Laser-induced Transfer of Human Mesenchymal Cells Using Near Infrared Femtosecond Laser Pulses for the Precise Configuration of Cell Nichoids PDF Download

Author: Jun Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: Muhammad Waleed
Publisher: LAP Lambert Academic Publishing
ISBN: 9783659646485
Category :
Languages : en
Pages : 56

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Book Description
In this book, we demonstrate a new single cell optoporation and transfection technique using femtosecond Gaussian laser beam and optical tweezers. Tightly focused near-infrared (NIR) femtosecond laser pulse was employed to transiently perforate the cellular membrane at a single point of MCF-7 cancer cell. A distinct technique is developed by trapping the microparticle using optical tweezers to focus femtosecond laser exactly on the cell membrane to puncture it for the desired time. Subsequently, calculated amount of external gene was introduced in the cell by trapping and inserting same plasmid coated microparticle into optoporated cell using optical tweezers. Various experimental parameters such as femtosecond laser exposure power, exposure time, punctured hole size, exact focusing of femtosecond laser and cell healing time were closely analyzed to optimize the best conditions for cell viability. Trapped particle position is detected by another co aligned continuous wave laser. In the end, after insertion of plasmid coated microparticle, the targeted cells exhibited green fluorescent protein (GFP) under the fluorescent microscope hence confirming successful genetic change.

Author: Aisada Uchugonova
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Author: Michael W. Berns
Publisher: Academic Press
ISBN:
Category : Medical
Languages : en
Pages : 824

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Book Description
The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. Laser Manipulation of Cells and Tissues ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build one's own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis - a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 13

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The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed to laser light during operations. The increased potential for exposure of humans highlights the need for scientifically-based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. We assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) (Xenometrix, Inc.) assay, it appears that 1064 nm, nanosecond pulses of laser light is sensed and induces several stress response genes, including p53, a gene in the DNA repair and apoptosis (cell suicide) regulatory pathways in a dose dependent fashion. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Book Description
The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed during operations. The increased potential for exposure of humans to laser light highlights the need for scientifically-based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. We assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) assay, it appears that 910 and 455 nm, femtosecond pulses of laser light is sensed and induces several stress response genes. Both wavelengths induced the xenobiotic receptor element in a roughly dose dependent fashion at 48 hours post exposure; while also inducing other genes to yield a genetic expression fingerprint unique to the exposure parameters. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.

Author: Jinze Qiu
Publisher:
ISBN:
Category :
Languages : en
Pages : 292

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The main purpose of this research was to investigate new medical applications of femtosecond laser ablation. A near-infrared femtosecond laser was tested and proved to be able to overcome the existing limitations and outperform the conventional long-pulse lasers in the areas of human urinary calculus (kidney stone) lithotripsy and skin treatment. The two primary objectives of my research are: 1) to investigate the feasibility of using femtosecond pulsed laser radiation to ablate urinary calculus of various compositions. The laser-calculus interaction mechanism was characterized using pump probe imaging and fast flash imaging. A novel fiber delivery system was developed to transmit and focus high energy femtosecond pulses for urinary calculus lithotripsy. The successful demonstration of the femtosecond laser lithotripsy provided a promising treatment method better than the existing long-pulse laser lithotripsy in a few different aspects, including less collateral damage to surrounding tissue, small-size debris and more controlled experimental condition. 2) to investigate the depth limitation of femtosecond subsurface ablation in scattering skin sample and develop a prototype tissue optical clearing device to enhance femtosecond beam penetration for deeper subsurface cavitation production in the skin. The successful demonstration of the device has potential benefits to new femtosecond-based therapies for reshaping or removing subcutaneous tissues.

Author: Takahiro Kasuya
Publisher: Routledge
ISBN:
Category : Cells
Languages : en
Pages : 96

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Book Description
First volume in a new series designed to provide information quickly on current as well as promising developments in lasers and consisting of self-contained tracts and handbooks pertinent to laser science and technology. Five color plates. Annotation copyrighted by Book News, Inc., Portland, OR

Author: Cheng Peng
Publisher:
ISBN: 9780542709418
Category :
Languages : en
Pages : 72

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Book Description

Author: Jan A. Nolta
Publisher: Springer
ISBN: 9789048169993
Category : Science
Languages : en
Pages : 0

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MSC (mesenchymal stem cells) have been reported to initiate revascularization after injury, to facilitate engraftment of blood-forming stem cells, and to reduce the incidence of graft-vs. host disease through their immune-suppressive qualities. Finally, bone marrow-derived MSC have been reported to home to areas of solid tumor revascularization, and thus may be used as delivery vehicles to target ablative agents into dividing tumor cells. Recently the characteristics of human MSC from adipose (fat) tissue have also been identified. The possibility of repairing tissues, speeding stem cell engraftment, and targeting solid tumors for specific killing, using MSC easily harvested from bone marrow, or better yet, from unwanted fat tissue, holds broad appeal, and is an intriguing possibility that could have dramatic effect on health care. This book has information on how to isolate, grow, and characterize MSC from marrow and fat, and gives important insight into how these cells may be used for gene delivery and cellular therapies in the future. Updates on emerging clinical trials are given.