Imaging Cell Signaling PDF Download

Author: Christoph Wülfing
Publisher: Springer Nature
ISBN: 1071638343
Category :
Languages : en
Pages : 248

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Author: Margarida Barroso
Publisher: CRC Press
ISBN: 1351704494
Category : Science
Languages : en
Pages : 444

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Book Description
This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 50

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Book Description
This highly interdisciplinary team has developed dual-color, total internal reflection microscopy (TIRF-M) methods that enable us to optically detect and track in real time protein migration and clustering at membrane interfaces. By coupling TIRF-M with advanced analysis techniques (image correlation spectroscopy, single particle tracking) we have captured subtle changes in membrane organization that characterize immune responses. We have used this approach to elucidate the initial stages of cell activation in the IgE signaling network of mast cells and the Toll-like receptor (TLR-4) response in macrophages stimulated by bacteria. To help interpret these measurements, we have undertaken a computational modeling effort to connect the protein motion and lipid interactions. This work provides a deeper understanding of the initial stages of cellular response to external agents, including dynamics of interaction of key components in the signaling network at the 'immunological synapse, ' the contact region of the cell and its adversary.

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 17

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Book Description

Author: Eric Hanssen
Publisher: Springer
ISBN: 3319689975
Category : Science
Languages : en
Pages : 339

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Book Description
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Author: Richard N. Day
Publisher:
ISBN: 9781306866255
Category :
Languages : en
Pages : 340

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Book Description
Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a new era of investigations in cell biology, medicine, and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins, The Fluorescent Protein Revolution explores the history, properties, and applications of these important probes. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers several cutting-edge imaging applications. These include superresolution microscopy of cellular fine structures, FRET microscopy to visualize protein interactions and cell-signaling activities inside living cells, photobleaching and photoactivation techniques to visualize protein behaviors, techniques that exploit plant and algal photoreceptors to enable light-regulated control of enzymatic activities, and the noninvasive imaging of tumor host interactions in living animals. In color throughout, this book presents the fundamental principles and latest advances in the field, including the associated development of imaging techniques that exploit fluorescent proteins. It is accessible to a broad audience, from optical imaging experts to novices needing an introduction to the field."

Author: Richard N. Day
Publisher: CRC Press
ISBN: 1439875081
Category : Science
Languages : en
Pages : 350

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Book Description
Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a new era of investigations in cell biology, medicine, and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins, The Fluorescent Protein Revolution explores the history, properties, and applications of these important probes. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers several cutting-edge imaging applications. These include superresolution microscopy of cellular fine structures, FRET microscopy to visualize protein interactions and cell-signaling activities inside living cells, photobleaching and photoactivation techniques to visualize protein behaviors, techniques that exploit plant and algal photoreceptors to enable light-regulated control of enzymatic activities, and the noninvasive imaging of tumor–host interactions in living animals. In color throughout, this book presents the fundamental principles and latest advances in the field, including the associated development of imaging techniques that exploit fluorescent proteins. It is accessible to a broad audience, from optical imaging experts to novices needing an introduction to the field.

Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Category : Medical
Languages : en
Pages : 505

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Book Description
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Author: Michel M.J. Modo
Publisher: CRC Press
ISBN: 1420004093
Category : Medical
Languages : en
Pages : 440

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Book Description
The ability of molecular and cellular imaging to track the survival, migration, and differentiation of cells in vivo as well as monitor particular gene expression in living subjects is rapidly moving from the research laboratory into daily clinical settings. The interdisciplinary nature of the field mandates a constant dialogue among molecular and

Author: Sajal Ray
Publisher: BoD – Books on Demand
ISBN: 1838800654
Category : Science
Languages : en
Pages : 85

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Book Description
Intracellular signal transduction is an important topic of research in cell and molecular biology. It has a wide range of implications in growth, differentiation, development, animal polarity, cellular response monitoring, and many disease processes, including cancer. The functional homeostasis of a cell is assumed to be maintained by an intricate network of signaling that evolved through natural selection. This book includes chapters written in the frontier areas of research on cell signaling. Issues such as information thermodynamics, master regulation of immune tolerance, genetically encoded reporter circuits and their applications, hypoxia, and vitamin K2 mediated signaling processes are addressed and discussed by eminent researchers of the field.