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Author: Matthew Cooper Publisher: John Wiley & Sons ISBN: 1119990270 Category : Medical Languages : en Pages : 357
Book Description
Over the past two decades the benefits of label-free biosensor analysis have begun to make an impact in the market, and systems are beginning to be used as mainstream research tools in many drug discovery laboratories. Label-Free Technologies For Drug Discovery summarises the latest and emerging developments in label-free detection systems, their underlying technology principles and end-user case studies that reveal the power and limitations of label-free in all areas of drug discovery. Label-free technologies discussed include SPR, NMR, high-throughput mass spectrometry, resonant waveguide plate-based screening, transmitted-light imaging, isothermal titration calorimetry, optical and impedance cell-based assays and other biophysical methods. The technologies are discussed in relation to their use as screening technologies, high-content technologies, hit finding and hit validation strategies, mode of action and ADME/T, access to difficult target classes, cell-based receptor/ligand interactions particularly orphan receptors, and antibody and small molecule affinity and kinetic analysis. Label-Free Technologies For Drug Discovery is an essential guide to this emerging class of tools for researchers in drug discovery and development, particularly high-throughput screening and compound profiling teams, medicinal chemists, structural biologists, assay developers, ADME/T specialists, and others interested in biomolecular interaction analysis.
Author: Matthew Cooper Publisher: John Wiley & Sons ISBN: 1119990270 Category : Medical Languages : en Pages : 357
Book Description
Over the past two decades the benefits of label-free biosensor analysis have begun to make an impact in the market, and systems are beginning to be used as mainstream research tools in many drug discovery laboratories. Label-Free Technologies For Drug Discovery summarises the latest and emerging developments in label-free detection systems, their underlying technology principles and end-user case studies that reveal the power and limitations of label-free in all areas of drug discovery. Label-free technologies discussed include SPR, NMR, high-throughput mass spectrometry, resonant waveguide plate-based screening, transmitted-light imaging, isothermal titration calorimetry, optical and impedance cell-based assays and other biophysical methods. The technologies are discussed in relation to their use as screening technologies, high-content technologies, hit finding and hit validation strategies, mode of action and ADME/T, access to difficult target classes, cell-based receptor/ligand interactions particularly orphan receptors, and antibody and small molecule affinity and kinetic analysis. Label-Free Technologies For Drug Discovery is an essential guide to this emerging class of tools for researchers in drug discovery and development, particularly high-throughput screening and compound profiling teams, medicinal chemists, structural biologists, assay developers, ADME/T specialists, and others interested in biomolecular interaction analysis.
Author: Ye Fang Publisher: Humana ISBN: 9781493949618 Category : Medical Languages : en Pages : 0
Book Description
This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab. Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.
Author: Tiantian Zhang Publisher: ISBN: Category : Languages : en Pages : 112
Book Description
This thesis presents a new technique, Transient Induced Molecular Electronic Spectroscopy (TIMES), to study biomolecular interaction without protein engineering or chemical modification. The TIMES method incorporates a unique design of microfluidic platform and integrated sensing electrodes, and is designed to operate in a label-free and immobilization-free manner to provide crucial information for protein-ligand interactions under relevant physiological conditions. We developed a physical model for the TIMES signal, and mathematically formulated the problem to attain physical insight of protein-ligand interactions without molecular probes, fluorescent labels, or immobilization of molecules. Using soft lithography and thin film technology, we designed and fabricated microfluidic based devices for the biomolecular detection. Incorporated with electronic amplification and signal processing, we achieved real time detection using the TIMES method. To demonstrate the functionality, accuracy and sensitivity of this method, we used the TIMES signals to find the dissociation constants for the affinity of reactions between several pairs of protein and ligand. Followed by experiments, we used simulations to prove the mechanism of TIMES method. Computational fluid dynamics (CFD) simulations were performed to demonstrate that the surface electric polarization signal originates from the induced image charges during the transition-state of surface mass transport, which is governed by the overall effects of protein concentration, hydraulic forces and surface fouling due to protein adsorption. Hybrid atomistic molecular dynamics (MD) simulations and free energy computations show ligand-binding affects protein structures and stability, producing different adsorption orientation and surface polarizations to generate characteristic TIMES signals. As a unique tool, TIMES offers a simple and effective method to investigate fundamental protein chemistry and drug discovery. Although the current work is focused on protein-ligand interactions, the TIMES method is a general technique that may be applied to study signals from reactions between many kinds of molecules.
Author: Joachim Wegener Publisher: Springer Nature ISBN: 3030324338 Category : Science Languages : en Pages : 286
Book Description
This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.
Author: Matthew A. Cooper Publisher: Cambridge University Press ISBN: 1139476785 Category : Science Languages : en Pages : 305
Book Description
Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis.
Author: Maximilian Oberleitner Publisher: Springer ISBN: 331945384X Category : Science Languages : en Pages : 391
Book Description
This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.
Author: William S. Kisaalita Publisher: CRC Press ISBN: 1420073508 Category : Medical Languages : en Pages : 404
Book Description
Advances in genomics and combinatorial chemistry during the past two decades inspired innovative technologies and changes in the discovery and pre-clinical development paradigm with the goal of accelerating the process of bringing therapeutic drugs to market. Written by William Kisaalita, one of the foremost experts in this field, 3D Cell-Based Bio
Author: Ramakrishna Seethala Publisher: CRC Press ISBN: 1420061690 Category : Medical Languages : en Pages : 504
Book Description
Building upon the foundation of basics discussed in the previous edition, the Second Edition provides a more in-depth look at the latest methods and technologies of advanced drug screening, an essential function of drug discovery. With extensively updated content and 21 new chapters, this text examines:quality and efficiency of drug target validati
Author: Ramakrishna Seethala Publisher: CRC Press ISBN: Category : Medical Languages : en Pages : 512
Book Description
A presentation of screening techniques, modern technologies, and high-capacity instrumentation for increased productivity in the development and discovery of new drugs, chemical compounds, and targeted delivery of pharmaceuticals. It contains practical applications and examples of strategies in cell-based and cell-free screens as well as homogeneous, fluorescence, chemiluminescence, and radioactive-based technologies.
Author: Matthew Cooper
Author: Matthew Cooper
Author: Ye Fang
Author: Tiantian Zhang
Author: Matthew A. Cooper
Author: Joachim Wegener
Author: Matthew A. Cooper
Author: Maximilian Oberleitner
Author: William S. Kisaalita
Author: Ramakrishna Seethala
Author: Ramakrishna Seethala