In Vitro Fertilizing Characteristics of Bovine Sperm with Abnormal Morphology PDF Download
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Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
A combination of in vitro fertilization (IVF) and sperm function tests were used to determine the fertilizing characteristics of bovine sperm with abnormal morphology. Bovine sperm affected with the pyriform defect had a reduced ability to bind to and penetrate zonae pellucidae and although those penetrating oocytes were involved in the fertilization process, the resulting zygotes appeared to have a reduced ability to initiate cleavage. Bovine sperm with the knobbed acrosome defect had a reduced ability to bind to and penetrate the zona pellucida. Knobbed sperm had impaired sperm plasma membrane function which predisposed them to spontaneous acrosome reaction on incubation after thawing, reducing oocyte interaction. The post-zona penetration fertilizing characteristics of normal sperm associated with knobbed sperm also appeared to be impaired. Bovine sperm with multiple nuclear vacuoles had a reduced ability to bind to and penetrate the zona pellucida of bovine oocytes. However, those gaining access to the ooplasm participated normally in fertilization. A proportion of sperm with proximal droplets failed to swim through the sodium hyaluronate swim-up medium and sperm with proximal droplets failed to bind with the zona pellucida of oocytes. Apparently normal sperm co-existing in the semen along with proximal droplets were also functionally deficient, resulting in reduced zonae binding, and those oocytes that were fertilized did not develop beyond the cleavage stage of development. In summary, morphologically abnormal sperm failed during the various steps of the fertilization process depending on the nature of abnormality. However, knobbed sperm, pyriform-shaped sperm, sperm with multiple nuclear vacuoles and sperm with proximal cytoplasmic droplets appeared to have similar functional deficiencies suggesting that abnormal morphology may be a marker of accompanying functional deficiencies. The normal sperm coexisting in the semen along with high proportions of abnormal.
Author: Publisher: ISBN: Category : Languages : en Pages :
Book Description
A combination of in vitro fertilization (IVF) and sperm function tests were used to determine the fertilizing characteristics of bovine sperm with abnormal morphology. Bovine sperm affected with the pyriform defect had a reduced ability to bind to and penetrate zonae pellucidae and although those penetrating oocytes were involved in the fertilization process, the resulting zygotes appeared to have a reduced ability to initiate cleavage. Bovine sperm with the knobbed acrosome defect had a reduced ability to bind to and penetrate the zona pellucida. Knobbed sperm had impaired sperm plasma membrane function which predisposed them to spontaneous acrosome reaction on incubation after thawing, reducing oocyte interaction. The post-zona penetration fertilizing characteristics of normal sperm associated with knobbed sperm also appeared to be impaired. Bovine sperm with multiple nuclear vacuoles had a reduced ability to bind to and penetrate the zona pellucida of bovine oocytes. However, those gaining access to the ooplasm participated normally in fertilization. A proportion of sperm with proximal droplets failed to swim through the sodium hyaluronate swim-up medium and sperm with proximal droplets failed to bind with the zona pellucida of oocytes. Apparently normal sperm co-existing in the semen along with proximal droplets were also functionally deficient, resulting in reduced zonae binding, and those oocytes that were fertilized did not develop beyond the cleavage stage of development. In summary, morphologically abnormal sperm failed during the various steps of the fertilization process depending on the nature of abnormality. However, knobbed sperm, pyriform-shaped sperm, sperm with multiple nuclear vacuoles and sperm with proximal cytoplasmic droplets appeared to have similar functional deficiencies suggesting that abnormal morphology may be a marker of accompanying functional deficiencies. The normal sperm coexisting in the semen along with high proportions of abnormal.
Author: Albert Barth Publisher: Springer ISBN: 9783031701252 Category : Science Languages : en Pages : 0
Book Description
This book provides a broad perspective on understanding bovine fertility, focusing on the classification and interpretation of bovine sperm defects. Building upon the success of its first edition published in 1989, this new edition has been significantly updated and expanded to reflect developments over the past three decades. The chapters cover topics such as the normal and abnormal development of bovine sperm, the mechanisms behind sperm defects, and the impact of these defects on fertility. Special attention is drawn at advances in genomic research and the use of sperm quality biomarkers and genetic screening tests in the assessment of bull fertility. In addition to this, the work explores critical periods for sexual development in bulls, such as early gestation and pre-weaning. This monograph is intended for researchers and students in the field of animal reproduction, veterinarians, and animal scientists. It provides a deep understanding of bovine fertility, a topic that is crucial for anyone working in livestock production or related fields. The knowledge gained from this book will be beneficial to those seeking to improve livestock productivity and thus contribute to global food security.
