Laboratory Techniques in Cellular and Molecular Medicine PDF Download

Author: Hajdúch, Marián
Publisher: Palacky University Olomouc
ISBN: 8024460491
Category : Medical
Languages : en
Pages : 436

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Book Description
The methodological book ""Laboratory techniques in cellular and molecular medicine"" is intended for students of bachelor, master, and doctoral study programmes at faculties of science, medicine, and veterinary medicine, as well as for laboratory technicians interested in methodological approaches of contemporary cellular and molecular medicine. The book does not aim to provide a comprehensive overview of the current state of the art in cellular and molecular medicine, that would be a superhuman task. The aim of the 56-member author team is to provide readers with an overview of the methods established and tested at the Institute of Molecular and Translational Medicine of the Faculty of Medicine of Palacký University Olomouc – to describe the methodological principles and their practical application. It focuses both on basic methods, whose principles are used by the most modern methods, and on special methods, reflecting the laboratory experience and specialisation of individual authors. The 52 chapters describe the work with cells and microorganisms, bioengineering manipulations of nucleic acids, the search for biomarkers, detection at the level of DNA, RNA, protein, organelle, and whole cell, and the use of fluorescent and radioactive labeling. To aid comprehension, the description of the methods is illustrated by figures and diagrams. Each chapter is followed by troubleshooting tips. The book promotes the best laboratory practice to increase the reproducibility of results in biomedicine.

Author: Heather B. Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 227

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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author: Anthony Contento
Publisher: Wiley Global Education
ISBN: 1118473957
Category : Science
Languages : en
Pages : 288

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Book Description
The Contento Experimental Cell Biology Lab Book is a modular design that matches the topics discussed in Karp’s textbook. The manual itself consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text. There are three possible designs of the lab book, based on the instructor’s needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs.This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.

Author: Morteza Jalali
Publisher: Academic Press
ISBN: 012803078X
Category : Science
Languages : en
Pages : 383

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Book Description
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Author: William R. Skach
Publisher: Springer Science & Business Media
ISBN: 1592591876
Category : Medical
Languages : en
Pages : 626

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Book Description
Since the cloning of the cystic fibrosis transmembrane conductance re- lator (CFTR) nearly a decade ago, cystic fibrosis (CF) research has witnessed a dramatic expansion into new scientific areas. Basic researchers, clinicians, and patients increasingly rely on fundamental techniques of genetics, molecular biology, electrophysiology, biochemistry, cell biology, microbiology, and immunology to understand the molecular basis of this complex disease. Research into the pathophysiology of CF has established numerous paradigms of ion channel dysfunction that extend from inflammation and infection in the airways of patients to basic mechanisms of protein processing and regulation in intracellular components. With these rapid advances has come an increasing need for research scientists to understand and utilize a growing array of basic laboratory tools. This volume of Methods in Molecular Medicine, Cystic Fibrosis Methods and Protocols satisfies that need by providing detailed protocols for the laboratory techniques used throughout CF research. From electrophysiology and cell biology, to animal models and gene therapy, the comprehensive set of methods covered here provide step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Contributions have been chosen to reflect the rich diversity of techniques and to provide a cohesive framework for understanding challenges that are currently at the forefront of CF research. It is hoped that this volume will serve as a valuable reference that will not only foster interdisciplinary investigations into current problems encountered in CF, but also facilitate the translation of new scientific discoveries into clinical solutions.

Author: Sean R. Gallagher
Publisher: John Wiley & Sons
ISBN: 047094241X
Category : Science
Languages : en
Pages : 679

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Book Description
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Author: Leland J. Cseke
Publisher: CRC Press
ISBN: 1420041711
Category : Medical
Languages : en
Pages : 598

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Book Description
Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

Author: Paul R. Selvin
Publisher: CSHL Press
ISBN: 087969775X
Category : Science
Languages : en
Pages : 511

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Book Description
Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

Author: Barry Mendelow
Publisher: Wits University Press
ISBN: 1868144658
Category : Science
Languages : en
Pages : 526

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Book Description
The insights following the wake of the Human Genome project are radically influencing our understanding of the molecular basis of life, health and disease. The improved accuracy and precision of clinical diagnostics is also beginning to have an impact on therapeutics in a fundamental way. This book is suitable for undergraduate medical students, as part of their basic sciences training, but is also relevant to interested under- and postgraduate science and engineering students. It serves as an introductory text for medical registrars in virtually all specialties, and is also of value to the General Practitioner wishing to keep up to date, especially in view of the growing, internet-assisted public knowledge of the field. There is a special focus on the application of molecular medicine in Africa and in developing countries elsewhere.

Author: Friedrich Lottspeich
Publisher: John Wiley & Sons
ISBN: 3527694463
Category : Science
Languages : en
Pages : 1810

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Book Description
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology