Lipases and Phospholipases PDF Download

Author: Georgina Sandoval
Publisher: Humana
ISBN: 9781493993611
Category : Science
Languages : en
Pages : 437

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Book Description
This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.

Author: Mark Doolittle
Publisher: Springer Science & Business Media
ISBN: 1592595812
Category : Science
Languages : en
Pages : 362

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Book Description
The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.

Author: Uwe T. Bornscheuer
Publisher: Elsevier
ISBN: 0128131683
Category : Technology & Engineering
Languages : en
Pages : 396

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Book Description
Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts. - Identifies how, and when, to use enzymes and microbes for lipid modification - Provides enzymatic, microbial and metabolic techniques for lipid modification - Covers lipases, acyltransferases, phospholipases, lipoxygenases, monooxygenases, isomerases and sophorolipids - Includes lipid modification for use in food, biofuels, oleochemicals and polymer precursors

Author: Hans Brockerhoff
Publisher: Elsevier
ISBN: 0323155162
Category : Science
Languages : en
Pages : 343

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Book Description
Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface. Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book. This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.

Author: Paul Woolley
Publisher: Cambridge University Press
ISBN: 9780521207997
Category : Science
Languages : en
Pages : 0

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Book Description
This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.

Author: Uwe Theo Bornscheuer
Publisher: John Wiley & Sons
ISBN: 3527607129
Category : Science
Languages : en
Pages : 368

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Book Description
From reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.

Author: Tsung Min Kuo
Publisher: CRC Press
ISBN: 9780203908198
Category : Science
Languages : en
Pages : 748

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Book Description
This text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding. It offers an in-depth review of the most recent principles and approaches utilized in the development and design of lipids for cosmetic, industrial and pharmaceutical, and food products. Discussing a variety of lipid-ac

Author: Günter Müller
Publisher: John Wiley & Sons
ISBN: 3527605703
Category : Science
Languages : en
Pages : 354

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Book Description
Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.

Author: John W. Pelley, PhD
Publisher: Elsevier Health Sciences
ISBN: 0323074464
Category : Medical
Languages : en
Pages : 254

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Book Description
Effectively merge basic science and clinical skills with Elsevier's Integrated Review Biochemistry, by John W. Pelley, PhD. This concise, high-yield title in the popular Integrated Review Series focuses on the core knowledge in biochemistry while linking that information to related concepts from other basic science disciplines. Case-based questions at the end of each chapter enable you to gauge your mastery of the material, and a color-coded format allows you to quickly find the specific guidance you need. Online access via www.studentconsult.com - included with your purchase - allows you to conveniently access the book's complete text and illustrations online as well as relevant content from other Student Consult titles. This concise and user-friendly reference provides crucial guidance for the early years of medical training and USMLE preparation. Spend more time reviewing and less time searching thanks to an extremely focused, "high-yield" presentation. Gauge your mastery of the material and build confidence with both case-based, andUSMLE-style questions that provide effective chapter review and quick practice for your exams. Access the full contents online at www.studentconsult.com where you'll find the complete text and illustrations, "Integration Links" to bonus content in other Student Consult titles, an interactive community center with a wealth of additional resources, and much more! Grasp and retain vital concepts more easily thanks to a color-coded format, succinct,text, key concept boxes, and dynamic illustrations that facilitate learning in a highly visual approach. Effectively review for problem-based courses with the help of text boxes that help you clearly see the clinical relevance of the material. Great for visual learners!

Author: Juan-Luis Ramos
Publisher: Springer Science & Business Media
ISBN: 0387288813
Category : Science
Languages : en
Pages : 367

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Book Description
The first three volumes of the Pseudomonas series covered the biology of pseudomonas in a wide context, including the niches they inhabit, the taxonomic relations among members of this group, the molecular biology of gene expression in different niches and under different environmental conditions, the analysis of virulence traits in plants, animals and human pathogens as well as the determinants that make some strains useful for biotechnological applications and promotion of plant growth.Pseudomonas volume 4 is intended to collect some of the most relevant new emerging issues in the field of Pseudomonas that were not assembled in the three previous volumes.This fourth volume covers the following topics:Virulence and PathogensGenomics and ProteomicsPhysiology, Metabolism and Biotechnology.nbsp;Pseudomonas volume 4 will be of use to researchers working on these bacteria, particularly those studying virulence, genomics, physiology, biotechnology, etc. Advanced students in biology, medicine and agronomy will also find this volume a valuable reference during their studies.