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Author: Marilyn Kaye Publisher: Skylark ISBN: 0804149895 Category : Juvenile Fiction Languages : en Pages : 107
Book Description
Amy's secret is out! Her worst enemy, Jeanine, knows that Amy is a clone. Now Amy fears the worst. She's sure Jeanine will blab the truth to everyone--maybe even try to sell Amy's story to some sleazy tabloid. But Jeanine never gets a chance to make good on any of her threats. An accident leaves her in a coma. Rumors of foul play spread like wildfire. The number one suspect . . . Amy. Sure, Amy wanted to stop Jeanine's big mouth, but maybe she wasn't the only one. . . .
Author: Marilyn Kaye Publisher: Skylark ISBN: 0804149895 Category : Juvenile Fiction Languages : en Pages : 107
Book Description
Amy's secret is out! Her worst enemy, Jeanine, knows that Amy is a clone. Now Amy fears the worst. She's sure Jeanine will blab the truth to everyone--maybe even try to sell Amy's story to some sleazy tabloid. But Jeanine never gets a chance to make good on any of her threats. An accident leaves her in a coma. Rumors of foul play spread like wildfire. The number one suspect . . . Amy. Sure, Amy wanted to stop Jeanine's big mouth, but maybe she wasn't the only one. . . .
Author: Stephen D. Fairbanks Publisher: Nova Publishers ISBN: 9781590338797 Category : Science Languages : en Pages : 252
Book Description
The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Author: Sudhir Paul Publisher: Springer Science & Business Media ISBN: 1592595383 Category : Medical Languages : en Pages : 445
Book Description
This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.
Author: Clare Davy Publisher: Springer Science & Business Media ISBN: 1592599826 Category : Medical Languages : en Pages : 495
Book Description
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author: Publisher: Elsevier ISBN: 0323139019 Category : Technology & Engineering Languages : en Pages : 446
Book Description
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Among the first books to cover principles and methodology of colloidal gold in microscopy Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book Discusses problems and limitations of techniques Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins Preserving and localizing antigenicity; sectioning; high-resolution morphological localization Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation Applications to biomedical studies Colloidal gold in conjunction with video-enhanced microscopy
Author: Ramit Mehr Publisher: Frontiers Media SA ISBN: 2889195015 Category : Immunologic diseases. Allergy Languages : en Pages : 402
Book Description
The rapid development of new methods for immunological data collection – from multicolor flow cytometry, through single-cell imaging, to deep sequencing – presents us now, for the first time, with the ability to analyze and compare large amounts of immunological data in health, aging and disease. The exponential growth of these datasets, however, challenges the theoretical immunology community to develop methods for data organization and analysis. Furthermore, the need to test hypotheses regarding immune function, and generate predictions regarding the outcomes of medical interventions, necessitates the development of mathematical and computational models covering processes on multiple scales, from the genetic and molecular to the cellular and system scales. The last few decades have seen the development of methods for presentation and analysis of clonal repertoires (those of T and B lymphocytes) and phenotypic (surface-marker based) repertoires of all lymphocyte types, and for modeling the intricate network of molecular and cellular interactions within the immune systems. This e-Book, which has first appeared as a ‘Frontiers in Immunology’ research topic, provides a comprehensive, online, open access snapshot of the current state of the art on immune system modeling and analysis.
Author: Marilyn Kaye
Author: Marilyn Kaye
Author: Atmospheric Physics and Chemistry Laboratory (U.S.)
Author: Atmospheric Physics and Chemistry Laboratory (U.S.)
Author: Stephen D. Fairbanks
Author: Environmental Research Laboratories (U.S.)
Author: Sudhir Paul
Author: Clare Davy
Author: 
Author: Andy Dudak
Author: Ramit Mehr