Freeze Fracture PDF Download

Author: John E. Rash
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 226

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Author: Richard L. Roberts
Publisher: Oxford University Press, USA
ISBN: 9780195052282
Category : Electron microscopy
Languages : en
Pages : 430

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Book Description
The freeze fracture technique coupled with transmission electron microscopy has contributed significant scientific information toward the experimental investigation of both normal and abnormal cells in the fields of cellular, developmental and molecular biology, and to several subdisciplines in medicine, including pathology, anatomy, and physiology. This book presents a complete and up-to-date account of the macromolecular organization of membranes and the many membrane specialization of cells as well as overall cellular organization as reflected in tissues and organs. While the book emphasizes freeze fracture images and the useful scientific information contained in them, the authors have also included transmission electron micrographs of ultrathin sectioned cells, tissues, and organs in order to aid in the interpretation of the freeze fracture image and increase the book's utility. Where three dimensional views are particularly useful, scanning electron micrographs are included.

Author: Sek Wen Hui
Publisher: CRC Press
ISBN: 1351089056
Category : Medical
Languages : en
Pages : 470

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Book Description
This volume is a kind of celebration of the progress of freeze-fracture electron microscopy in recent years. Many of the authors are leaders of the advancing front. Instead of offering an instruction manual of how to perform new techniques or a review to recover what has happened in the past in respect fields, both which are abundantly available as journal articles and monographs, this volume is a collection of personal testimonies as examples of the power of the new freeze-fracture technology. Since each chapter also centres around specific biological problem, it also serves to illustrate how much the understanding of the problem has been advanced by the new freeze-fracture methodology, which is most cases is developed by the author(s) themselves. A characteristic of frontier development is that many chapters are dealing with controversial subjects. The Inclusion of these subjects in the volume represents the dynamic nature of the subject as viewed by the authors rather than the final verdicts of the subject matter.

Author: Nicholas J. Severs
Publisher: Wiley-Liss
ISBN:
Category : Medical
Languages : en
Pages : 392

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Book Description
The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.

Author: C. Stolinski
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 192

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Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

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Author: Rudolf A. Steinbrecht
Publisher: Springer Science & Business Media
ISBN: 3642728154
Category : Science
Languages : en
Pages : 308

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Book Description
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Author: T. Yasamura
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: J.K. Koehler
Publisher: Springer Science & Business Media
ISBN: 3642654924
Category : Science
Languages : en
Pages : 319

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Book Description
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Author: Masahiko Abe
Publisher: Springer
ISBN: 9811359318
Category : Science
Languages : en
Pages : 145

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Book Description
This book is a manual of measurement of colloids and interfaces designed especially for new researchers who have just begun research on these topics. The book is written by active researchers in the field of colloids and interfacial chemistry, based on the practical experience of the authors. In each chapter, the key points of measurement, how to analyze data correctly, points to be careful about, and merits of a particular method are concisely explained from the point of view of the readers. Not only in industries such as cosmetics and pharmaceuticals but also in academic studies of nanotechnology, correct understanding of colloid and interface phenomena is vital because the properties of these items, however small, are affected by the nature of interfaces. This book will be particularly useful for researchers who are not yet fully confident of the measurement techniques that are clearly explained here.