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Author: Colin R. Phillips Publisher: Springer Science & Business Media ISBN: 3642732496 Category : Science Languages : en Pages : 171
Book Description
Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .
Author: Colin R. Phillips Publisher: Springer Science & Business Media ISBN: 3642732496 Category : Science Languages : en Pages : 171
Book Description
Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .
Author: Ichiro Chibata Publisher: Elsevier ISBN: 1483215865 Category : Science Languages : en Pages : 372
Book Description
Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Author: Jose M. Guisan Publisher: Humana ISBN: 9781493959884 Category : Science Languages : en Pages : 0
Book Description
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Author: Jose M. Guisan Publisher: Humana ISBN: 9781071602171 Category : Science Languages : en Pages : 495
Book Description
This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Author: Mattiasson Publisher: CRC Press ISBN: 1351081845 Category : Science Languages : en Pages : 151
Book Description
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
Author: Winfried Hartmeier Publisher: Springer Science & Business Media ISBN: 3642733646 Category : Science Languages : en Pages : 217
Book Description
Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.
Author: Bo Mattiasson Publisher: CRC Press ISBN: 1351090283 Category : Science Languages : en Pages : 254
Book Description
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
Author: Ghasem Najafpour Publisher: Elsevier ISBN: 0444633774 Category : Technology & Engineering Languages : en Pages : 669
Book Description
Biochemical Engineering and Biotechnology, 2nd Edition, outlines the principles of biochemical processes and explains their use in the manufacturing of every day products. The author uses a diirect approach that should be very useful for students in following the concepts and practical applications. This book is unique in having many solved problems, case studies, examples and demonstrations of detailed experiments, with simple design equations and required calculations. Covers major concepts of biochemical engineering and biotechnology, including applications in bioprocesses, fermentation technologies, enzymatic processes, and membrane separations, amongst others Accessible to chemical engineering students who need to both learn, and apply, biological knowledge in engineering principals Includes solved problems, examples, and demonstrations of detailed experiments with simple design equations and all required calculations Offers many graphs that present actual experimental data, figures, and tables, along with explanations
Author: R. D. Tyagi Publisher: CRC Press ISBN: 9780849351761 Category : Technology & Engineering Languages : en Pages : 294
Book Description
The purpose of this state-of-the-art publication is to provide up-to-date and pertinent scientific information concerning immobilized cell processes for treatment of wastewater. It comprehensively reviews and examines essential data on the feasibility of various immobilization methods, with special reference to wastewater treatment. It also discusses methods of identification of structure and composition of microbial aggregates and analytical methods for the estimation of biomass in the presence of carriers. This work reports the novel process of immobilized microalgae and cyanobacteria for wastewater treatment, while highlighting their future prospects. Additionally, it critically reviews the various fixed film bioreactor configurations. This book is a must for all engineers, planners, scientists, students and sewage treatment plant operators.
Author: Colin R. Phillips
Author: Colin R. Phillips
Author: Ichiro Chibata
Author: Jose M. Guisan
Author: J. Tampion
Author: Jose M. Guisan
Author: Mattiasson
Author: Winfried Hartmeier
Author: Bo Mattiasson
Author: Ghasem Najafpour
Author: R. D. Tyagi