Genetic Manipulation of the Early Mammalian Embryo PDF Download

Author: Frank Costantini
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 312

View

Book Description

Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309676681
Category : Medical
Languages : en
Pages : 155

View

Book Description
Because of the recent advances in embryo modeling techniques, and at the request of the Office of Science Policy in the Office of the Director at the National Institutes of Health, the National Academies of Sciences, Engineering, hosted a 1-day public workshop that would explore the state of the science of mammalian embryo model systems. The workshop, which took place on January 17, 2020, featured a combination of presentations, panels, and general discussions, during which panelists and participants offered a broad range of perspectives. Participants considered whether embryo model systems - especially those that use nonhuman primate cells - can be used to predict the function of systems made with human cells. Presentations provided an overview of the current state of the science of in vitro development of human trophoblast. This publication summarizes the presentation and discussion of the workshop.

Author: Ralph B.L. Gwatkin
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 416

View

Book Description
V.4 - Manipulation of mammalian development.

Author: Richard Behringer
Publisher:
ISBN: 9781936113019
Category : MEDICAL
Languages : en
Pages : 0

View

Book Description
Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.

Author: Roger A. Pedersen
Publisher: Banbury Report
ISBN:
Category : Medical
Languages : en
Pages : 360

View

Book Description
Recent progress in cell and molecular biology has profound implications for understanding the developmental biology of mammalian embryos. The unifying theme of this volume is a cellular and molecular approach to gametogenesis, embryogenesis, and maternal-fetal interactions in the developmental biology of agriculturally significant mammals. Great progress has been achieved in our understanding of preimplantation mammalian embryogenesis in laboratory species, particularly the mouse, through application of cell and embryo culture methods, cell biological techniques, and, more recently, recombinant DNA technology. A major remaining opportunity is the application of these techniques to problems in domestic species, such as improved methods for in vitro growth and maturation of oocytes, egg and embryo cryopreservation, and genetic manipulation to enhance productivity and herd quality. The authors review current knowledge in key areas, emphasizing new approaches to unsolved problems in the developmental biology of domestic species.

Author: Patrick P. L. Tam
Publisher:
ISBN: 9781936113248
Category : Science
Languages : en
Pages : 0

View

Book Description
"A subject collection from Cold Spring Harbor perspectives in biology."

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254

View

Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Author: Brigid Hogan
Publisher:
ISBN: 9780879693848
Category : Embryology
Languages : en
Pages : 0

View

Book Description
A comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sect

Author: Italian Society of Embryo Transfer. Congress
Publisher: California Princeton Fulfillment
ISBN: 9781855780330
Category : Technology & Engineering
Languages : en
Pages : 282

View

Book Description
Gamete maturation. Towards a molecular understanding of meiosis in domestic animals. Biology of oocyte maturation. Post-meiotic sperm development in mammals. Molecular probes for testing bovine sperm quality. Fertilization and embryonic development. Fertilization by assisted micromanipulation of gametes. In vitro fertilization in farm animals. Culture of embryos of farm animals. Maternal control of early embryonic development. Inter - and extraspecies pregnancies in equids. Embryonic manipulation and genetic engineering. Gender preselection in mammals. Embryo multiplication in livestock. Present procedures and the potential for improvement. Gene transfer in farm animals. Mechanism of sperm - DNA interaction. Spermatozoa as vectors for introducing exogenous DNA into eggs. Embryonic stem cells from domestic animals. Establishment and potential applications. New trends in embryo transfer. Embryo transfer and new technology in sheep reproduction. Embryo production in vivo: problems and prospects. Embryotechnology in dairy cattle breeding. Practical application of two advanced biotechnologies to bovine embryo transfer: splitting and sexing. Theoretical and practical aspects of equine embryo transfer. Embryo technology in pets and endangered species. Reproductive biotechnology in deer farming. Vitrification of oocytes and embryos. International regulations. Tentative prospective and health regulations of the embryo transfer industry.

Author: David K. Gardner
Publisher: Oxford University Press
ISBN: 9780195142266
Category : Medical
Languages : en
Pages : 434

View

Book Description
Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.