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Author: P.A. Baeuerle Publisher: Springer Science & Business Media ISBN: 1468468405 Category : Medical Languages : en Pages : 294
Book Description
Cells have evolved multiple strategies to adapt the composition and quality of their protein equipment to needs imposed by changes in intra- and extracellular conditions. The appearance of pro teins transmit ting novel functional properties to cells can be controlled at a transcrip tional, posttranscriptional, translational or posttranslational level. Extensive research over the past 15 years has shown that transcriptional regulation is used as the predominant strategy to control the production of new proteins in response to extracellular stimuli. At the level of gene transcription, the initiation ofmRNA synthesis is used most frequently to govern gene expression. The key elements controlling transcription initiation in eukaryotes are activator proteins (transactivators) that bind in a sequence-specific manner to short DNA sequences in the of genes. The activator binding sites are elements of larger proximity control units, ca lied promoters and enhancers, which bind many distinct proteins. These may synergize or negatively cooperate with the activators. The do novo binding of an activator to DNA or, if already bound to DNA, its functional activation is what ultimately turns on a high-level expression of genes. The activity of transactivators is controlled by signalling pathways and, in some cases, transactivators actively partici pate in signal transduction by moving from the cytoplasm into the nuc1eus. In this first volume of Inducible Gene Expression, leading scientists in the field review six eukaryotic transactivators that allow cells to respond to various extracellular stimuli by the expression of new proteins.
Author: P.A. Baeuerle Publisher: Springer Science & Business Media ISBN: 1468468405 Category : Medical Languages : en Pages : 294
Book Description
Cells have evolved multiple strategies to adapt the composition and quality of their protein equipment to needs imposed by changes in intra- and extracellular conditions. The appearance of pro teins transmit ting novel functional properties to cells can be controlled at a transcrip tional, posttranscriptional, translational or posttranslational level. Extensive research over the past 15 years has shown that transcriptional regulation is used as the predominant strategy to control the production of new proteins in response to extracellular stimuli. At the level of gene transcription, the initiation ofmRNA synthesis is used most frequently to govern gene expression. The key elements controlling transcription initiation in eukaryotes are activator proteins (transactivators) that bind in a sequence-specific manner to short DNA sequences in the of genes. The activator binding sites are elements of larger proximity control units, ca lied promoters and enhancers, which bind many distinct proteins. These may synergize or negatively cooperate with the activators. The do novo binding of an activator to DNA or, if already bound to DNA, its functional activation is what ultimately turns on a high-level expression of genes. The activity of transactivators is controlled by signalling pathways and, in some cases, transactivators actively partici pate in signal transduction by moving from the cytoplasm into the nuc1eus. In this first volume of Inducible Gene Expression, leading scientists in the field review six eukaryotic transactivators that allow cells to respond to various extracellular stimuli by the expression of new proteins.
Author: Chris Voigt Publisher: Academic Press ISBN: 0123850762 Category : Science Languages : en Pages : 733
Book Description
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation
Author: Masayori Inouye Publisher: Academic Press ISBN: 1483273970 Category : Science Languages : en Pages : 330
Book Description
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
Author: P.A. Baeuerle Publisher: Springer Science & Business Media ISBN: 1468468375 Category : Medical Languages : en Pages : 293
Book Description
This is the first of two volumes which aim to cover all established eukaryotic transcription factor systems that are direct targets for the signal transduction pathways. Leading molecular biologists contribute reviews on topics which take a broad view, and which should be of interest to students and advanced researchers in biology and medicine. Chapters attempt to answer such fundamental questions as: what is the signal and how and when is it produced? What is the receptor, and what further signalling molecules are involved? What is the biochemistry and molecular biology of the transcription factor that is the ultimate target of the signalling pathway? What is the physiological role of factor?
Author: Joseph M. Fernandez Publisher: Academic Press ISBN: Category : Science Languages : en Pages : 504
Book Description
Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (~2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products. Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs.
