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Author: M. A. Hayat Publisher: CRC Press ISBN: 9780849324499 Category : Science Languages : en Pages : 344
Book Description
This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: M. A. Hayat Publisher: CRC Press ISBN: 9780849324499 Category : Science Languages : en Pages : 344
Book Description
This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: Gerhard W. Hacker Publisher: CRC Press ISBN: 1420040235 Category : Medical Languages : en Pages : 265
Book Description
Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a
Author: Alexander D. Hyatt Publisher: CRC Press ISBN: 9780849367595 Category : Science Languages : en Pages : 476
Book Description
This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.
Author: M. A. Hayat Publisher: CRC Press ISBN: 1040290531 Category : Science Languages : en Pages : 337
Book Description
This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: Publisher: Elsevier ISBN: 0323138810 Category : Nature Languages : en Pages : 565
Book Description
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. - Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry - Methods for preparing colloidal gold particles of different sizes - Protein A-gold, protein G-gold, and lectin-gold techniques - The use of resins and thin cryosections - Multiple labeling
Author: Tadao Sugimoto Publisher: Elsevier ISBN: 0444627502 Category : Science Languages : en Pages : 852
Book Description
Monodispersed Particles, Second Edition, covers all aspects of monodispersed particles, including inorganic and polymer particles and their composites. The book consists of four parts: fundamentals, preparation, analyses, and applications. Specifically, the fundamental part covers the theoretical insight into the surface energy of particles and its application to the formulation of the new theories of nucleation, growth and habit formation of monodispersed particles. In addition, the theories of recrystallization and solid-solution formation are introduced. These fundamental theories are applied to the precise control of their size, size distribution, shape, internal structure, and composition, leading to the design of diverse monodispersed functional particles widely used in basic science and modern industry.This second edition is fully updated and revised, detailing new theories and recent progress in the field of nanoparticles, including advanced nucleation theory, arrested growth mechanism for monodispersed nanoparticles, and energetics of habit formation. Additionally, the text covers in-depth insights into the anisotropic growth of Au and Ag nanoparticles, the formation mechanisms of polycrystalline Au spheres, iron oxide nanoparticles in heat-up and hot-injection processes, amorphous TiO2 spheres in a sol-gel system, anatase TiO2 in a gel-sol system and their shape control, AgCl nanoparticles in a reverse micelle system, organic-inorganic hybrid liquid crystals, and extensive biomedical applications. - Covers most of the known uniform particles, including inorganic and polymer particles and their composites - Includes development of novel fundamental theories of formation mechanisms, full of the author's own original ideas, and detailed background discussion on recent progress in the feild of nanoparticles and the latest advances in their applications - Features 2000 bibliographic references, providing a comprehensive guide to related study
Author: John Pound Publisher: Springer Science & Business Media ISBN: 1592592570 Category : Science Languages : en Pages : 501
Book Description
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Author: John M. Walker Publisher: Springer ISBN: 1592591698 Category : Science Languages : en Pages : 1099
Book Description
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Author: Stefan Lorkowski Publisher: John Wiley & Sons ISBN: 3527605339 Category : Science Languages : en Pages : 992
Book Description
This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Author: M. A. Hayat
Author: M. A. Hayat
Author: Gerhard W. Hacker
Author: Jiang Gu
Author: Alexander D. Hyatt
Author: M. A. Hayat
Author: 
Author: Tadao Sugimoto
Author: John Pound
Author: John M. Walker
Author: Stefan Lorkowski