Flow Cytometry Basics for the Non-Expert PDF Download

Author: Christine Goetz
Publisher: Springer
ISBN: 3319980718
Category : Medical
Languages : en
Pages : 219

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Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.

Author: Howard M. Shapiro
Publisher: John Wiley & Sons
ISBN: 0471434035
Category : Science
Languages : en
Pages : 736

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Book Description
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Author: Alice Longobardi Givan
Publisher: John Wiley & Sons
ISBN: 1118688392
Category : Science
Languages : en
Pages : 309

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Book Description
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Author: Marion G. Macey
Publisher: Springer Science & Business Media
ISBN: 1597454516
Category : Medical
Languages : en
Pages : 289

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Book Description
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Author: Edmund S. Cibas
Publisher: Elsevier Health Sciences
ISBN: 1455750794
Category : Medical
Languages : en
Pages : 577

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Book Description
Take advantage of comprehensive yet concise coverage of nearly every type of cytology, including gynecologic, non-gynecologic, and FNA. Understand the clinical implications of your diagnoses and better equip yourself to make effective interpretations with clinical correlations emphasized throughout. Make the most informed reporting decisions through an in-depth analysis of common diagnostic pitfalls. Quickly find the answers you need with succinct, bulleted text, valuable clinical pearls, and useful summaries of key points. Gain real-life perspective on a complete range of cytologic findings with over 700 crystal-clear, full-color illustrations. Access expert knowledge and key insights into HPV testing; new billing and coding guidelines; patient management discussions; and much more, thanks to comprehensive updates throughout. See how to perform fine needle biopsy aspiration with a new 17-minute video available at Expert Consult. Narrated by pathologist Dr. Amy Ly, the video includes a patient interview, precautions, and demonstrations of techniques.

Author: Alexander E. Kalyuzhny
Publisher: Springer
ISBN: 3319308939
Category : Science
Languages : en
Pages : 91

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Book Description
Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.

Author: J Philip McCoy Jr
Publisher: Humana
ISBN: 9781493996490
Category : Medical
Languages : en
Pages : 375

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Book Description
This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Author: Michael J. Crawley
Publisher: John Wiley & Sons
ISBN: 9780470515068
Category : Mathematics
Languages : en
Pages : 953

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Book Description
The high-level language of R is recognized as one of the mostpowerful and flexible statistical software environments, and israpidly becoming the standard setting for quantitative analysis,statistics and graphics. R provides free access to unrivalledcoverage and cutting-edge applications, enabling the user to applynumerous statistical methods ranging from simple regression to timeseries or multivariate analysis. Building on the success of the author’s bestsellingStatistics: An Introduction using R, The R Book ispacked with worked examples, providing an all inclusive guide to R,ideal for novice and more accomplished users alike. The bookassumes no background in statistics or computing and introduces theadvantages of the R environment, detailing its applications in awide range of disciplines. Provides the first comprehensive reference manual for the Rlanguage, including practical guidance and full coverage of thegraphics facilities. Introduces all the statistical models covered by R, beginningwith simple classical tests such as chi-square and t-test. Proceeds to examine more advance methods, from regression andanalysis of variance, through to generalized linear models,generalized mixed models, time series, spatial statistics,multivariate statistics and much more. The R Book is aimed at undergraduates, postgraduates andprofessionals in science, engineering and medicine. It is alsoideal for students and professionals in statistics, economics,geography and the social sciences.

Author: Niclas Thomas
Publisher:
ISBN: 9781985665675
Category :
Languages : en
Pages : 158

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Book Description
Data science is a complex subject, but nevertheless one that can be made accessible to all through clear, intuitive explanations and worked examples. Existing software that forms the backbone of an immunologist's analytical toolkit (such as FlowJo and Prism) are expensive, inflexible and promotes a narrow mindset when it comes to analysing your data. On the other hand, the Python and R programming languages are open source, free and entirely customisable, giving the user the ability to implement any analysis they wish. Although programming languages can seem daunting to the uninitiated, it's far easier to learn than many immunologists may think. Rather than seeking to become an expert programmer, an understanding of the main concepts is more than enough to conduct your own bespoke analyses when coupled with a sound mathematical and statistical understanding. Our new book focusses on the practical aspects of data science, providing sufficient theoretical background without delving into all of the details of each of the methods presented. Introductory chapters are presented alongside the analysis of a publicly available data set, allowing the reader to have practical hands-on experience when learning about important concepts in statistics, machine learning and programming. Topics include: - How to build a predictive model How to visualise high-dimensional data Basics of programming in Python and R What techniques exist to cluster data Which statistics test to use/why/when What is dimension reduction; when and how to use it Once these fundamental topics have been covered, a number of case studies are presented, along with the underlying data, accompanying code and full explanations on topics such as automated, data-driven flow cytometry, building predictive models of disease using gene expression profiling and analysing high throughput sequencing data.

Author: Ivan Maurice Roitt
Publisher:
ISBN:
Category : Immunity
Languages : en
Pages : 244

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Book Description