Author: D. Metcalf Publisher: Springer Science & Business Media ISBN: 364281140X Category : Medical Languages : en Pages : 397
Book Description
The cloning of hemopoietic cells in semisolid medium began some 12 years ago, and when colonies of T = and B = lymphocytes were grown by several groups last year, the last major subclasses of hemopoietic cells had been successfully cultured in vitro. The time seemed appropriate to write a short general account of this exciting new area of hematology, emphasizing particularly the potential value of these new techniques for direct studies on patients with leukemia and allied diseases. I had hoped to refer to, or at least record, every paper published in this field, but the literature proved to be larger than I had realized, and furthermore, some areas were so active that chapters had to be rewritten over a period of less than a year. I regret that ·what follows is an incomplete account and no doubt, by the time this book becomes available, the material will be even more out of date. However, I have described the basic techniques in some detail and have tried, where possible, to explain how and why the techniques can fail. It is already possible to see patterns, perhaps even principles, emerging from this work which may well be applicable to other cell populations. These have been emphasized in the text in an attempt to make this review as creative as possible.
Author: Publisher: Karger Medical and Scientific Publishers ISBN: 3805578849 Category : Medical Languages : en Pages : 97
Book Description
Practical and concise, this guide addresses clinical and experimental hematologists, technicians and teachers interested in human hematopoietic stem cells. Offering a unique collection of photographs taken at steady reproducible culture conditions by a hematologist with extensive clinical and experimental experience, it fills a gap in the current hematology literature.
Author: Leonard I. Zon Publisher: Oxford University Press ISBN: 9780199771752 Category : Medical Languages : en Pages : 864
Book Description
Hematopoiesis, or the process of blood formation, has been extensively studied at both basic and clinical levels. Human diseases such as thalassemia, immunodeficiency, and leukemia represent defects in this process. Approaches to treat these disorders have required a basic understanding of the biology of blood cells. For instance, hemapoietic stem cell replacement or bone marrow transplantation has been used to ameliorate disease. This volume focuses on hematopoiesis at a cellular and molecular level, and establishes the basis for clinical manipulation of hematopoietic cells for therapeutic benefit. In Part I, the cellular characteristics of progenitors and stem cells are explored. Emphasis is placed on purification of stem cells and both in vitro and in vivo assays. The regulation of normal and leukemis stem cells is illustrated. An excellent discussion of potential use of these cells for gene therapy concludes this section. Hemapoiesis is easily studied during embryogenesis. Part II develops the concept of the waves of hemapoiesis during development. Comparative hematology is making a major comeback as a field in the 1990's. One hope is that general principles of hematopoiesis will be established by studying many models and systems. Part III delves into critical factors that regulate hematopoiesis, including both intracellular and extracellular signals. Part IV and V describe lineage programs for myeloid and lymphoid lineages. These chapters are meant to be illustrative of the different cell fates, but are not exhaustive. Part VI examines the genetics of hematopoisis, particularly in animal models. The hematopoietic system is in constant contact with stromal cells and endothelial cells during development and in the adult. Evidence suggests that endothelial cells and blood cells may arise from a common progenitor, the hemangioblast. Part VII and VIII discuss the stromal and endothelial cells with the emphasis on their interaction with hematopoietic cells.
Author: Donald Metcalf Publisher: Harvard University Press ISBN: 9780674581579 Category : Blood cells Languages : en Pages : 182
Book Description
This book presents a masterful overview of the mechanics of blood cell formation and the factors that control blood cell growth. Cells circulating in the blood perform functions essential for the survival of organisms, yet blood cells and associated blood-cell-forming (hemopoietic) tissues have certain features that make them quite different from other vital organs in the body. These features include the short life span of mature blood cells, the multiplicity of blood cell types, and the wide dispersion of hemopoietic tissue in the body. The regulation of hemopoiesis in response to emergencies such as blood loss or infections is an exceedingly complex process. However, our knowledge of hemopoietic growth factors, known generally as colony-stimulating factors or CSFs, has made exciting advances in recent years. This book provides a leading authority's review of the purification and cloning of CSFs and their actions in regulating white blood cell production in the body. Donald Metcalf also examines the role of CSFs in controlling resistance to infections and in the initiation and suppression of myeloid leukemia. Metcalf's involvement at the center of this research from its inception to the present day enables him to give a historical view as well as a lucid summary of recent research. In addition, he addresses the broader question of the relation between growth control and cancer development. This excellent synopsis should be of particular interest to hematologists, virologists, general clinicians, and medical students.
Author: D. Metcalf
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Author: William A. Robinson
Author: Donald Metcalf
Author: Leonard I. Zon
Author: Donald Metcalf
Author: Bayard Clarkson
Author: National Cancer Institute (U.S.)
Author: M. Bessis