Author: Larry I. Lipshultz Publisher: Cambridge University Press ISBN: 1139483242 Category : Medical Languages : en Pages : 691
Book Description
The new edition of this canonical text on male reproductive medicine will cement the book's market-leading position. Practitioners across many specialties - including urologists, gynecologists, reproductive endocrinologists, medical endocrinologists and many in internal medicine and family practice – will see men with suboptimal fertility and reproductive problems. The book provides an excellent source of timely, well-considered information for those training in this young and rapidly evolving field. While several recent books provide targeted 'cookbooks' for those in a male reproductive laboratory, or quick reference for practising generalists, the modern, comprehensive reference providing both a background for male reproductive medicine as well as clinical practice information based on that foundation has been lacking until now. The book has been extensively revised with a particular focus on modern molecular medicine. Appropriate therapeutic interventions are highlighted throughout.
Author: Richard M. Hopper Publisher: John Wiley & Sons ISBN: 1118470850 Category : Medical Languages : en Pages : 2206
Book Description
Bovine Reproduction is a comprehensive, current reference providing information on all aspects of reproduction in the bull and cow. Offering fundamental knowledge on evaluating and restoring fertility in the bovine patient, the book also places information in the context of herd health where appropriate for a truly global view of bovine theriogenology. Printed in full color throughout, the book includes 83 chapters and more than 550 images, making it the most exhaustive reference available on this topic. Each section covers anatomy and physiology, breeding management, and reproductive surgery, as well as obstetrics and pregnancy wastage in the cow. Bovine Reproduction is a welcome resource for bovine practitioners, theriogenologists, and animal scientists, as well as veterinary students and residents with an interest in the cow.
Author: Leslie C. Sharkey Publisher: John Wiley & Sons ISBN: 1119125723 Category : Medical Languages : en Pages : 1024
Book Description
This book offers a state-of-the-art, evidence-based reference to all aspects of veterinary cytology. Truly multidisciplinary in its approach, chapters are written by experts in fields ranging from clinical pathology to internal medicine, surgery, ophthalmology, and dermatology, drawing the various specialties together to create a comprehensive picture of cytology's role in diagnosis and treatment of animal disease. Firmly grounded in the primary literature, the book focuses on companion animals, with special chapters for species with fewer publications. Chapters are logically organized by body system, with additional chapters on tumors of particular import and diagnostic decision making. The first two sections of Veterinary Cytology focus on cytology techniques, quality control, and special laboratory techniques. Subsequent sections are organ/tissue-based and reflect what is known about the canine, feline, and equine species. This is followed by chapters on non-traditional species, including exotic companion mammals, rabbits, cattle, camelids, non-human primates, reptiles and birds, amphibians, fish, invertebrates, and sheep and goats. The last section highlights some unique features of the applications of cytology in industry settings. Provides a gold-standard reference to data-driven information about cytologic analysis in companion animal species Brings together authors from a wide range of specialties to present a thorough survey of cytology's use in veterinary medicine Offers broader species coverage and greater depth than any cytology reference currently available Veterinary Cytology is an essential resource for clinical and anatomic pathologists and any specialist in areas using cytology, including veterinary oncologists, criticalists, surgeons, ophthalmologists, dermatologists, and internists.
Author: Patrick Fénichel Publisher: John Libbey Eurotext ISBN: 9782742000944 Category : Medical Languages : en Pages : 518
Book Description
This valuable reference work provides a synthesis of the latest knowledge about the acrosomatic reaction of human sperm. The acrosomatic reaction, which is a fundamental step in the process of fertilisation, is examined on both the fundamental and clinical levels. Three very recent aspects are also dealt with: what happens to the acrosome during intracytoplasmic microinjections, the usefulness of screening spermatozoa that have completed their acrosomatic reaction, and the development of contraception by a vaccine that targets one of the proteins of the acrosome. International Symposium, Collioure, France, September 1995.
Author: Rita Payan-Carreira Publisher: BoD – Books on Demand ISBN: 9535134779 Category : Medical Languages : en Pages : 194
Book Description
Theriogenology, the field that studies animal reproductive health and disease, is a challenging field that shows a steady growth. It covers diverse aspects of reproduction in domestic and wild animals, including the assisted reproductive techniques, which have enormously enhanced the ability to rescue endangered species and provide a strong support to the high reproductive efficiency requested by livestock production. Reproductive success, as well as infertility, is the culmination of complex physiological and adaptive processes that guarantee, at the end, a species' ability to reproduce and its survival in a challenging and ever-changing environment. In this book, we present to you a collection of manuscripts exploring various aspects of the reproductive function of mammal and marine species. I hope you find this a useful book in your collection.
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Author: A. D. Barth
Author: Albert Barth
Author: Larry I. Lipshultz
Author: Richard M. Hopper
Author: World Health Organisation
Author: Leslie C. Sharkey
Author: Patrick Fénichel
Author: Rita Payan-Carreira