Author: Gerald R. Reeck Publisher: Springer Science & Business Media ISBN: 1468476157 Category : Science Languages : en Pages : 359
Book Description
This book stems from an Advanced Study Institute on Chromo somal Proteins and Gene Expression that was held in Sitges, Spain, on September 17-26, 1984. It would be misleading to call this volume a conference proceedings, however. The ASI was not a conference, but a course with diverse activities, only one of which was a set of major presentations by the lecturers. Indeed, the concept of lecturer was intentionally obscured as we all learned from each other through shorter presentations by other participants and through seminars, poster sessions, and small group discussions. Furthermore, many participants found that exchanging ideas outside organized sessions was among the most rewarding aspects of the course. Some even claimed to have profitably probed the intricacies of nucleosome structure and transcriptional regulation while basking in the sun on the beachl Obviously, it is difficult to catch the flavor of such varied proceedings in a book. (I cannot confirm the incident on the beach, never having found time to set foot there. Such is the fate of the director of a meeting. ) The ASI was judged a success -- and enthusiastically so - by most participants. Not only did we deepen our understanding of our scientific field, we made new friends and learned about scientific and nonscientific aspects of life in other countries and about issues that transcend international boundaries in our complex world. We hope that this volume will be as successful as the course was.
Author: Robert C. Roach Publisher: Springer Science & Business Media ISBN: 9780306466960 Category : Medical Languages : en Pages : 466
Book Description
Hypoxia remains a constant threat throughout life. It is for this reason that the International Hypoxia Society strives to maintain a near quarter century tradition of presenting a stimulating blend of clinical and basic science discussions. International experts from many fields have focused on the state-of-the-art discoveries in normal and pathophysiological responses to hypoxia. Topics in this volume include gene-environment interactions, a theme developed in both a clinical context regarding exercise and hypoxia, as well as in native populations living in high altitudes. Furthermore, experts in the field have combined topics such as skeletal muscle angiogenesis and hypoxia, high altitude pulmonary edema, new insights into the biology of the erythropoietin receptor, and the latest advances in cardiorespiratory control in hypoxia. This volume explores the fields of anatomy, cardiology, biological transport, and biomedical engineering among many others.
Author: Paul H. S. Reynolds Publisher: Cabi ISBN: Category : Science Languages : en Pages : 264
Book Description
This book reviews the latest information on the wide range of promoter systems which have been developed in order to obtain external, inducible control of gene expression in plants. In twelve chapters the systems which are currently in use are surveyed and their individual strengths and weaknesses are analysed. Future developments in this exciting area of research are foreshadowed. Written by experts from North America, New Zealand, Europe and Japan, this book will be essential reading for biotechnologists and will be of interest to all researchers and graduate students in plant genetics and molecular biology.
Author: Lutz Nover Publisher: CRC Press ISBN: 9780849349126 Category : Science Languages : en Pages : 538
Book Description
This book focuses on heat shock response-an active yet transient reprogramming of cellular activities to the needs of a stress protection mechanism designed to minimize heat damage and to optimize restoration of normal cellular activities after the stress period. This work places major emphasis on the structure and possible cellular functions of heat shock proteins as well as the analysis of heat shock protein-coding genes by transfection into homologous and heterologous expression systems. It also discusses heat shock effects on all levels of gene expression, on cell ultrastructure, and metabolic activities. This unique text is a must for all those who are involved with genetics, nucleic acids research, and cancer research.
Author: Ann Arvin Publisher: Cambridge University Press ISBN: 1139461648 Category : Medical Languages : en Pages : 1325
Book Description
This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Author: P.A. Baeuerle
Author: P.A. Baeuerle
Author: Chris Voigt
Author: Masayori Inouye
Author: P.A. Baeuerle
Author: Joseph M. Fernandez
Author: Gerald R. Reeck
Author: Robert C. Roach
Author: Paul H. S. Reynolds
Author: Lutz Nover
Author: Ann Arvin