Genetic Alteration of Metabolism and Tumorigenicity of Prostate Cancer Cells PDF Download

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 57

View

Book Description
In the prostate, overwhelming evidence now exists that zinc and citrate metabolism are important factors in the pathogenesis and progression of prostate cancer (Pca). This proposal is aiming to establish that the pro static tumor cells obtained from PC-3 and LNCaP-derived tumors are citrate oxidizing cells; and to demonstrate that the genetic alteration of zinc accumulation and expression of m-aconitase will alter citrate oxidation and will correspondingly alter the tumorigenic capabilities of LNCaP and PC-3 cells. The second year study was focused on: 1) to study the zinc effect on the prostate tumorigenicity in vivo; 2) to establish and characterize ZIP1 over-expressed PC-3 cells; 3) To determine the tumorigenic capacity of ZIP1 over-expressed PC-3 cells; 4) To establish ZIP1 over-expressed LNCaP cells using stable transfection technique. At the present time the progress of this study is very promising, and we anticipate continuation of significant outcomes from this project. With this grant support, two abstracts were published and presented in international and local meetings, one paper was published and two manuscripts have been submitted.

Author: Manfred Wirth
Publisher: Walter de Gruyter
ISBN: 3110807270
Category : Medical
Languages : en
Pages : 220

View

Book Description

Author: Thomas Seyfried
Publisher: John Wiley & Sons
ISBN: 1118310306
Category : Science
Languages : en
Pages : 482

View

Book Description
The book addresses controversies related to the origins of cancer and provides solutions to cancer management and prevention. It expands upon Otto Warburg's well-known theory that all cancer is a disease of energy metabolism. However, Warburg did not link his theory to the "hallmarks of cancer" and thus his theory was discredited. This book aims to provide evidence, through case studies, that cancer is primarily a metabolic disease requring metabolic solutions for its management and prevention. Support for this position is derived from critical assessment of current cancer theories. Brain cancer case studies are presented as a proof of principle for metabolic solutions to disease management, but similarities are drawn to other types of cancer, including breast and colon, due to the same cellular mutations that they demonstrate.

Author: Scott M. Dehm
Publisher: Springer Nature
ISBN: 303032656X
Category : Medical
Languages : en
Pages : 483

View

Book Description
The purpose of this book is to provide a contemporary overview of the causes and consequences of prostate cancer from a cellular and genetic perspective. Written by experts in the fields of epidemiology, toxicology, cell biology, genetics, genomics, cell-cell interactions, cell signaling, hormone signaling, and transcriptional regulation, the text covers aspects of prostate cancer from disease initiation to metastasis. Chapters explore in depth the cells of origin for prostate cancer, its genomic subtypes, neural transcription factors in disease progression, epigenetic regulation of chromatin, and many other topics. This book distinguishes itself from other texts on prostate cancer by its focus on cellular and genetic mechanisms, as opposed to clinical diagnosis and management. As a result, this book will be of broad interest to basic and translational scientists with familiarity of these topics, as well as to trainees at earlier stages of their research careers.

Author: Anne Le
Publisher: Springer
ISBN: 331977736X
Category : Medical
Languages : en
Pages : 186

View

Book Description
Genetic alterations in cancer, in addition to being the fundamental drivers of tumorigenesis, can give rise to a variety of metabolic adaptations that allow cancer cells to survive and proliferate in diverse tumor microenvironments. This metabolic flexibility is different from normal cellular metabolic processes and leads to heterogeneity in cancer metabolism within the same cancer type or even within the same tumor. In this book, we delve into the complexity and diversity of cancer metabolism, and highlight how understanding the heterogeneity of cancer metabolism is fundamental to the development of effective metabolism-based therapeutic strategies. Deciphering how cancer cells utilize various nutrient resources will enable clinicians and researchers to pair specific chemotherapeutic agents with patients who are most likely to respond with positive outcomes, allowing for more cost-effective and personalized cancer therapeutic strategies.

