Genomics of Antibiotic Resistant Bacteria in Industrial Waste Water Treatment PDF Download

Author: Maulin P. Shah
Publisher: Springer Nature
ISBN: 3031446186
Category : Science
Languages : en
Pages : 129

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Book Description
This book discusses the key problems and solutions with various applicable approaches to combat antibiotic-resistant genes in industrial waste water. Several genes are selected within the chapters to illustrate the past and future roles of molecular ecophysiology and genomics in the development of wastewater microbiology as an important subdiscipline of microbial ecology. As we have very limited knowledge of composition, dynamics and stability of microbial communities, various processes in wastewater treatment have been generally considered to be "black box." In recent years, with the development of several new high throughput sequencing platforms, metagenome sequencing strategies and bioinformatics toolboxes, the analysis of the genome of complex communities has become much more accessible and means easier. The opening of the biological wastewater treatment “black box” is not the unpleasant experience it was before. The viable, but not cultural, ceases to be the inconsequential, uncharacterizable enigma that existed today. Metagenomics leads the way for more specific studies in related fields. Finally, genomic studies of wastewater treatment microbes, in addition to their biotechnological applications, are also an excellent testing ground for variety of other ecological and environmental burns questions. Wastewater treatment plants are considered hotspots for the environmental dissemination of antimicrobial-resistant determinants. Comparative genomics of antibiotic resistant genes isolated from conventional activated sludge and biological aerated filter wastewater treatment plants is discussed.

Author: Claire Nicole Freeman
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Book Description
Wastewater produced from human activity contains high concentrations of contaminants like ammonia, pathogens, and organic compounds with high biological oxygen demand; all of which must be remediated with proper wastewater treatment prior to discharge into the environment. Most large-scale wastewater treatment plants (WWTPs) remove contaminants from wastewater using the activated sludge process, wherein wastewater is mixed with a flocculent slurry of microorganisms that use these contaminants for the production of energy or increasing microbial biomass. The activated sludge environment is a complex microbial ecosystem, containing stable populations of organisms adapted to activated sludge, as well as transient environmental and human gutassociated organisms. These microbial communities are subject to various selective pressures, including predation, fluctuating temperatures, sub-therapeutic levels of antibiotics, and heavy metals. Although microbial activity plays a large role in the functionality of these systems, there is limited information about the resident microbial communities within. In general, the bacteria in these systems are monitored through microscopy, staining, and culturing. While these tools are useful, they provide limited information about the microbial communities as a whole. Thus, this work aims to use higher resolution tools like high-throughput metagenomic sequencing and quantitative PCR to gain more information about the microbial communities in a wastewater treatment environment. This thesis focuses on three aspects of the microbial communities in the Regina WWTP: (1) the composition of the activated sludge microbial community and how it changes in response to seasonality (2) mobile genetic elements in untreated wastewater and activated sludge, and (3) the antimicrobial resistance (AMR) genes that are released into Wascana creek in the treated wastewater effluent. Samples of WWTP effluent, untreated wastewater, and activated sludge were collected from the Regina WWTP. DNA was extracted from all samples and used in high throughput sequencing to determine information about microbial community composition and mobile genetic elements, or quantitative PCR to quantify the AMR genes leaving the WWTP in the effluent. The results indicated that the Regina WWTP activated sludge system is primarily composed of the bacterial phyla, Proteobacteria and Actinobacteria. Although the composition of this microbial community varied seasonally at the genus level, the performance of the WWTP remained stable, suggesting that the functionality of this system is robust to some change within the microbial community. Quantification of functional genes revealed that denitrification genes were abundant, while nitrification genes were not, likely due to nitrifying organisms accounting for a very low proportion of the overall microbial biomass. In the recovered collection of mobile genetic elements, plasmids providing resistance to metals and antibiotics were abundant, which likely contribute to microbial adaptation to these environments. Additionally, quantification of antibiotic resistance genes in the treated effluent revealed that some of these genes are surviving the wastewater treatment process, suggesting that WWTPs may be contributing to the dissemination of antibiotic resistance in the environment.

Author: Maulin P. Shah
Publisher: Elsevier
ISBN: 0128239913
Category : Science
Languages : en
Pages : 644

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Book Description
Wastewater Treatment Reactors: Microbial Community Structure analyzes microbial community structure in relation to changes in physico-chemical parameters, the gene content (metagenome) or gene expression (metatranscriptome) of microbial communities in relation to changes in physico-chemical parameters, physiological aspects of microbial communities, enrichment cultures or pure cultures of key species in relation to changes in physico-chemical parameters, and modeling of potential consequences of changes in microbial community structure or function for higher trophic levels in a given habitat. As several studies have been carried out to understand bulking phenomena and the importance of environmental factors on sludge settling characteristics, which are thought to be strongly influenced by flocculation, sludge bulking, foaming and rising, this book is an ideal resource on the topics covered. Presents the state-of-the-art techniques and applications of omics tools in wastewater treatment reactors (WWTRs) Describes both theoretical and practical knowledge surrounding the fundamental roles of microorganisms in WWTRs Points out the reuse of treated wastewater through emerging technologies Covers the economics of wastewater treatment and the development of suitable alternatives in terms of performance and cost effectiveness Discusses cutting-edge molecular biological tools Gives in-depth knowledge to study microbial community structure and function in wastewater treatment reactors

