Joseph R. Lacowicz
Author: Joseph R. Lakowicz
Author: Gregor Jung
Author: Alexander P. Demchenko
Author: Vladimir N. Uversky
Author: I︠U︡rifĭ Andreevich Vladimirov