Isotope Techniques in Biochemistry PDF Download

Author: Norman Samuel Radin
Publisher:
ISBN:
Category : Biochemistry
Languages : en
Pages : 56

View

Book Description

Author: M.J. Unkovich
Publisher: Springer Science & Business Media
ISBN: 940159841X
Category : Science
Languages : en
Pages : 312

View

Book Description
In the last two decades technological advances in isotope ratio mass spectrometry have been very rapid, opening up new possibilities for analysis of biological and environmental materials. The new instrumentation has facilitated faster analysis of samples via automated sample preparation and multi-isotope analysis of single samples, resulting in considerable cost savings, and enabling access to isotope analysis for many more researchers. These changes are reflected in the rapidly growing international literature on stable isotopes. While there have been some excellent books and review papers aimed at interpreting isotope signals in biology and environmental science, there have been fewer attempts to provide practical tools for researchers making forays into this exciting new arena. This book aims to address this inadequacy by providing a set of practical guidelines for the application of a range of novel and well proven stable isotope techniques to the fields of plant physiological ecology, agriculture, marine ecology and palaeoecology. The book is the outcome of a weeklong workshop held under the auspices of the Cooperative Research Centre for Legumes in Mediterranean Agriculture (CLIMA 1992 - 2000) at The University of Western Australia and the CSIRO Floreat Laboratories, Perth, Western Australia, in February 1999. The workshop was designed to provide practical tools and experiences for researchers and students concerned with how one goes about using stable isotopes in field investigations.

Author:
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 52

View

Book Description

Author: David C. Coleman
Publisher: Academic Press
ISBN: 032315767X
Category : Science
Languages : en
Pages : 287

View

Book Description
Carbon Isotope Techniques deals with the use of carbon isotopes in studies of plant, soil, and aquatic biology. Topics covered include photosynthesis/translocation studies in terrestrial ecosystems; carbon relationships of plant-microbial symbioses; microbe/plant/soil interactions; and environmental and aquatic toxicology. Stable carbon isotope ratios of natural materials are also considered. Comprised of 15 chapters, this book begins with an introduction to radiation-counting instruments used in measuring the radioactivity in soil and plant samples containing carbon-14. The discussion then turns to the basic methods of 14C use in plant science, highlighted by three examples of applications in the field of plant physiology and ecology. Subsequent chapters explore the use of carbon isotope techniques for analyzing the carbon relationships of plant-microbial symbioses; the interactions of microbes, plants, and soils; and the degradation of herbicides and organic xenobiotics. Carbon dating and bomb carbon are also described. The final section is devoted to the uses and procedures for 13C and 11C. This monograph is intended for advanced undergraduate or graduate students, as well as generalist scientists who have not previously used radioisotopes or stable isotopes in their research.

Author: George Wolf
Publisher: Elsevier
ISBN: 1483225747
Category : Science
Languages : en
Pages : 184

View

Book Description
Isotope is Biology is a six-chapter supplementary text that covers the properties and application of isotopes as labels or analytical tools in biological research. The first chapters deal with the physico-chemical properties and radioactivity of isotopes. These chapters also explore their synthesis, preparation, radiation decomposition, and decay of radioactivity. The succeeding chapter considers other aspects of isotopes, including their effect of health, disposal, spills, and laboratory use. Another chapter examines the chemical and biochemical behavior, natural abundance, and the chemical stability of isotopic compounds. The final chapters describe several isotopic methods, namely, isotope dilution, paper chromatography, and autoradiography, with emphasis on their application in biological studies. This book will be of value to biologists, and graduate and undergraduate biology students.

Author: U.S. Atomic Energy Commission
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 48

View

Book Description
This bibliography contains 807 selected references on techniques for the use of radioactive and stable isotopes in biological sciences. These references were taken from the 1948-1956 open literature. A list of the journals from which they were selected and an author index are also included.

Author:
Publisher: Elsevier
ISBN: 0080924077
Category : Science
Languages : en
Pages : 324

View

Book Description
This book is the first laboratory manual to bring together basic procedures for measurement of stable and radioactive isotopes of nitrogen, with specific applications to plant, soil, and aquatic biology. This bench-top reference gives practical coverage of mass and emission spectrometry, nitrogen fixation, nitrification, and identification, organic nitrogen, and the radioactive isotope 13N. Methods are described so that researchers can adapt them, without the aid of outside references, to virtually any task they may encounter in investigations of nitrogen transformation processes. Serves as a practical guide for nitrogen isotope techniques Features studies of nitrogen transformations in terrestrial and aquatic systems Includes basic measurement techniques plus specific applications for stable and radioactive nitrogen isotopes Presents detailed protocols, overviews, and key references Includes fifty figures and sixteen tables Hands-on reference for both students and researchers

Author: Robert J. Slater
Publisher: Practical Approach Series
ISBN: 9780199638260
Category : Medical
Languages : en
Pages : 332

View

Book Description
Provides an introduction to the use of radioactivity in the bioscience laboratory. The text covers general aspects of radioactivity, methods for the detection of radioactivity, radioisotope protocols used to study key cellular processes, and a summary of legislative requirements in the US and European Union. Guidance on safe handling and detailed recipes are provided.

Author: R.M. Lambrecht
Publisher: Springer
ISBN:
Category : Mathematics
Languages : en
Pages : 534

View

Book Description
These lectures were prepared by the authors for Seminars to be held on June 6, 1983, in St.Louis, Missouri, under the spon­ sorship of the Radiopharmaceutical Science Council of the So­ ciety of Nuclear Medicine . All manuscript? were refereed . Tracer kinetics and the modeling of physiological and bio­ chemical processes in vivo are the focus of a contemporary di­ rection in biochemical research. Recent advances in instrumen­ tation (especially positron emission tomography and digital autoradiography) and parallel developments in the production of short-lived radionuclides and rapid synthetic chemistry to pre­ pare tracers that probe metabolism, flow, receptor-ligand kinetics, etc., are responsible for new scientific frontiers. These developments, coupled with biomathematics and computer science, make it possible to quantitatively evaluate tracer kinetic models in animals and man. (The choice of animal models in radiotracer design and tracer kinetics is the subject of a book edited by R.M.Lambrecht and W.C.Eckleman in press at Sprin­ ger Verlag.) Tracer kinetics and physiological modeling is truly multi­ disciplinary, as evidenced by the intellectual diversity and international representation observable in the list of contri­ butors . The lectures outline and attempt to show the transition from the theoretical description to the practical application of modeling for understanding normal and pathological processes .

Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 0521516358
Category : Medical
Languages : en
Pages : 769

View

Book Description
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.