Author: Damia Barcelo
Publisher: Springer
ISBN: 364228132X
Category : Science
Languages : en
Pages : 475

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Book Description
This volume provides an overview of the occurrence and fate of emerging contaminants, discusses advanced chemical analysis methods, toxicological and ecotoxicological effects as well as human exposure. One focus is on pharmaceuticals, in particular antibiotics, and the problems associated with their increased use in hospitals. Other covered emerging contaminants occurring e.g. in food, water, air or soil include brominated flame retardants, polar pesticides, phthalates, phosphate esters, perfluorinated compounds, personal care products, musk fragrances, disinfection byproducts, illicit drugs, and nanomaterials. The chapters written by experts are a valuable source of information for a broad audience, such as analytical chemists, environmental chemists and engineers, toxicologists, ecotoxicologists and epidemiologists working already in this field as well as newcomers.

Author: S. Bathe
Publisher: IWA Publishing
ISBN: 9781843395096
Category : Science
Languages : en
Pages : 186

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Book Description
Aerobic Granular Sludge has recently received growing attention by researchers and technology developers, worldwide. Laboratory studies and preliminary field tests led to the conclusion that granular activated sludge can be readily established and profitably used in activated sludge plants, provided 'correct' process conditions are chosen. But what makes process conditions 'correct'? And what makes granules different from activated sludge flocs? Answers to these question are offered in Aerobic Granular Sludge. Major topics covered in this book include: Reasons and mechanism of aerobic granule formation Structure of the microbial population of aerobic granules Role, composition and physical properties of EPS Diffuse limitation and microbial activity within granules Physio-chemical characteristics Operation and application of granule reactors Scale-up aspects of granular sludge reactors, and case studies Aerobic Granular Sludge provides up-to-date information about a rapidly emerging new technology of biological treatment.

Author: Dabojani Das
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Book Description
The overuse and misuse of antibiotics to treat bacterial infections, the release of unmetabolized residuals into the sewer system, and the incomplete removal antibiotic residues by wastewater treatment plants (WWTPs) pose a severe threat to human health. The accumulation of antibiotic residue induces selective pressure on the bacterial population, resulting in the spread of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) in water. This study investigated the degradation of different types of ARGs in water matrices using a wide variety of treatment technologies. Real wastewater samples were collected from a WWTP in urban Philadelphia and the presence of single and multidrug-resistant bacteria and resistance genes were investigated using molecular-based techniques. Subsequently, an analytical method was developed and validated for the detection and quantification of the ARGs against a range of antibiotics, such as tetracycline (TCN), ciprofloxacin (CIP), and levofloxacin (LVX). Finally, to remove the ARGs from water matrices, different conventional and advanced oxidation processes were applied. At the very onset, conventional treatment processes such as chlorine treatment was used to inactivate the E.coli resistant strains. It was observed that chlorination can potentially deactivate the ARBs by applying a lower dose and contact time. However, the effectiveness of chlorine treatment in removing all types of ARGs from water matrices was limited. For instance, no significant degradation of extracellular ARGs (e-ARGs) was observed in DI water during chlorine treatment. Subsequently, a peracetic acid (PAA) based treatment process was used to degrade the genomic and plasmid-encoded ARGs from the water matrices. Similar to chlorine treatment, no significant changes were observed in the degradation of extracellular ARGs (e-ARGs) in DI and WW. Then, the degradation kinetics of ARGs across different types (gyrAR, tetAR, qnrSR) and forms (chromosomal, plasmids) were evaluated using the Ultraviolet (UV) disinfection process. Compared to chlorination and PAA, UV treatment showed better removal efficiencies for the degradation of different types of e-ARGs in DI water. The degradation profile of e-ARGs showed 1-4 log reductions at a UV fluence of 900 mj/cm2. The i-ARGs showed similar degradation rates as compared to e-ARGs in phosphate buffer saline (PBS) at the same UV dosage. On the other hand, the regrowth potential of ARBs at low UV dosage (60-180 mJ/cm2) showed the evidence of damage repairment after several hours of exposure to light (photoreactivation) and dark conditions, making it susceptible again to the resistance spread. To resolve this issue, process parameters were optimized, and no regrowth of the ARBs were found from the higher fluence from 300 to 600 mJ/ cm2. Later, UV/ H2O2 based AOP was applied to evaluate the degradation and deactivation of the same resistant genes. The addition of H2O2 during the UV treatment produces strongly reactive •OH radicals during the treatment and showed considerable improvements in e-ARGs degradation (1.2-5 logs) compared to UV treatment alone. However, this AOP showed minimal contribution to i-ARG degradation (1-2.4 logs), possibly due to the scavenging of •OH radicals by the cellular components in PBS. In contrast to PBS, the wastewater matrix moderately enhanced the gene degradation during the treatment. In terms of plasmid degradation, the conformational differences of the supercoiled structures showed 1.2-2.8 times slower degradation rates than chromosomal ARGs. In addition, the degradation kinetics of the free residual ARGs (f-ARGs) were assessed during the treatment to reduce the AMR dissemination risk from the treated sample. This study also examined the potential of ozone (O3) based oxidation process to degrade and deactivate the extracellular and intracellular ARGs, and MGE (plasmid, intl-1) from E.coli ARBs. The degradation kinetics of the ARGs across different sizes (118-454 bps) and types were evaluated in different water matrices (DI water, PBS, and WW), and showed a significantly higher removal for chromosomal, and plasmid encoded ARGs than other treatment technologies. For the e-ARGs in DI water, 3.8-5.2 logs removal was observed at ozone dosage of 2.0 × 10-2 M.s. i-ARGs in PBS and wastewater showed nearly similar degradation (3.8-5 logs) during O3, indicating the elimination of i-ARGs was not dependent on the cellular components and effluent organic matter. Moreover, an analysis of environmental DNA (eDNA) from wastewater was conducted to examine the degradation of DNA and ARGs for different storage periods and temperatures (-20°C, 0°C, 4°C, 22±0.87°C). Result indicated that water samples kept at -20°C and 0°C showed the best performance in preventing the DNA concentration and gene degradation over time. Additionally, the effectiveness of different preservatives (Longmire buffers: LB1 and LB2, benzalkonium chloride at 0.1%, 0.01%) were investigated in preserving the DNA integrity and the gene degradation at an ambient temperature. It was found that the Longmire buffer (LB1) exhibited lowest gene degradation during the three-week storage period. In summary, this research provided a comprehensive assessment on the degradation of e-ARGs, i-ARGs, and free ARGs from water using different treatment technologies (i.e., UV, UV/H2O2, O3, PAA, chlorine). Additionally, this study suggested valuable information on optimizing the process parameters of the selected methods and developed a comparative assessment of removing the ARGs from the water matrix (DI/PBS, WW). The estimation of Electrical Energy per Order (EEO, kWh/m3) during UV and ozone treatments provided a comparison of the energy consumption for ARGs degradation in the water. Overall, the findings of this study can be useful for evaluating different types and forms (chromosomal, plasmid) of ARG degradation from water matrices and can help to reduce the risk of AMR dissemination in the environment.

