Genetic Induction of Cultured Human Cells by Femtosecond Pulse Laser-Light Exposure PDF Download
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Author: Publisher: ISBN: Category : Languages : en Pages : 16
Book Description
The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed during operations. The increased potential for exposure of humans to laser light highlights the need for scientifically-based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. We assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) assay, it appears that 910 and 455 nm, femtosecond pulses of laser light is sensed and induces several stress response genes. Both wavelengths induced the xenobiotic receptor element in a roughly dose dependent fashion at 48 hours post exposure; while also inducing other genes to yield a genetic expression fingerprint unique to the exposure parameters. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.
Author: Publisher: ISBN: Category : Languages : en Pages : 16
Book Description
The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed during operations. The increased potential for exposure of humans to laser light highlights the need for scientifically-based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. We assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) assay, it appears that 910 and 455 nm, femtosecond pulses of laser light is sensed and induces several stress response genes. Both wavelengths induced the xenobiotic receptor element in a roughly dose dependent fashion at 48 hours post exposure; while also inducing other genes to yield a genetic expression fingerprint unique to the exposure parameters. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.
Author: John Obringer Publisher: ISBN: Category : Languages : en Pages : 14
Book Description
The use of laser light for targeting devices and weapons has sharply increased the likelihood that aircrew and support personnel will be exposed to laser light during operations. The increased potential for exposure of humans highlights the fact that there is a need for scientifically based safety standards for laser exposure at the ultrashort pulse lengths. Current safety standards are largely extrapolations of exposure limits at longer pulse lengths using a minimal visible lesion endpoint in the Rhesus monkey retinal model. A non-animal model for assessing laser-light damage to tissue, particularly human, is quite desirous for obvious scientific, political, and fiduciary reasons. I assessed the sublethal insult to human cells using a tissue culture system for specific genes that have been shown to be important in several biological processes that could lead to cancer or cell death. Using the CAT-Tox (L) (xenometrix, Inc.) assay, it appears that 532 nm nanosecond pulse of laser light are sensed and induces several stress response genes including FOS in a roughly dose dependent fashion. This approach provides insight into a more global methodology for characterizing environmental stressors via genetic profiling.
Author: Markolf H. Niemz Publisher: Springer Science & Business Media ISBN: 3662047179 Category : Science Languages : en Pages : 310
Book Description
Basic concepts such as the optical and thermal properties of tissue, the various types of tissue ablation, and optical breakdown and its related effects are treated in detail. Special attention is given to mathematical tools (Monte Carlo simulations, the Kubelka—Munk theory etc.) and approved techniques (photodynamic therapy, laser-induced interstitial thermotherapy etc.). The part on applications reviews clinically relevant methods in modern medicine using the latest references. The last chapter covers today’s standards of laser safety, with a careful selection of essential guidelines published by the Laser Institute of America. With numerous research photographs, illustrations, tables and comprehensive summaries.
Author: Fan-Gang Tseng Publisher: Springer ISBN: 3662491184 Category : Technology & Engineering Languages : en Pages : 415
Book Description
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Author: Publisher: Academic Press ISBN: 9780128123737 Category : Medical Languages : en Pages : 0
Book Description
Role of Nutraceuticals in Chemoresistance to Cancer, Volume Two, focuses on nutraceuticals, the compounds derived from natural sources, which are usually multi-targeted as a means to overcome chemoresistance. This book discusses the role of several compounds related to nutraceuticals and chemoresistance, such as curcumin, resveratrol, indole 3-carbinol, tocotrienols, ursolic acid, fisetin, celastrol, gambogic, butein, catechins and silymarin. It is a valuable resource for cancer researchers, oncologists and members of several areas of the biomedical field who are interested in understanding how to use nutraceuticals as a sensitizing agent for chemotherapy.
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Author: John Obringer
Author: Robert M. Stark
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Author: Jun Zhang
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Author: Markolf H. Niemz
Author: Fan-Gang Tseng
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