Inheritance and Quantitative Trait Loci Analysis of Leaf Rust Resistance in Wheat PDF Download

Author: Julia Xiuling Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 226

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Author: Xianming Chen
Publisher: Springer
ISBN: 9402411119
Category : Science
Languages : en
Pages : 723

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This book comprehensively introduces stripe rust disease, its development and its integral control. Covering the biology, genetics, genome, and functional genomics of the pathogen, it also discusses host and non-host resistance, their interactions and the epidemiology of the disease. It is intended for scientists, postgraduates and undergraduate studying stripe rust, plant pathology, crop breeding, crop protection and agricultural science, but is also a valuable reference book for consultants and administrators in agricultural businesses and education.

Author: Izabela L. Ciechanowska
Publisher:
ISBN:
Category : Wheat
Languages : en
Pages : 0

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Marker-assisted selection requires the identification of molecular markers associated with major genes and quantitative trait loci (QTL) using linkage analysis. In this study, we used 167 doubled haploid (DH) lines derived from two unregistered spring wheat (Triticum aestivum L.) parental lines that belong to the Canada Western Special Purpose (CWSP) class to map QTLs associated with five traits using inclusive composite interval mapping (ICIM). Using ICIM, least square means phenotype data across 3-4 environments, and a genetic map of 2,676 SNPs out of the wheat 90K SNP array, we identified ten QTLs associated with maturity (4A and 5B), plant lodging (4B, 5A, 5D, and 7D), grain yield (2D), leaf rust (4A) and stem rust (1A and 2B). Each QTL individually accounted for 6.0-22.3% of the phenotypic variance and together accounted for 8.6-38.2% of each trait. QTLs identified for rusts using ICIM had a minor effect (6.0-9.0%) or a major effect (22.3%). Our major effect QTL at 22.3% was discovered on chromosome 2B and contributed to stem rust response. Its physical location has been associated with disease response in previous studies. Results from this study provide additional valuable information to wheat researchers, in particular that the area on chromosome 2B should be considered for future analyses.

Author: Bhoja Basnet
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Wheat (Triticum aestivum L.) is one of the most important food crops, comprising the largest source of daily calorie and protein intake of human beings worldwide. Among the several diseases of wheat, Yellow Rust (YR; caused by Puccinia striiformis Westend. f. sp. tritici) and Leaf Rust (LR; caused by Puccinia triticina Erikss. & Henn.) have always been major production constraints since the domestication of wheat. For the last few decades, scientists have invested large efforts to identify, characterize and utilize Adult Plant Resistance (APR), a.k.a. slow rusting resistance, in wheat germplasm to promote durability of resistance against rust. The objectives of this study were to 1) understand the genetics of APR to YR and/or LR present in two potential wheat lines 'Quaiu 3' and 'TAM 111', and 2) map the putative Quantitative Trait Loci (QTL) associated with YR and LR resistance using DNA-based molecular markers. Two Recombinant Inbred Line (RIL) populations were subjected to YR and LR disease evaluation experiments in multiple years and locations. Visual evaluation of Disease severity (DS) and Infection Type (IT) score in both RIL populations showed that APR to YR and LR were highly heritable quantitative traits with significant correlation among experiments. In spring wheat population, composite interval mapping consistently detected four and three large effect QTL for YR and LR resistance, respectively. Among those QTLs, 1B, 3B and 1D QTL were found to be associated with previously characterized genes Lr46/Yr29, Sr2/Yr30 and Lr42, respectively. However, QTLs QYr.tam-3D and QYr.tam-2D were potentially novel. The largest YR QTL QYr.tam-2D was located on long arm of chromosome 2D explaining about 48 to 61% of the total phenotypic variation. Similarly, in winter wheat population, apart from three environment-specific QTL on chromosomes 1A, 2A and 7D, the QTL on chromosome 2B (QYr.tam-2B) was found to express consistently in multiple environments explaining about 23 to 63% of total phenotypic variation. This study has further elucidated the inheritance mechanism of APR to YR and LR present in two different wheat lines, Quaiu 3 and TAM 111, and resulted in the successful mapping and characterization of the genetic loci associated with corresponding disease resistance traits. These findings should be very useful to isolate the novel APR genes and/or directly use in wheat breeding programs to enhance durable rust resistance in diverse wheat germplasm and cultivars in the future.

