Hyper-hydrophilic Titanium Surfaces Improve Osteoblast Attachment by Enhancing Fibronectin Adsorption at the Interface PDF Download
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Author: Parisi Ludovica Publisher: ISBN: Category : Languages : en Pages :
Book Description
Background: Implants osseointegration is affected by cell responses at the bone-implant interface, which depends on the chemical composition,micro topography and wettability of the surface, which, in turn, influence protein adsorption.Aim: To investigate whether hyperhydrophilicity alters surface selectivity for fibronectin, a protein which plays a pivotal role in cell adhesionand proliferation.Materials and Methods: Acid-etched sandblasted surfaces were treated through a proprietary process in order to obtain hyper-hydrophilic surfaces. Surfacefeatures were characterized by X-Ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), and themeasurement of the contact angle. Protein adsorption was assessed by SDS-PAGE analysis, and the influence of surface infibronectin adsorption was assayed through Western Blot. Cell attachment to the surfaces was then studied. Murine osteoblastsMC3T3-E1 cells were cultured for 24 hours in complete medium. Their morphology and adhesion were studied through theimmuno-staining for cytoskeleton and for focal adhesions and through the use of SEM microscopy coupled to Focused Ion Beam(FIB). FIB analysis allowed to cut cells during SEM observation and to investigate their interactions with the surface. Furthermore, celladhesion was assessed by quantitating the amount of attached cells to the surface at different experimental points through achemiluminescence assay.Results: Our results showed that the experimental treatment induced a gain of titanium hydrophilicity, without inducing any modification inchemical composition or topography. SDS-PAGE revealed a great amount of proteins adsorbed on treated surfaces, while WesternBlot indicated that hydrophilicity increased the amount of fibronectin adsorbed on the surface.Moreover, hyper-hydrophilic surfaces allowed a closer adhesion of cells. Immuno-staining revealed healthy cells on both surfaces,however cells growing on hydrophilic surfaces expressed a higher amount of focal adhesions. Similarly, the adhesion curve showed afaster cell adhesion to hydrophilic titanium already after 30 minutes of culture. SEM-FIB analysis demonstrated that cells on untreatedsurfaces adhered preferentially to the micro texture peaks, while hyper-hydrophilicity promotes the adhesion of cell body to the peaksthroughout the sample. Cells appeared thinner and spread on the entire surface.Conclusions: Taken together, our data show that hyper-hydrophilicity affects protein adsorption. In particular, we observed a more selectiveadsorption of fibronectin, which may be responsible for improved cell adhesion to micro-patterned titanium.
Author: Parisi Ludovica Publisher: ISBN: Category : Languages : en Pages :
Book Description
Background: Implants osseointegration is affected by cell responses at the bone-implant interface, which depends on the chemical composition,micro topography and wettability of the surface, which, in turn, influence protein adsorption.Aim: To investigate whether hyperhydrophilicity alters surface selectivity for fibronectin, a protein which plays a pivotal role in cell adhesionand proliferation.Materials and Methods: Acid-etched sandblasted surfaces were treated through a proprietary process in order to obtain hyper-hydrophilic surfaces. Surfacefeatures were characterized by X-Ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), and themeasurement of the contact angle. Protein adsorption was assessed by SDS-PAGE analysis, and the influence of surface infibronectin adsorption was assayed through Western Blot. Cell attachment to the surfaces was then studied. Murine osteoblastsMC3T3-E1 cells were cultured for 24 hours in complete medium. Their morphology and adhesion were studied through theimmuno-staining for cytoskeleton and for focal adhesions and through the use of SEM microscopy coupled to Focused Ion Beam(FIB). FIB analysis allowed to cut cells during SEM observation and to investigate their interactions with the surface. Furthermore, celladhesion was assessed by quantitating the amount of attached cells to the surface at different experimental points through achemiluminescence assay.Results: Our results showed that the experimental treatment induced a gain of titanium hydrophilicity, without inducing any modification inchemical composition or topography. SDS-PAGE revealed a great amount of proteins adsorbed on treated surfaces, while WesternBlot indicated that hydrophilicity increased the amount of fibronectin adsorbed on the surface.Moreover, hyper-hydrophilic surfaces allowed a closer adhesion of cells. Immuno-staining revealed healthy cells on both surfaces,however cells growing on hydrophilic surfaces expressed a higher amount of focal adhesions. Similarly, the adhesion curve showed afaster cell adhesion to hydrophilic titanium already after 30 minutes of culture. SEM-FIB analysis demonstrated that cells on untreatedsurfaces adhered preferentially to the micro texture peaks, while hyper-hydrophilicity promotes the adhesion of cell body to the peaksthroughout the sample. Cells appeared thinner and spread on the entire surface.Conclusions: Taken together, our data show that hyper-hydrophilicity affects protein adsorption. In particular, we observed a more selectiveadsorption of fibronectin, which may be responsible for improved cell adhesion to micro-patterned titanium.
