Fast, Comprehensive Two-dimensional High Performance Liquid Chromatography PDF Download

Author: Dwight Robert Stoll
Publisher:
ISBN:
Category :
Languages : en
Pages : 546

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Author: Veronika R. Meyer
Publisher: John Wiley & Sons
ISBN: 1118681347
Category : Science
Languages : en
Pages : 342

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Book Description
Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “...provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - American Journal of Therapeutics

Author: Veronika R. Meyer
Publisher: John Wiley & Sons
ISBN: 0470682183
Category : Science
Languages : en
Pages : 434

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Book Description
Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

Author: Oliver Jones
Publisher: Springer Nature
ISBN: 9811561907
Category : Science
Languages : en
Pages : 86

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Book Description
This book addresses the growing interest in the field of two-dimensional liquid chromatography (2DLC), a powerful approach to increasing resolution, available peak capacity, and selectivity in analytical chromatography. 2DLC is suitable for many applications, including in the pharmaceutical and polymer industries and the omic sciences (metabolomics, lipidomics and proteomics). Thanks to recent advances in technology and software the instrumentation needed to perform 2D-LC is broadly available to the analytical community in both industry and academia. Indeed, the technique can now be considered ready for application in R&D as well as in QA and QC labs, yet it is not widely known about outside academic laboratories and is rarely taught at the undergraduate level. This book outlines the main principles and features of 2D-LC (including comprehensive and heart-cutting modes, method development and real world applications) to enable modern analysts to start using this fascinating technique. The book offers an ideal starting point for those wishing to get into 2D-LC and will also be of interest to more experienced scientists in the field.

Author: R. Hamilton
Publisher: Springer Science & Business Media
ISBN: 9400959389
Category : Science
Languages : en
Pages : 256

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Book Description
Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation. The use of microprocessor control has brought about a rationalization of mobile phase delivery systems and in detectors, the introduction of electrochemical and spectrophotometric detection other than in the ultra-violet region, has widened the field of applications and the sensitivity of the technique. The use of ion pair chromatography has increased at the expense of ion-exchange and this together with the improvements in detectors has greatly increased the appli cation of the technique in the biomedical field. These advances are described together with the established methods to enable the beginner to carry out a satisfactory separation and to gain the experience necessary for the full exploitation of the technique. R. J. Hamilton P. A. Sewell Liverpool,1981 1 Introduction to high performance liquid chromatography 1. 1 Introduction Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Uquid chromatography in the of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel form filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates.

Author: Dwight R. Stoll
Publisher: CRC Press
ISBN: 1000817334
Category : Science
Languages : en
Pages : 401

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Book Description
Two-dimensional liquid chromatography (2D-LC) is finding increasingly wide application principally due to the analysis of mixtures of moderate to high complexity. Many industries are developing increasingly complex products that are challenging the separation capabilities of state-of-the-art 1D-LC and need new analytical methodologies with substantially more resolving power, and 2D-LC meets that need. This text, organized by two leaders in the field, establishes a sound fundamental basis for the principles of the technique, followed by a discussion of important practical considerations. The book begins with an introduction to multi-dimensional separations and a discussion of the history and development of the technique over the past 40 years, followed by several chapters that provide a theoretical basis for development of 2D-LC methods, including foundational concepts regarding separation complementarity, under-sampling, and dynamics of liquid chromatography separations. Instrumentation for 2D-LC is discussed extensively, including practical aspects such as interface selection and setup. Building on this foundation, two separate chapters are focused on method development for non-comprehensive and comprehensive separations, followed by a chapter dedicated to data analysis. Finally, applications of 2D-LC in several fields ranging from pharmaceutical analysis to polymer science are summarized. The book is an important resource for both students and practitioners who are already using 2D-LC or are interested in getting started in the field. Key Features: Demonstrates the conditions under which a 2D-LC method should be considered as an alternative to a 1D-LC method Establishes a sound fundamental basis of the principles of the technique, followed by guidelines for method optimization Provides a single source for technical knowledge advances and practical guidance described in recent literature Assists with the initial decision to develop a 2D-LC method Guides the reader in developing a high-quality method that meets the needs of their application

Author: Steven A. Cohen
Publisher: John Wiley & Sons
ISBN: 0470276258
Category : Science
Languages : en
Pages : 490

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Book Description
Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.

Author: Michal Holcapek
Publisher: Elsevier
ISBN: 0128117338
Category : Technology & Engineering
Languages : en
Pages : 522

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Book Description
Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. - Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more - Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques - Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research

Author: Sandie Lindsay
Publisher: John Wiley & Sons
ISBN: 0471931152
Category : Science
Languages : en
Pages : 370

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Book Description
High performance liquid chromatography is the most powerful of all the chromatographic techniques, often achieving separations and analyses that would be difficult or impossible with other forms of chromatography. This study and training text examines the concepts and techniques used in this field. A selection of literature available from equipment manufacturers is included along with a brief review of some more specialized topics.

Author: Luigi Mondello
Publisher: John Wiley & Sons
ISBN: 9780471988694
Category : Science
Languages : en
Pages : 464

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Book Description
Mehrdimensionale Chromatographie im analytischen Labor: Dieses Buch bespricht erstmals alle gängigen Verfahren sowie Anwendungen auf verschiedensten Gebieten, von der Pharmazie, Biologie und Chemie bis hin zur Umwelttechnik und erdölverarbeitender Industrie. Die Autoren sind selbst aktiv in der einschlägigen Forschung tätig.