General Techniques of Cell Culture PDF Download

Author: Maureen A. Harrison
Publisher: Cambridge University Press
ISBN: 9780521574969
Category : Science
Languages : en
Pages : 178

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Book Description
Concise introduction to a major technique of cell biology laboratories for those new to the field.

Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 0521516358
Category : Medical
Languages : en
Pages : 769

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Book Description
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Author: Simon P. Langdon
Publisher: Springer Science & Business Media
ISBN: 1592594069
Category : Medical
Languages : en
Pages : 346

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Book Description
The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.

Author: Martin Clynes
Publisher: Springer Science & Business Media
ISBN: 3642804128
Category : Science
Languages : en
Pages : 624

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Book Description
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Author: Michael Aschner
Publisher: Humana
ISBN: 9781493992300
Category : Medical
Languages : en
Pages : 302

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Book Description
This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Category : Science
Languages : en
Pages : 247

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Book Description
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Author: Michael R. Davey
Publisher: John Wiley & Sons
ISBN: 9780470686515
Category : Science
Languages : en
Pages : 358

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Book Description
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

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Book Description

Author: Shohreh Amini
Publisher: Humana
ISBN: 9781071614396
Category : Medical
Languages : en
Pages : 205

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Book Description
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435

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Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.