Author: Jiajun Luo Publisher: ISBN: Category : Languages : en Pages : 224
Book Description
Implant success strongly depends on the proper integration of bone to biomaterial surface. By the selected retrieval cases, inadequate integration of bone screws was a dominated factor caused failure. The surface modification technology that improve osteointegration by inducing TiO2 nanotubes (NT) on Ti-based implants has a potential applications in orthopaedic implants. NT generated by anodization method provide a vertically aligned nanotube structure that enhances the integration between bone tissue and implant surface by improving osteoblasts attachment. Although cells to NT is positive, the mechanical weakness of NT has also been well-documented and is an obstacle to its applications. The thesis comprise a detailed method to improve NT mechanical stability, by introducing an interfacial bonding layer at NT bottom and Ti substrate, and retaining vertically aligned nanotubes. The physicochemical properties of this structure optimized TiO2 nanotubes (SO-NT) was systematically characterized, the SO-NT has been demonstrated with improved biotribological and biocorrosive performance. The uniform hyperfine interfacial bonding layer with nano-sized grains exhibited a strong bonding to NT layer and Ti substrate. It was observed, the layer not only effectively dissipates external impacts and shear stress but also acts as a good corrosion resistance barrier to prevent the Ti substrate from corrosion. The SO-NT modified bone screw has also demonstrated with enhanced fretting corrosion resistance than NT and pristine Ti6Al4V on screws. Since the elongated osteoblasts were observed on NT and SO-NT compared with Ti surface, the nanotubes structure has been shown with promoting of osteoblasts attachment. However, the mechanism of cell nanotubes interactions are largely in controversial. In order to reveal the cell-nanomaterial interactions, nanotopographies including Nanoconvex, Nanoconcave and Nanoflat were generated and characterized to evaluate the cell initial attachment behaviour. Human osteoblasts were observed with spindle shape on Nanoconcave, cells on Nanoflat were well-spreading but in sphere shape, while the osteoblasts on Nanoconvex were with the minimum spreading areas. Cell-materials interface is mediated and influenced by the adsorption of ECM on nanomaterials. Thus, a novel fibronectin adsorption model was proposed by calculating Coulomb's force to illustrate the interact mechanism between protein and material that influence cell behaviours. The achievements of thesis are; 1. Retrieval analyzed two cases of implants failure and pointed out one of dominated failure factor, the lack of osteointegration. 2. Introduce the interfacial bonding layer that significantly improve the biotribological and biocorrosive performance of NT, and generated SO-NT. 3. Systematically evaluated the biotribological performance of Ti, NT and SO-NT, and propose a novel methodology to quantify the fretting degradation on bone screws. 4. Propose a novel model to estimate the fibronectin adsorption on Nanoflat, Nanoconvex and Nanoconcave by the Coulomb's force calculation.