Author: Yue Lu
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Wheat leaf rust, caused by Puccinia triticina, is an important fungal disease worldwide. Growing resistant cultivars is an effective practice to reduce the losses caused by the disease, and using slow-rusting resistance genes can improve the durability of rust resistance in the cultivars. CI13227 is a winter wheat line that shows a high level of slow-rusting resistance to leaf rust and has been studied extensively. In this research, two recombinant inbreed line (RIL) populations derived from CI13227 x Suwon (104 RILs) and CI13227 x Everest (184 RILs) and one doubled haploid (DH) population derived from CI13227 x Lakin with 181 lines were used to identify quantitative trait loci (QTLs) for slow leaf rusting resistance. Each population and its parents were evaluated for slow-rusting traits in two greenhouse experiments. A selected set of 384 simple sequence repeat markers (SSRs), single nucleotide polymorphism markers (SNPs) derived from genotyping-by-sequencing (GBS-SNPs) or 90K-SNP chip (90K-SNPs) were analyzed in the three populations. Six QTLs for slow-rusting resistance, QLr.hwwgru-2DS, QLr.hwwgru-7BL, QLr.hwwgru-7AL, QLr.hwwgru-3B_1, QLr.hwwgru-3B_2, and QLr.hwwgru-1D were detected in the three populations with three stable QTLs, QLr.hwwgru-2DS, QLr.hwwgru-7BL and QLr.hwwgru-7AL. These were detected and validated by Kompetitive Allele-Specific PCR (KASP) markers converted from GBS-SNPs and 90K-SNPs in at least two populations. Another three QTLs were detected only in a single population, and either showed a minor effect or came from the susceptible parents. The KASP markers tightly linked to QLr.hwwgru-2DS (IWB34642, IWB8545 and GBS_snpj2228), QLr.hwwgru-7BL (GBS_snp1637 and IWB24039) and QLr.hwwgru-7AL (IWB73053 and IWB42182) are ready to be used in marker-assisted selection (MAS) to transfer these QTLs into wheat varieties to improve slow-rusting resistance in wheat.

Author: International Maize and Wheat Improvement Center
Publisher: CIMMYT
ISBN: 9789686127232
Category : Technology & Engineering
Languages : en
Pages : 164

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Author: Z. Eyal
Publisher: CIMMYT
ISBN: 9789686127065
Category : Septoria
Languages : en
Pages : 56

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Author: Joseph Hahn
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Leaf rust, caused by the fungus Puccinia triticina, is a major disease of wheat in North America. Breeding efforts to achieve resistance have historically been plagued by a cycle in which an all-stage resistance gene is discovered, deployed, and rapidly defeated, thus wasting valuable sources of resistance. Adult-plant resistance genes, however, can potentially contribute to race-nonspecific resistance to the pathogen which is durable for many years. In this study, the moderately susceptible Kansas winter wheat cultivar, 'Lakin', was backcrossed to the CIMMYT-developed spring wheat cultivar, 'Roelfs F2007', which is known to possess very good adult-plant resistance (APR) to leaf rust. The 297 BC1F6-derived progeny of this cross were screened for resistance in five location-years at field nurseries in Kansas and Texas, as well as in two greenhouse studies. Genotyping-by-Sequencing (GBS) generated 2,541 high-quality genome-wide markers used for the construction of a linkage map covering all 21 chromosomes. Quantitative trait locus (QTL) analysis identified reproducible APR QTLs on chromosome arms 3BS, 5DS, 7BS, and 7BL. Three QTLs contributed by Roelfs F2007 appeared to correspond to locations of known APR genes Lr74 (3BS), Lr78 (5DS), and Lr68 (7BL). The APR QTL on 7BS contributed by Lakin appeared to be novel. Together, the four QTLs accounted for 45% and 46% of the variation in least-squares means for disease severity and infection type, respectively. These results confirm that both Roelfs F2007 and Lakin contain APR loci which may be used in breeding for resistance to infections by P. triticina.