Author: James P. Karr
Publisher: Springer Science & Business Media
ISBN:
Category : Medical
Languages : en
Pages : 408

View

Book Description
I. Intracellular Communications.- Tissue Specificity and Cell Death are Associated with Specific Alterations in Nuclear Matrix Proteins.- Mechanism of Growth Regulation in Androgen Responsive Cells.- The Impact of Androgen, Extracellular Matrix, and Stroma upon Proliferation and Differentiation of Benign and Malignant Prostate Epithelial Cells.- Therapeutic Approaches to Activating Programmed Cell Death of Androgen-Independent Prostatic Cancer Cells.- Cell Motility and Structural Harmonics in Prostate Cancer.- Panel Discussion.- II. Growth Factors - 1.- Studies of the Endocrine and Paracrine Effect of Tumor Produced Factors in Human Genitourinary Cancers.- Fibroblast Growth Factor: Implications in the Etiology of Benign Prostatic Hyperplasia.- Fibroblast-Mediated Human Epithelial Tumor Growth and Hormonal Responsiveness In Vivo.- Polyamine Requirement of Prostate Cancer Cell Proliferation.- Heparin-Binding (Fibroblast) Growth Factor/Receptor Gene Expression in the Prostate.- Characterization and Partial Purification of a Non - Heparin-Binding Prostate Growth Factor From Cancerous Human Prostate.- Panel Discussion.- Growth Factors - 2.- Transforming Growth Factor a: A Potential Autocrine Growth Regulator in Prostatic Carcinoma.- Prostatic Growth Factors (PrGFs) - From the Identification of Probasin to the Role of PrGFs.- Urogenital Sinus Derived Growth Inhibitory Factor.- Growth Factor Antagonists in Prostate Cancer: Suramin and Cytotoxic Polyamines as Potential Therapy.- Transforming Growth Factors in Human Prostate Cancer.- Gene Products as the Motivating Force in the Prostate Cell's Response to Androgens.- Panel Discussion.- III. Steroid Receptors.- Molecular Biology of Prostate - Specific Antigen.- Structure and Expression of the Androgen Receptor in Normal Tissues and in Prostate Carcinoma Cell Lines.- Structural Analysis and Gene Expression of TR2 Receptor and TR3 Receptor.- cDNA Cloning, Antibody Production and Immunohistochemical Localization of the Androgen Receptor.- New Approaches to Studies on the Androgen Receptor.- Specific Receptors for Vitamin D3 in Human Prostatic Carcinoma Cells.- Panel Discussion.- IV. Poster Presentations.- Role of Androgens and Extracellular Matrix in the Growth and Differentiation of Benign and Malignant Prostatic Epithelial Cells.- Tissue Specificity and Cell Death Are Associated with Specific Alterations in Nuclear Matrix Proteins.- ElTect of Transformation on Rat Prostatic Fibroblasts: Alterations In Extracellular Matrix and Cytoskeleton Gene Expression with Retention of Androgen Responsiveness and Androgen Receptor Expression.- A Potential Role for the MDR-1 Gene in the Development of Androgen-Independent Tumors.- Relevance of Low Androgen Levels and Adrenal Androgens in the Growth of Transplantable Human Prostatic Carcinomas.- Growth-Stimulating Effect of Growth Factor(s) from Androgen Independent Tumor Cells (CS 2-Cell) on Androgen Responsive Tumor Cells.- The Cellular Form of Human Prostatic Acid Phosphatase May Function as a Phosphotyrosyl Protein Phosphatase in Cells.- Expression of Prostate Antigen in LNCaP Cells in Culture.- Allelic Expression of the Mouse Ren-1 Genes in the Anterior Prostate (Coagulating Gland).- V. Dna Structure and Gene Expression.- Genomic Alterations in Prostatic Cancer.- Regulation of Gene Expression in the Prostate.- Androgen Regulation of HBGF I-(aFGF) and Characterization of the Androgen-Receptor mRNA in the Human Prostate Carcinoma Ceil Une - LNCaP/A-dep.- DNA Methylation, Differentiation and Cancer.- Evidence for tbe Involement of Genetic Differences and Mesenchymal Factors in the Progression of Oncogene - Induced Prostate Cancer in Reconstituted Mouse Prostate.- Differential Hybridization Analysis as a Tool to Study Prostatic Cancer Metastasis.- Molecular Biology of Androgen Acceptors in Prostatic Cancer Cells.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Panel Discussion.- Contributors.