Author: Publisher: John Wiley & Sons ISBN: 3527321551 Category : Technology & Engineering Languages : en Pages : 453
Book Description
The book series Nanomaterials for the Life Sciences, provides an in-depth overview of all nanomaterial types and their uses in the life sciences. Each volume is dedicated to a specific material class and covers fundamentals, synthesis and characterization strategies, structure-property relationships and biomedical applications. The series brings nanomaterials to the Life Scientists and life science to the Materials Scientists so that synergies are seen and developed to the fullest. Written by international experts of various facets of this exciting field of research, the series is aimed at scientists of the following disciplines: biology, chemistry, materials science, physics, bioengineering, and medicine, together with cell biology, biomedical engineering, pharmaceutical chemistry, and toxicology, both in academia and fundamental research as well as in pharmaceutical companies. VOLUME 5 - Nanostructured Thin Films and Surfaces
Author: Mehdi Afshari Publisher: Woodhead Publishing ISBN: 0081009119 Category : Technology & Engineering Languages : en Pages : 650
Book Description
Electrospun Nanofibers covers advances in the electrospinning process including characterization, testing and modeling of electrospun nanofibers, and electrospinning for particular fiber types and applications. Electrospun Nanofibers offers systematic and comprehensive coverage for academic researchers, industry professionals, and postgraduate students working in the field of fiber science. Electrospinning is the most commercially successful process for the production of nanofibers and rising demand is driving research and development in this field. Rapid progress is being made both in terms of the electrospinning process and in the production of nanofibers with superior chemical and physical properties. Electrospinning is becoming more efficient and more specialized in order to produce particular fiber types such as bicomponent and composite fibers, patterned and 3D nanofibers, carbon nanofibers and nanotubes, and nanofibers derived from chitosan. Provides systematic and comprehensive coverage of the manufacture, properties, and applications of nanofibers Covers recent developments in nanofibers materials including electrospinning of bicomponent, chitosan, carbon, and conductive fibers Brings together expertise from academia and industry to provide comprehensive, up-to-date information on nanofiber research and development Offers systematic and comprehensive coverage for academic researchers, industry professionals, and postgraduate students working in the field of fiber science
Author: Johann Reithmaier Publisher: Springer Science & Business Media ISBN: 1402099169 Category : Technology & Engineering Languages : en Pages : 531
Book Description
Nanoscience and Nanotechnology are experiencing a rapid development in many aspects, like real-space atomic-scale imaging, atomic and molecular manipulation, nano-fabrication, etc. , which will have a profound impact not only in every field of research, but also on everyday life in the twenty-first century. The common efforts of researchers from different countries and fields of science can bring complementary expertise to solve the rising problems in order to take advantage of the nanoscale approaches in Materials Science. Nanostructured materials, i. e. materials made with atomic accuracy, show unique properties as a consequence of nanoscale size confinement, predominance of interfacial phenomena and quantum effects. Therefore, by reducing the dimensions of a structure to nanosize, many inconceivable properties will appear and may lead to different novel applications from na- electronics and nanophotonics to nanobiological systems and nanomedicine. All this requires the contribution of multidisciplinary teams of physicists, chemists, materials scientists, engineers and biologists to work together on the synthesis and processing of nanomaterials and nanostructures, und- standing the properties related to the nanoscale, the design of nano-devices as well as of new tools for the characterization of nano-structured materials. The first objective of the NATO ASI on Nanostructured Materials for Advanced Technological Applications was to assess the up-to-date achie- ments and future perspectives of application of novel nanostructured materials, focusing on the relationships material structure ? functional properties ? possible applications.