Author: Muhammad Zaffar Hashmi
Publisher: Elsevier
ISBN: 0128188839
Category : Science
Languages : en
Pages : 434

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Book Description
Antibiotics and Antimicrobial Resistance Genes (AMR) in the Environment summarizes and updates information on antibiotic producing organisms and their resistance and entry routes in soil, air, water and sediment. As antibiotic use continues to rise in healthcare, their fate, bioavailability and biomonitoring, and impacts on environment and public health are becoming increasingly important. The book addresses the impact of antibiotics and AMR to environment and public health and risk assessment. Moreover, it focused on the metagenomics and molecular techniques for the detection of antibiotics and antimicrobial genes. Lastly, it introduces management strategies, such as treatment technologies for managing antibiotics and AMR/ARGs-impacted environment, and bioremediation approaches. Summarizes and updates information on antibiotics and AMR/ARGs production and its fate and transport in the environment Includes phytoremediation and bioremediation technologies for environmental management Provides analysis of risk assessment of antibiotic resistance genes to help understand the environmental and socioeconomic impacts of antibiotics and AMR/ARGs

Author: Maulin P. Shah
Publisher: CRC Press
ISBN: 1000815072
Category : Technology & Engineering
Languages : en
Pages : 253

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Book Description
Focusing on microbial community structure in the field of wastewater treatment, this book highlights structural analyses in relation to changes in physico-chemical parameters. It further covers physiological analyses of microbial communities, enrichment of pure cultures of key species in relation to changes in physico-chemical parameters, and analyses and modelling of consequences of changes in microbial community structure. Based on 16S rRNA gene sequencing, groups of bacteria that perform nitrogen fixation, nitrification, ammonification and other biochemical processes are covered for an entire wastewater treatment plant bioreactor along with temporal dynamics of bacterial communities. Features: Describes the state-of-the-art techniques and the application of omics tools in wastewater treatment reactors (WWTRs). Includes both the theoretical and practical knowledge on the fundamental roles of microorganisms in WWTRs. Discusses environmental microbial community proteomics. Covers relating function and community structure of complex microbial systems using neural networks. Reviews the economics of wastewater treatment and the development of suitable alternatives in terms of performance and cost-effectiveness. This book is aimed at graduates and researchers in biological engineering, biochemical engineering, chemistry, environmental engineering, environmental microbiology, systems ecology and environmental biotechnology.

Author: Negin Malekian
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Author: Matthew A. Tarr
Publisher: CRC Press
ISBN: 9780203912553
Category : Science
Languages : en
Pages : 506

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Book Description
Chemical Degradation Methods for Wastes and Pollutants focuses on established and emerging chemical procedures for the management of pollutants in industrial wastewater and the environment. This reference offers an in-depth explanation of the degradation process, mechanisms, and control factors affecting each method, as well as issues crucial to th