Author: Donald J. Tindall
Publisher: Springer Science & Business Media
ISBN: 1461468280
Category : Medical
Languages : en
Pages : 575

View

Book Description
Prostate Cancer provides an up-to-date review of the biochemistry, molecular biology, and genetic changes in prostate cells that are the driving forces in the initiation and progression of cancer. It includes an overview by experts in the field of cell-cell interactions, including stem cells, reactive Stromal cells and membrane lipid rafts that are instrumental in the initiation and progression of prostate cancer.

Author: Nathalie Guylaine Bérubé
Publisher:
ISBN:
Category : Antioncogenes
Languages : en
Pages : 388

View

Book Description
To isolate the tumor suppressor gene encoded on chromosome 12, a smaller candidate region was identified by the use of radiation hybrid technology. Molecular methods subsequently led to the identification of chromosome 12 genes displaying increased expression in suppressed hybrids compared to tumorigenic hybrids. Novel genes and genes encoded by other chromosomes also demonstrated increased expression. We suggest that these genes are involved in the cellular pathway that leads to suppression in DU 145 prostate cancer cells upon the introduction of chromosome 12. Further analysis of the genes isolated in this study have the potential to serve as prognostic markers of prostate carcinogenesis.

Author: Thorsten Cramer
Publisher: Springer
ISBN: 3319421182
Category : Medical
Languages : en
Pages : 272

View

Book Description
This textbook presents concise chapters written by internationally respected experts on various important aspects of cancer-associated metabolism, offering a comprehensive overview of the central features of this exciting research field. The discovery that tumor cells display characteristic alterations of metabolic pathways has significantly changed our understanding of cancer: while the first description of tumor-specific changes in cellular energetics was published more than 90 years ago, the causal significance of this observation for the pathogenesis of cancer was only discovered in the post-genome era. The first 10 years of the twenty-first century were characterized by rapid advances in our grasp of the functional role of cancer-specific metabolism as well as the underlying molecular pathways. Various unanticipated interrelations between metabolic alterations and cancer-driving pathways were identified and currently await translation into diagnostic and therapeutic applications. Yet the speed, quantity, and complexity of these new discoveries make it difficult for researchers to keep up to date with the latest developments, an issue this book helps to remedy.

Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 62

View

Book Description
To develop an understanding of the molecular events that transform normal human prostate cells into prostate cancer, we have developed a system of cell transformation that permits the creation of immortalized and tumorigenic human prostate epithelial cell lines of defined genetic constitution. Expression of SV4O Large T antigen and hTERT, the catalytic subunit of telomerase, permitted immortalization. Transformation as assessed by the ability of these cells to form colonies in an anchorage independent fashion and to form tumors in immunodeficient host animals required the additional expression of an oncogenic version of the H-Ras protein. In addition, using hTERT alone, we have simultaneously created an immortalized human prostate stromal cell line. We have recently created human prostate epithelial cells expressing genes known to be altered in human prostate cancers (Myc, Akt) as well as the androgen receptor. These cell lines produce luminal prostate cancers when placed orthotopically in mice. These cell lines provide an important foundation for future studies that will allow us to investigate the precise molecular interactions that lead to the development of prostate cancer. Ultimately, the elucidation of these critical molecular determinants of prostate cancer will permit the identification and confirmation of important targets for future therapeutic intervention.