Light Scattering Studies of Mononucleosomal DNA PDF Download

Author: Marilyn Elizabeth Ferrari
Publisher:
ISBN:
Category :
Languages : en
Pages : 294

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Author: Kenneth S Schmitz
Publisher: Elsevier
ISBN: 0323140351
Category : Science
Languages : en
Pages : 470

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Book Description
An Introduction to Dynamic Light Scattering by Macromolecules provides an introduction to the basic concepts of dynamic light scattering (DLS), with an emphasis on the interpretation of DLS data. It presents the appropriate equations used to interpret DLS data. The material is presented in order of increasing complexity of the systems under examination, ranging from dilute solutions of noninteracting particles to concentrated multicomponent solutions of strongly interacting particles and gels. Problems are presented at the end of each chapter to emphasize these concepts. Since a major emphasis of this textbook is the interpretation of DLS data obtained by polarized light scattering studies on macromolecular solutions, the results of complementary experimental techniques are also presented in order to gain insight into the dynamics of these systems. This textbook is intended for (1) advanced undergraduate students and graduate students in the chemical, physical, and biological sciences; (2) scientists who might wish to apply DLS methods to systems of interest to them but who have no formal training in the field of DLS; and (3) those who are simply curious as to the type of information that might be obtained from DLS techniques.

Author: Robert Pecora
Publisher: SPIE-International Society for Optical Engineering
ISBN:
Category : Medical
Languages : en
Pages : 386

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Author: G.D. Fasman
Publisher: Springer Science & Business Media
ISBN: 1475725086
Category : Science
Languages : en
Pages : 739

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Book Description
''Excellent and very timely....It will undoubtedly become a standard reference for the application of circular dichroism (CD) to biomolecules.'' --- Quarterly Review of Biology, March 1997 ''[T]estament to the book's utility is the fact that during the course of my review I had to 'rescue' it from the desks of graduate students on an almost daily basis. In summary, this is a great book.'' --- American Scientist ''Well documented chapters provide a very good insight into the problems surrounding the conformation of biomacromolecules...An indispensible source of information.'' --- Nahrung, 42(2), 1998 Renowned experts present the first state-of-the-art description of circular dichroism spectroscopy (CD). Chapters present in-depth discussions of the history of the field, the theory of CD for application to globular proteins, membrane proteins, peptides, nucleic acids and their interactions, carbohydrates, and instrumentation. Discussions also feature new techniques using synchrotron radiation, vibrational Raman optical activity, and vibrational CD. More than 250 illustrations supplement the text.

Author: R. Pecora
Publisher: Springer Science & Business Media
ISBN: 9780306417900
Category : Science
Languages : en
Pages : 442

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Book Description
In the twenty years since their inception, modern dynamic light-scattering techniques have become increasingly sophisticated, and their applications have grown exceedingly diverse. Applications of the techniques to problems in physics, chemistry, biology, medicine, and fluid mechanics have prolifer ated. It is probably no longer possible for one or two authors to write a monograph to cover in depth the advances in scattering techniques and the main areas in which they have made a major impact. This volume, which we expect to be the first of aseries, presents reviews of selected specialized areas by renowned experts. It makes no attempt to be comprehensive; it emphasizes a body of related applications to polymeric, biological, and colloidal systems, and to critical phenomena. The well-known monographs on dynamic light scattering by Berne and Pecora and by Chu were published almost ten years ago. They provided comprehensive treatments of the general principles of dynamic light scat tering and gave introductions to a wide variety of applications, but natu rally they could not treat the new applications and advances in older ones that have arisen in the last decade. The new applications include studies of interacting particles in solution (Chapter 4); scaling approaches to the dynamics of polymers, including polymers in semidilute solution (Chapter 5); the use of both Fabry-Perot interferometry and photon correlation spectroscopy to study bulk polymers (Chapter 6); studies of micelIes and microemulsions (Chapter 8); studies of polymer gels (Chapter 9).

Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 892

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Author: J. Zlatanova
Publisher: Gulf Professional Publishing
ISBN: 9780444515940
Category : Medical
Languages : en
Pages : 546

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Book Description
Biological processes that replicate, preserve and use the genetic information encoded in DNA must operate in the context of chromatin, a highly organized complex of DNA and proteins. These proteins do not merely package the DNA in the tiny volume of the nucleus, but impart the structure the ability to change according to the requirements of the specific process the DNA is involved in. Moreover, chromatin structure is used by the cell to control the activity of DNA. In this volume the basics of chromatin structure and dynamics are presented by established experts in the field.

Author: Benjamin Chu
Publisher:
ISBN:
Category : Technology & Engineering
Languages : en
Pages : 744

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Author: Rita Dias
Publisher: John Wiley & Sons
ISBN: 0470286350
Category : Technology & Engineering
Languages : en
Pages : 550

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Book Description
A broad overview of the interaction of DNA with surfactants and polymers Due to the potential benefits of biotechnology, interest in the interaction between DNA and surfactants and polymers has become increasingly significant. Now, DNA Interactions with Polymers and Surfactants provides an extensive, up-to-date overview of the subject, giving readers a basis for understanding the factors leading to complexation between DNA and different cosolutes, including metal ions, polyelectrolytes, spermine, spermidine, surfactants and lipids, and proteins. Topical coverage includes: Polyelectrolytes, physico-chemical aspects and biological significance Solution behavior of nucleic acids Single DNA molecules: compaction and decompaction Interaction of DNA with surfactants and cationic polymers Interactions of histones with DNA DNA-DNA interactions The hydration of DNA-amphiphile complexes DNA-surfactant/lipid complexes at liquid interfaces DNA and DNA-surfactant complexes at solid surfaces The role of correlation forces for DNA-cosolute interactions Simulations of polyions Cross-linked DNA gels and gel particles DNA as an amphiphilic polymer Lipid-DNA interactions Covering both theoretical and practical aspects of the subject, DNA Interactions with Polymers and Surfactants is an ideal resource for chemists and biochemists working in gene and DNA delivery research in industry and academia, as well as for cell biologists, chemical engineers, molecular biologists, and development biologists in the pharmaceutical industry.

Author: Nikos Theodorakopoulos
Publisher: World Scientific
ISBN: 9811209553
Category : Science
Languages : en
Pages : 196

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Book Description
The stability of the DNA double helix is contingent on fine-tuning a number of physicochemical control parameters. Varying any one of them leads to separation of the two strands, in what constitutes a rare physical example of a thermodynamic phase transition in a one-dimensional system. The present book aims at providing a self-contained account of the statistical physics of cooperative processes in DNA, e.g. thermal and mechanical dissociation, force-induced melting, equilibria of hairpin-like secondary structures. In addition, the book presents some fundamental aspects of DNA elasticity, as observed in key experiments, old and new. The latter include some recently published scattering data on apparently soft, short DNA chains and their interpretation in terms of local structural defects (permanent bends, 'kinky DNA', after the original Crick-Klug hypothesis).The development of mathematical models used (Kratky-Porod polymer chain, Poland-Scheraga and Peyrard-Bishop-Dauxois models of DNA melting) emphasizes the use of realistic parameters and the relevance of practical numerical methods for comparing with experimental data. Accordingly, a large number of specially produced figures has been included.The presentation is at the level of an advanced undergraduate or introductory graduate course. An extra chapter provides the necessary mathematical background on elasticity of model polymer chains.