Author: Peter Michael Bulli Nyori
Publisher:
ISBN:
Category :
Languages : en
Pages :

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In this research, a recombinant inbred line (RIL) population derived from cross between a leaf rust- and stripe rust-susceptible spring wheat 'Avocet S' and a slow leaf- and stripe-rusting resistant spring wheat 'Amadina' was used to postulate and map leaf rust seedling resistance genes, identify quantitative trait loci (QTL) for slow-rusting resistance against leaf and stripe rust, and study slow leaf-rusting components, latent period and infection frequency. Two known Lr genes (Lr23, and Lr26) were identified to be present in 'Amadina' through gene postulation, pedigree, cytogenetic, and polymerase chain reaction analyses. One unknown gene associated with seedling resistance was also mapped on chromosome 1BL. In greenhouse experiment, it was estimated that at least five genes conditioning final disease severity (FS) and latent period (LP), and four genes conditioning infection frequency (IF), segregated in the population. Correlations between LP and FS, and LP and IF were moderately negative, and that between IF and FS was moderately positive, indicating inter-dependence of the traits. Two QTL on chromosomes 1BL and 6BL were associated with LP and FS, and three QTL on chromosomes 1BL, 6BL and 2DS were associated with IF. Segregation of the RIL population in field experiment indicated that there were at least four and three adult plant resistance (APR) genes involved in resistance for leaf and stripe rust. Six QTL on chromosomes 3AL, 4AL, 1BL, 5BL, and 7BL were associated with APR for leaf rust, and seven QTL on chromosome 4AL, 5AL, 1BL, 2BL, 4BL, 5BL, 2DL, and 4D were associated with APR for stripe rust. Our results indicated that the major portion of genetic variability for slow-rusting resistance was additive gene action, and, to some extent, epistasis. In this research, we also explored the utility of remote sensing and geographic information systems (GIS) and analytical operations to discriminate leaf rust pustules from other parts of leaf and to accurately determine pustule size in 'Amadina' and 'Avocet S'.

Author: Xiangyu (Bruce) Pei
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Leaf rust, caused by Puccinia triticina Eriks. (Pt = P. recondita Rob. Ex Desmaz. F. sp. tritici), is an economically significant pathogen affecting durum wheat (Triticum turgidum var. durum L.) crops worldwide. Genetic resistance is one of the most effective and environmentally friendly methods to control leaf rust in wheat. New sources of resistance genes need to be identified due to the threat of new Pt races to durum wheat production. Marker assisted selection (MAS) is a highly efficient method to select resistance genes in breeding programs particularly to pyramid multiple resistance genes in new varieties. The objective of this study was to characterize and map leaf rust resistance genes in a Canadian durum wheat Strongfield. A double haploid (DH) mapping population of 87 DH lines was developed from the cross Strongfield/Blackbird. Seedling rust tests with Pt isolates 12-3 MBDS, 06-1-1 TDBG, 128-1 MBRJ, 74-2 MGBJ, and 77-2 TJBJ revealed a single hypersensitive leaf rust resistance gene. Three genes segregated for resistance to isolate 1-1 BBBD at the seedling stage, one of which controlled resistance to the other five Pt isolates. Blackbird contributed one of the seedling resistance genes effective against isolate 1-1 BBBD. Parental lines and 87 DH lines were genotyped using the Illumina Infinium assay with the iSelect 90K wheat SNP array. A database search using the DNA sequences of linked markers provided a putative location in the Chinese Spring reference genome sequence. The gene conferring resistance to the six isolates used in the study mapped to the long arm of chromosome 3A and was temporarily designated as LrStr_3A. No leaf rust resistance gene has been detected in this region previously. Quantitative trait locus (QTL) analysis identified eight QTL controlling leaf rust resistance in field leaf rust nurseries. One of these QTL mapped to chromosome 3AL as the same region of LrStr_3A. Another QTL mapped to the expected location of the adult plant resistance (APR) gene Lr46 based upon the marker csLV46G22. Kompetitive allele-specific PCR (KASP) markers were developed for LrStr_3A that will be useful for MAS.