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Author: Marlene Belfort Publisher: Springer Science & Business Media ISBN: 9783540852353 Category : Science Languages : en Pages : 404
Book Description
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Author: Marlene Belfort Publisher: Springer Science & Business Media ISBN: 9783540852353 Category : Science Languages : en Pages : 404
Book Description
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Author: Gregory K. Taylor Publisher: ISBN: Category : Languages : en Pages : 97
Book Description
Homing endonucleases, found in all forms of microbial life, facilitate the invasion of host genes often in concert with introns or inteins by generating double stranded breaks in conserved coding sequences. There are five homing endonuclease families distinct in their structural characteristics and each family appears to share a common ancestor with diverse host proteins of unrelated function. Such related proteins include restriction endonucleases, DNA mismatch repair proteins, transcription factors, four-way junction resolving enzymes, and colicins. Homing endonculeases are currently being computationally redesigned for applications in genome engineering and structures of three redesigned homing endonuclease variants are described. In these experiments, crystal structures uncovered unexpected shifts in the DNA backbone relative to the wild type endonucleases and have thus been informative in the redesign process. Recently, a sixth homing endonuclease family homologous to E. Coli DNA repair protein VSR was discovered. A series of biochemical and x-ray crystallographic experiments investigating binding specificity and catalytic mechanism of a representative family member I-Bth0305I are described. Finally, a database archiving experimentally characterized homing endonucleases and a web-base program supporting homing endonuclease target site search are discussed.
Author: Roger Bertolotti Publisher: World Scientific ISBN: 9812775862 Category : Medical Languages : en Pages : 489
Book Description
Stem cells provide for life-long cell replacement in tissues and organs, and have inherent homing abilities that are critical in therapeutic applications. Stem cells are also the driving force of cancer where genetic/epigenetic alterations culminate in tumorigenesis either in tissue stem cells or in some of their derivatives. As a rare subset of the tumor, cancer stem cells are the only drive of tumor initiation/propagation. Autologous and cancer stem cells are thus the key targets of 1) long-term and transient-regenerative/epigenetic gene therapy and 2) of recurrence-free anticancer therapy, respectively. While cancer stem cell gene therapy still needs time to accomplish, autologous stem cells have been instrumental in the first unequivocal successes for gene therapy whereby ex vivo retrovirally corrected hematopoietic stem cells have been returned to the patients.This timely book presents 1) the aforementioned stem cell gene therapy achievements that rely on random-integration of therapeutic transgenes into host chromosomes and 2) emerging experimental approaches aimed at eliminating random-integration oncogenic hazards through site-specific integration or gene targeting. Breakthrough endonuclease-boosted gene targeting for gene correction (inherited diseases) or targeted integration of therapeutic transgenes (other disorders) culminating in an efficiency compatible with clinical trials is one of the highlights of the book. Highlights also include the pioneering transplantation of adult pluripotent stem cells as a substitute for tissue-specific stem cells, thereby pinpointing the invaluable potential for stem cell gene therapy applications of autologous cells able to contribute to all three germ layers. Stem cell gene therapy is thus discussed in terms of 1) magnifying stem cell therapeutic homing through transient regenerative gene therapy and 2) of tackling most pathologies (including mitochondrial DNA diseases and ageing disorders) through stem cell repopulation dynamics into appropriate niches (long-term engraftment) and tissues (cell turn-over). Regarding cancer stem cell gene therapy, focus is on both the increasing number of identified tissue-specific cancer stem cells as the ultimate therapeutic targets and on the development of armed stem cells as tumor-homing vectors for targeted anticancer therapy.
Author: Francesca Storici Publisher: Humana ISBN: 9781493963218 Category : Medical Languages : en Pages : 0
Book Description
Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.
Author: Colin Kleanthous Publisher: Frontiers in Molecular Biology ISBN: 9780199637607 Category : Carrier proteins Languages : en Pages : 370
Book Description
The purpose of Protein-Protein Recognition is to bring together concepts and systems pertaining to protein-protein interactions in a single unifying volume. In the light of the information from the genome sequencing projects and the increase in structural information it is an opportune time totry to make generalizations about how and why proteins form complexes with each other. The emphasis of the book is on heteromeric complexes (complexes in which each of the components can exist in an unbound state) and will use well-studied model systems to explain the processes of formingcomplexes. After an introductory section on the kinetics, thermodynamics, analysis, and classification of protein-protein interactions, weak, intermediate, and high affinity complexes are dealt with in turn. Weak affinity complexes are represented by electron transfer proteins and integrincomplexes. Anti-lysozyme antibodies, the MHC proteins and their interactions with T-cell receptors, and the protein interactions of eukaryotic signal transduction are the systems used to explain complexes with intermediate affinities. Finally, tight binding complexes are represented by theinteraction of protein inhibitors with serine proteases and by nuclease inhibitor complexes. Throughout the chapters common themes are the technologies which have had the greatest impact, how specificity is determined, how complexes are stabilized, and medical and industrial applications.
Author: Boriana Marintcheva Publisher: Academic Press ISBN: 0128105151 Category : Science Languages : en Pages : 298
Book Description
Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in vitro and in vivo; ii) Protein expression and characterization; and iii) Virus- Host interactions as a platform for therapy and biocontrol are discussed. It steers away from traditional views of viruses and technology, focusing instead on viral molecules and molecular processes that enable science to better understand life and offer means for addressing complex biological phenomena that positively influence everyday life. The book is written at an intermediate level and is accessible to novices who are willing to acquire a basic level of understanding of key principles in molecular biology, but is also ideal for advanced readers interested in expanding their biological knowledge to include practical applications of molecular tools derived from viruses. - Explores virus-based tools and approaches in DNA manipulation, protein expression and characterization and virus-host interactions - Provides a dedicated focus on viral molecules and molecular processes that enable science to better understand life and address complex biological phenomena - Includes an overview of modern technologies in biology that were developed using viral components/elements and knowledge about viral processes
Author: Carl A. Pinkert Publisher: Newnes ISBN: 0124095364 Category : Science Languages : en Pages : 715
Book Description
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. - Contains a comprehensive collection of transgenic animal and gene transfer methods - Discusses background and introduction to techniques and animal systems - Teaches practical step-by-step protocols - Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Author: Marlene Belfort
Author: Marlene Belfort
Author: Alireza Ghodsi Senejani
Author: Gregory K. Taylor
Author: Roger Bertolotti
Author: Annika Anna Maria Bokor
Author: Francesca Storici
Author: Colin Kleanthous
Author: Boriana Marintcheva
Author: Christopher K. Mathews
Author: Carl A. Pinkert