Gel Permeation and Ion-Exchange Chromatography of Proteins and Peptides PDF Download

Author: Kato
Publisher: CRC Press
ISBN: 1466562846
Category : Science
Languages : en
Pages : 234

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Book Description
This book is a collection of critical reviews of the use of high-performance liquid chromatography in a very specialized area of research. It describes in detail modern methodology to separate nucleic acids, enzymes and a wide variety of biologically active proteins such as renin.

Author: Milton Hearn
Publisher: Elsevier
ISBN: 032315770X
Category : Science
Languages : en
Pages : 284

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Book Description
High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 1482231670
Category : Science
Languages : en
Pages : 417

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Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behaviour and how to determime appropriate operating conditions, this reference illustrates procedures, apparatus and types of ion exchanges, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, this text explains the effects of each variable on separation behaviour; compares models and equations describing behaviour of proteins in IEC with experimental results; introduces design calculation procedures for scale up, adapting IEC for individual requirements; discusses parameters affecting separation behaviour, including adsorption equilibria, stationary phase diffusion and axial dispersion; provides examples of applications, such as high or medium performance IEC and large scale operations and reviews both theorectical and experimental literature. This text serves as an reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists and biotechnologists.

Author: Colin T. Mant
Publisher: CRC Press
ISBN: 1351440756
Category : Medical
Languages : en
Pages : 960

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Book Description
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Author: Kato
Publisher: CRC Press
ISBN: 1000083179
Category : Science
Languages : en
Pages : 258

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Book Description
This book is a collection of critical reviews of the use of high-performance liquid chromatography in a very specialized area of research. It describes in detail modern methodology to separate nucleic acids, enzymes and a wide variety of biologically active proteins such as renin.

Author: Ram N. Gupta
Publisher: CRC Press
ISBN: 0429945507
Category : Science
Languages : en
Pages : 408

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Book Description
These volumes provide a reference source of different gas chromatographic, liquid chromatographic, or thin-layer chromatographic techniques for the qualitative determination of various therapeutic agents, including antibiotics, vitamins and hormones, drugs of abuse in body fluids, dosage forms, or food stuffs. Over 5000 publications were reviewed to prepare tables of chromatographic data for 800 compounds, arranged alphabetically by generic drug name or by drug groups. A detailed summary of the extraction procedure described in each publication included in the table of a particular drug is also provided. This easy-to-read handbook is useful for selecting an appropriate chromatographic procedure for the determination of a given compound according to the available facilities.

Author: Shawn Doonan
Publisher: Royal Society of Chemistry
ISBN: 9780854046928
Category : Education
Languages : en
Pages : 196

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Book Description
Encompassing all aspects of the structures of peptides and proteins, this book adopts a uniquely problem-oriented approach to the topic. Starting with a look at the structures and properties of the twenty amino acids that occur in proteins, and moving on to the synthesis of polypeptides and the isolation of proteins, Peptides and Proteins then addresses the methods of analysis of protein characteristics, including the modern methods of sequence analysis by mass spectrometry. Further chapters examine the three-dimensional nature of protein structure, and introduce the student to the use of computer applications (molecular graphics, databases, bioinformatics) in protein chemistry. Original research data is used in many of the problems, and throughout sufficient background biology is included, thus putting the subject into context for chemists. Aimed at first and second-year chemistry students, this title will also be of interest to students of biochemistry. Ideal for the needs of undergraduate chemistry students, Tutorial Chemistry Texts is a major new series consisting of short, single topic or modular texts concentrating on the fundamental areas of chemistry taught in undergraduate science courses. Each book provides a concise account of the basic principles underlying a given subject, embodying an independent-learning philosophy and including worked examples.

Author: Marie-Isabel Aguilar
Publisher: Springer Science & Business Media
ISBN: 1592597424
Category : Science
Languages : en
Pages : 411

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Book Description
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author: István Kerese
Publisher: Ellis Horwood
ISBN:
Category : Science
Languages : en
Pages : 384

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Book Description
Abstract: A reference text for food scientists, analysts, and biochemists presents a systematic, comprehensive survey of working procedures and methods for the analysis of proteins, peptides, and amino acids of varying origin and composition that can be used with equipment that generally is available in the average laboratory. Topics include: general laboratory methods for the preparation and characterization of proteinaceous materials; electrophoreticmethods (especially using polyacrylamide gels); thin-layer chromatographic methods; gel chromatography and gel filtration methods; and experimental procedures for nitrogendetermination, amino acid composition, electrophoresis, isoelectric focusing, ion-exchange chromatography, and gel filtration. Step-by-step descriptions are provided for sample preparation for analysis and problems requiring resolution are discussed.

Author: Helmut Determann
Publisher: Springer
ISBN: 3662287064
Category : Science
Languages : en
Pages : 205

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Book Description
The efforts spent on many a scientific book cannot be justified, no matter how many words are said about it. The opposite is true for this book and a few brief remarks upon its publication. Within a short period of time, short even by all present standards, gel chromatography has gone through a development and experienced an acceptance that is unknown to any other method. From experience, the new and unique separation technique is today known and liked in all laboratories that are concerned with substances of high molecular weight; in others, the technique is known from hearsay, the least. Soon it became evident that a comprehensive coverage of the conceptual development, the theoretical principles, and the experimental technique of the new method would be desirable. This coverage is now offered by the book of an expert. Its author has personally participated in the development from its beginning and helped to promote it. He has made possible the gel chromatography, also of proteins, on thin layer plates; for lipophilic substances he has contributed considerably to the transition from water to organic solvent systems and developed theoretical concepts for a better understanding of the effects that are responsible for the separation. The book, so it appears to me, is pointing in new directions. The reader does not only expect a clear presentation of facts but also that of instructions for practical applications. Both these expectations have been met by the expert.