Author: Hans G. Börner Publisher: Springer Science & Business Media ISBN: 3642201547 Category : Technology & Engineering Languages : en Pages : 232
Book Description
Erik Wischerhoff, Nezha Badi, André Laschewsky and Jean-François Lutz Smart Polymer Surfaces: Concepts and Applications in Biosciences; S. Petersen, M. Gattermayer and M. Biesalski Hold on at the Right Spot: Bioactive Surfaces for the Design of Live-Cell Micropatterns; Julien Polleux Interfacing Cell Surface Receptors to Hybrid Nanopatterned Surfaces: A Molecular Approach for Dissecting the Adhesion Machinery; Abigail Pulsipher and Muhammad N. Yousaf Self-Assembled Monolayers as Dynamic Model Substrates for Cell Biology; D. Volodkin, A. Skirtach and H. Möhwald LbL Films as Reservoirs for Bioactive Molecules; R. Gentsch and H. G. Börner Designing Three-Dimensional Materials at the Interface to Biology; Joerg C. Tiller Antimicrobial Surfaces;
Author: S.V. Bhat Publisher: Springer Science & Business Media ISBN: 9401003289 Category : Science Languages : en Pages : 273
Book Description
As biomaterials are used in medical devices, meeting needs in such diverse surgical disciplines as ophthalmology, cardiology, neuromuscular surgery, orthopaedics, dentistry, etc., they must have intimate contact with patient's tissue or body fluids, providing a real physical interface which seriously restricts developments. This book is written for those who would like to advance their knowledge of biomaterials. The subject matter of the book is divided into twelve chapters dealing with the structure and relationship of biological and man-made biomaterials. The application of these materials for various medical devices, and recent developments in tissue engineering, are also discussed.
Author: Challa S.S.R. Kumar Publisher: Springer Nature ISBN: 3662595966 Category : Technology & Engineering Languages : en Pages : 467
Book Description
Ninth volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Nanotechnology Characterization Tools for Tissue Engineering and Medical Therapy. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.
Author: Savaş Kaya Publisher: CRC Press ISBN: 1000552705 Category : Technology & Engineering Languages : en Pages : 341
Book Description
Materials Development and Processing for Biomedical Applications focuses on various methods of manufacturing, surface modifications, and advancements in biomedical applications. This book examines in detail about five different aspects including, materials properties, development, processing, surface coatings, future perspectives and fabrication of advanced biomedical devices. Fundamental aspects are discussed to better understand the processing of various biomedical materials such as metals, ceramics, polymers, composites, etc. A wide range of surface treatments are covered in this book that will be helpful for the readers to understand the importance of surface treatments and their future perspectives. Additional Features Include: Examines various properties of biomedical materials at the beginning in several chapters which will enrich the fundamental knowledge of the readers. Discusses advancements in various fields of biomedical applications. Provides a glimpse of characterization techniques for the evaluation of material properties. Addresses biocompatibility, biocorrosion, and tribocorrosion. This book explores new and novel strategies for the development of materials and their biomedical applications. It will serve as a comprehensive resource for both students and scientists working in materials and biomedical sciences.
Author: G. Björn Stark Publisher: ISBN: Category : Medical Languages : en Pages : 330
Book Description
C.A. VACANTI The loss or failure of an organ or tissue is one of the most frequent, devastating, and costly problems in human health care. Advances in the medical sciences have enabled physicians to restore lost functions in their patients through or gan transplantation, reconstructive surgery with autogenous tissue transfer, or the implantation of alloplastic materials. Although only recently termed "Tissue Engineering" [1], the use of biological andlor synthetic materials in conjunction with cells to create biologic substitutes to serve as functional tis sue replacements has been explored by modern scientists for several decades. The concept of generating functional tissue from an organ biopsy was de scribed very early in the literature: "The Lord God cast a deep sleep on the man, and while he was asleep, he took out one of his ribs and closed up its place with flesh. The Lord God then built up into a woman the rib that he had taken from the man" [2]. Review of history further reveals that, according to legend, the first homotransplantation of an entire limb was performed by Saints Damian and Cosmas, as depicted by the artist Fra Angelico.
Author: Parisi Ludovica
Author: Parisi Ludovica
Author: Jiajun Luo
Author: 
Author: Mehdi Afshari
Author: Johann Reithmaier
Author: Hans G. Börner
Author: S.V. Bhat
Author: Challa S.S.R. Kumar
Author: Savaş Kaya
Author: G. Björn Stark